Physiological Role of Ascorbic Acid Recycling Enzymes in Plants

Suekawa, Marina; Fujikawa, Yukichi; Esaka, Muneharu

doi:10.1007/978-3-319-74057-7_14

Cited by 8 publications

(8 citation statements)

References 78 publications

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“…Moreover, MDHA is unstable unless it produces AsA and DHA. MDHA and DHA are directly reduced to AsA by two reductases: monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) (Park et al 2016; Suekawa et al 2017). If DHA is not rapidly reduced enzymatically to AsA, it may be lost from the cell (Smirnoff2018).…”

Section: Results and Discusionmentioning

confidence: 99%

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Wang

et al. 2020

Plant Biol J

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Few relevant research attempts have been made to determine heavy metal resistance mechanisms of rhizomatous perennial plants. Thus, it is pertinent to investigate the physiological and biochemical changes in Phragmites australis under metal‐stressed conditions to facilitate the development of strategies to enhance copper (Cu) tolerance. We measured parameters related to plant growth and development, metal translocation and physiological responses of P. australis subjected to Cu stress. In addition, the differentially expressed proteins (DEP) were evaluated using the isobaric tag for relative and absolute quantification (iTRAQ) system. A large amount of copper accumulates in the roots of P.australis, but the growth parameters were not sensitive to Cu. However, the high concentration of Cu reduced the content of chlorophyll a and chlorophyll b, and the expression of important photosynthesis proteins PsbD, PsbO and PsaA were all down‐regulated, so photosynthesis was inhibited. In contrast, the content of ascorbic acid and proline both increased with the increase of copper stress. P.australis fixed a large amount of Cu in its roots, limiting the migration of Cu to other parts of the plant. Moreover, Cu stress can affect photosynthesis by inhibiting the activity of PSI, PSII and LHCII. In addition, P.australis synthesizes ascorbic acid through the D‐mannose/L‐galactose pathway, and synthesizes proline through the ornithine pathway. Ascorbic acid and proline can increase Cu tolerance and protect photosynthesis. These results provide a theoretical basis for understanding the tolerance and repair mechanisms of plants in response to heavy metal pollution.

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Section: Results and Discusionmentioning

confidence: 99%

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Wang

et al. 2020

Plant Biol J

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“…MDHA and DHA again get reduced to AsA by monodehydroascorbate reductase and DHA reductase, respectively. This AsA-DHA cycle is highly important for plant growth and development (Suekawa et al, 2018), as well as (a)biotic stress tolerance (Boubakri, 2018;Veljović-Jovanović et al, 2018).…”

Section: Examples Of Ir Stimulants Include Beneficial Microbes Such Asmentioning

confidence: 99%

“…MDHA and DHA again get reduced to AsA by monodehydroascorbate reductase and DHA reductase, respectively. This AsA–DHA cycle is highly important for plant growth and development (Suekawa et al, 2018 ), as well as (a)biotic stress tolerance (Boubakri, 2018 ; Veljović‐Jovanović et al, 2018 ). DHA performs unique functions like cell cycle progression sensing and regulation, and modulation of metal stress responses, and DHA adducts seem to be involved in oxidative stress‐mediated cellular toxicity (Miret & Müller, 2018 ; Potters et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Chavan

Kesel

Desmedt

et al. 2022

Molecular Plant Pathology

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Ascorbic acid (AsA) is an important antioxidant in plants and regulates various physiological processes. In this study, we show that exogenous treatments with the oxidized form of AsA, that is, dehydroascorbate (DHA), activates induced systemic resistance in rice against the root‐knot nematode Meloidogyne graminicola , and investigate the molecular and biochemical mechanisms underlying this phenotype. Detailed transcriptome analysis on roots of rice plants showed an early and robust transcriptional response on foliar DHA treatment, with induction of several genes related to plant stress responses, immunity, antioxidant activity, and secondary metabolism already at 1 day after treatment. Quantitative and qualitative evaluation of H 2 O 2 levels confirmed the appearance of a reactive oxygen species (ROS) burst on DHA treatment, both at the site of treatment and systemically. Experiments using chemical ROS inhibitors or scavengers confirmed that H 2 O 2 accumulation contributes to DHA‐based induced resistance. Furthermore, hormone measurements in DHA‐treated plants showed a significant systemic accumulation of the defence hormone salicylic acid (SA). The role of the SA pathway in DHA‐based induced resistance was confirmed by nematode infection experiments using an SA‐signalling deficient WRKY45 ‐RNAi line and reverse transcription‐quantitative PCR on SA marker genes. Our results collectively reveal that DHA activates induced systemic resistance in rice against the root‐knot nematode M. graminicola , mediated through the production of ROS and activation of the SA pathway.

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“…Indeed, VC has been shown to ameliorate the negative effects of high salinity and heavy metal exposure in agricultural crops [ 67 , 69 , 70 , 71 , 72 ]. The protective aspects of VC are mostly relevant to the apoplast, a plant matrix considered to be the primary target of external abiotic or biotic factors in the roots [ 73 , 74 ], the chloroplast [ 75 , 76 ], and the organelle, where important physiological processes such as photosynthesis and lipid metabolism occur [ 77 ]. In simplistic terms, in response to stress factors that induce oxidative stress, such as exposure to ozone, VC can be released from the cytoplasm of plant cells to the apoplast, through the plasma membrane, where it becomes enzymatically or non-enzymatically oxidized, while scavenging radical species [ 73 ].…”

Section: Introductionmentioning

confidence: 99%

“…In simplistic terms, in response to stress factors that induce oxidative stress, such as exposure to ozone, VC can be released from the cytoplasm of plant cells to the apoplast, through the plasma membrane, where it becomes enzymatically or non-enzymatically oxidized, while scavenging radical species [ 73 ]. In the apoplast, where the conditions are considerably acidic (pH between 5 and 6) and dehydroascorbate reductase is absent, the originated MDHA is very unstable and the capacity to regenerate VC from DHA is impaired, and thus the main metabolite that is present is DHA [ 73 , 75 ]. Although the research is limited, this high concentration of DHA can potentially interfere in relevant physiological mechanisms, such as the regulation of protein activity in the plasma membrane and cell wall [ 73 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in Novel Animal Vitamin C Biosynthesis Pathways and the Role of Prokaryote-Based Inferences to Understand Their Origin

Duque

Vieira

2022

Genes

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Vitamin C (VC) is an essential nutrient required for the optimal function and development of many organisms. VC has been studied for many decades, and still today, the characterization of its functions is a dynamic scientific field, mainly because of its commercial and therapeutic applications. In this review, we discuss, in a comparative way, the increasing evidence for alternative VC synthesis pathways in insects and nematodes, and the potential of myo-inositol as a possible substrate for this metabolic process in metazoans. Methodological approaches that may be useful for the future characterization of the VC synthesis pathways of Caenorhabditis elegans and Drosophila melanogaster are here discussed. We also summarize the current distribution of the eukaryote aldonolactone oxidoreductases gene lineages, while highlighting the added value of studies on prokaryote species that are likely able to synthesize VC for both the characterization of novel VC synthesis pathways and inferences on the complex evolutionary history of such pathways. Such work may help improve the industrial production of VC.

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Physiological Role of Ascorbic Acid Recycling Enzymes in Plants

Cited by 8 publications

References 78 publications

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Responses of Phragmites australis to copper stress: A combined analysis of plant morphology, physiology and proteomics

Dehydroascorbate induces plant resistance in rice against root‐knot nematode Meloidogyne graminicola

Advances in Novel Animal Vitamin C Biosynthesis Pathways and the Role of Prokaryote-Based Inferences to Understand Their Origin

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