Physiological Responses to Blood Collection Methods in Rats

Besch, E. L.; Chou, Billy J.

doi:10.3181/00379727-138-36041

Cited by 36 publications

(20 citation statements)

References 2 publications

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“…The volume of blood sampled also has an impact on the heart function: we observed a 12.5% decrease in the left ventricle ejection fraction between the single blood sampling (59.7±4.4%) and the dual blood sampling (unpaired t-test, P = 0.0013). Circulatory shock had an impact on the cardiac function and this is fairly well correlated to what we observe in our simultaneous experiment [7,9,30].…”

Section: Simultaneous Versus Single Artery Withdrawalsupporting

confidence: 88%

“…It is also challenging to obtain the AIF in small animals because of the limited amount of blood that can be withdrawn. For example, in rats the total blood volume is estimated to be 6.4 ml/100 g [6] and it is not recommended to remove more than 10% of that volume so as not to alter the basal metabolism and avoid circulatory shock [7][8][9]; this is a significant limiting factor for the manual withdrawal method.…”

Section: Introductionmentioning

confidence: 99%

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Arterial input function sampling without surgery in rats for positron emission tomography molecular imaging

Croteau

Poulin

Tremblay

et al. 2014

Nuclear Medicine Communications

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Section: Simultaneous Versus Single Artery Withdrawalsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Arterial input function sampling without surgery in rats for positron emission tomography molecular imaging

Croteau

Poulin

Tremblay

et al. 2014

Nuclear Medicine Communications

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“…Thus, it is required that when shifting to a new method of anaesthesia/euthanasia in similar studies, data migration should be mitigated for the least impact possible to maintain continuity throughout these studies. However, ether itself is known to affect the physiological parameters of laboratory animals to an unignorable extent [3,13]. As such, departure from ether is embracing the contradiction that if animal stress is tightly related to the fluctuation of parameters, it might be difficult to remove only the stress while keeping continuous data.…”

mentioning

confidence: 99%

Diluted Isoflurane as a Suitable Alternative for Diethyl ether for Rat Anaesthesia in Regular Toxicology Studies

Nagate

Chino

Nishiyama

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Despite its explosive properties and toxicity to both animals and humans, diethyl ether is an agent long used in Japan in the anaesthesia jar method of rat anaesthetises. However, in response to a recent report from the Science Council of Japan condemning diethyl ether as acceptable practice, we searched for an alternative rat anaesthesia method that provided data continuous with pre-existing regular toxicology studies already conducted under diethyl ether anaesthesia. For this, we examined two candidates; 30% isoflurane diluted with propylene glycol and pentobarbitone. Whereas isoflurane is considered to be one of the representatives of modern volatile anaesthetics, the method of propylene glycol-diluted 30% isoflurane used in this study was our modification of a recently reported method revealed to have several advantages as an inhalation anaesthesia. Intraperitoneal pentobarbitone has long been accepted as a humane method in laboratory animal anaesthesiology. These 2 modalities were scrutinized in terms of consistency of haematology and blood chemistry with previous results using ether. We found that pentobarbitone required a much longer induction time than diethyl ether, which is suspected to be the cause of fluctuations in several haematological and blood chemical results. Conversely, only calcium ion concentration showed a slight difference from traditional results in the case of 30% isoflurane. Additionally, serum prolactin and corticosterone levels indicated that 30% isoflurane induced less stress than ether, confirming that 30% isoflurane can both provide results consistent with diethyl ether, while at the same time remove its disadvantages. As such 30% isoflurane appears to be a strong alternative anaesthetic agent for future regular toxicology studies in Japan.

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“…probably elevated by handling and by the brief anaesthesia [8]. The plasma insulin concentrations fell during the day and were still low after 24 hrs.…”

Section: Methodsmentioning

confidence: 95%

Effects of metformin on insulin resistance after injury in the rat

Frayn

1976

Diabetologia

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Summary. Hyperglycaemia and insulin resistance occur after injury. The effects of the antidiabetic biguanide metformin in injured rats have been studied in order to elucidate the cause of these effects. Metformin (120 mg/kg S. C.) produced a significant hypoglycaemic effect after a 20 % dorsal scald but did not affect the blood glucose concentration in non-injured rats. The hypoglycaemic effect did not result from increased insulin secretion. It was associated with a reduction in liver glycogen and an increase in blood lactate concentrations, suggesting that the drug acted by promoting peripheral glucose utilization. This was confirmed by measuring the clearance rate coefficient of [5-3H]glucose. This rate coefficient was significantly increased by metformin treatment (140 mg/kg S. C.) in scalded rats, although it was not affected in non-injured rats. Intravenous glucose tolerance in scalded rats was not improved, probably because of the increased lactate concentration. Metformin (120-160 mg/kg) also produced a hypoglycaemic effect in rats after a 4 hrs period of bilateral hind-limb ischaemia, suggesting that similar metabolic changes occur after these two types of injury.

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Physiological Responses to Blood Collection Methods in Rats

Cited by 36 publications

References 2 publications

Arterial input function sampling without surgery in rats for positron emission tomography molecular imaging

Arterial input function sampling without surgery in rats for positron emission tomography molecular imaging

Diluted Isoflurane as a Suitable Alternative for Diethyl ether for Rat Anaesthesia in Regular Toxicology Studies

Effects of metformin on insulin resistance after injury in the rat

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