Physiological role of endogenous S-adenosyl-L-methionine synthetase in Chinese cabbage

Yu, Jae-Gyeong; Lee, Gi-Ho; Park, Young-Doo

doi:10.1007/s13580-012-0021-7

Cited by 16 publications

(6 citation statements)

References 30 publications

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“…Methionine adenosyltransferase (MAT) was detected as in vitro S -nitrosated during PEMs proliferation only in the B2 cell line ( Table 2 ). MAT and Adenosylhomocysteinase are part of the methylMet cycle which provides activated methyl groups in transmethylation reactions ( Yu et al, 2012 ). MAT (also known as S-adenosylmethionine synthetase, SAMS), catalyzes the biosynthesis of S-adenosylmethionine (AdoMet), a precursor for ethylene and polyamines biosynthesis in plants.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze

et al. 2022

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Cysteine S-nitrosation is a redox-based post-translational modification that mediates nitric oxide (NO) regulation of various aspects of plant growth, development and stress responses. Despite its importance, studies exploring protein signaling pathways that are regulated by S-nitrosation during somatic embryogenesis have not been performed. In the present study, endogenous cysteine S-nitrosation site and S-nitrosated proteins were identified by iodo-TMT labeling during somatic embryogenesis in Brazilian pine, an endangered native conifer of South America. In addition, endogenous –S-nitrosothiol (SNO) levels and S-nitrosoglutathione reductase (GSNOR) activity were determined in cell lines with contrasting embryogenic potential. Overall, we identified an array of proteins associated with a large variety of biological processes and molecular functions with some of them already described as important for somatic embryogenesis (Class IV chitinase, pyruvate dehydrogenase E1 and dehydroascorbate reductase). In total, our S-nitrosoproteome analyses identified 18 endogenously S-nitrosated proteins and 50 in vitro S-nitrosated proteins (after GSNO treatment) during cell culture proliferation and embryo development. Furthermore, SNO levels and GSNOR activity were increased during embryo formation. These findings expand our understanding of the Brazilian pine proteome and shed novel insights into the potential use of pharmacological manipulation of NO levels by using NO inhibitors and donors during somatic embryogenesis.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze

et al. 2022

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“…The data showing a role of gaseous plant hormoneethylene in in vitro callus growth, organo-and embryogenesis has been reported quite frequently (Kumar et al 2009). Additionally, it was proven that changes in the SAMS expression level strongly affected genes associated with defense response to abiotic stress, ethylene and jasmonic acid synthesis (Yu et al 2012). Therefore higher levels of SAMS in anthers isolated from responsive DH lines after LT treatment are possibly not only the reaction to LT stress but also impose direct effect on ME induction.…”

Section: Discussionmentioning

confidence: 99%

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

et al. 2017

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For a better understanding of the physiological background of microspore embryogenesis (ME), the protein profile was analyzed in four winter triticale DH lines, which show extremely different embryogenic potential. The analysis were conducted with anthers at the phase of development optimal for ME induction and then after low temperature (LT, 3 weeks at 4°C) MEinducing tillers treatment. The sub-proteome of anthers was mapped by two-dimensional gel electrophoresis (2-DE). The protein species significantly more abundant (at least 2-fold) in responsive DH lines after LT treatment were chosen for identification by MALDI-TOF/TOF analysis. In total, 31 protein species were successfully identified as involved in the determination of microspore competence, stress response and in the regulation of ME induction. Microspore competence required sufficient energy supply and efficient system of cell protection that determine survival under prolonged LT stress treatment. LT stress was associated with increased accumulation of proteins typical for cell defence against oxidative stress (e.g., L-ascorbate peroxidase), chaperons (e.g., HSP70) and other enzymes/factors ensuring protein biosynthesis, stability and active cell divisions. Also here, effective cell defence required undisturbed energy supply. Among proteins that accumulated differentially in accordance with microspore embryogenic potential again the most important role seems to be played by the enzymes ensuring energy production and determining ability of plant stress adaptation. Two protein species (enolase, 12S storage protein), proposed earlier as candidates for markers of embryogenesis in other in vitro plant culture systems confirmed their utility for triticale anther cultures.

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“…SAM (S-adenosylmethionine synthetase) catalyzes the nal step in the synthesis of the molecule. Altered expression of SAMs not only affects expression of genes related to abiotic stress [64][65][66][67], but also modi es the methylation status of DNA. In detail, missense mutation of SAM3/MAT4 in Arabidopsis was shown to result in decreased CHG and CHH DNA methylation [68].…”

Section: Beta Vulgaris Alters Global Methylation Level In Response To...mentioning

confidence: 99%

Multi-Omics Data Integration Reveals Link Between Epigenetic Modifications and Gene Expression in Sugar Beet (Beta Vulgaris Subsp. Vulgaris) in Response to Cold

Gutschker

Corral

Schmiedl

et al. 2021

Preprint

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BackgroundDNA methylation is thought to influence the expression of genes, especially in response to changing environmental conditions and developmental changes. Sugar beet (Beta vulgaris ssp. vulgaris), and other biennial or perennial plants are inevitably exposed to fluctuating temperatures throughout their lifecycle and might even require such stimulus to acquire floral competence. Therefore, plants such as beets, need to fine-tune their epigenetic makeup to ensure phenotypic plasticity towards changing environmental conditions while at the same time steering essential developmental processes. Different crop species may show opposing reactions towards the same abiotic stress, or, vice versa, identical species may respond differently depending on the specific kind of stress. ResultsIn this study, we investigated common effects of cold treatment on genome-wide DNA methylation and gene expression of two Beta vulgaris accessions via multi-omics data analysis. Cold exposure resulted in a pronounced reduction of DNA methylation levels, which particularly affected methylation in CHH context (and to a lesser extent CHG) and was accompanied by transcriptional downregulation of the chromomethyltransferase CMT2 and strong upregulation of several genes mediating active DNA demethylation. Conclusion Integration of methylomic and transcriptomic data revealed that, rather than methylation having directly influenced expression, epigenetic modifications correlated with changes in expression of known players involved in DNA (de)methylation. In particular, cold triggered upregulation of genes putatively contributing to DNA demethylation via the ROS1 pathway. Our observations suggest that these transcriptional responses precede the cold-induced global DNA-hypomethylation in non-CpG, preparing beets for additional transcriptional alterations necessary for adapting to upcoming environmental changes.

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Physiological role of endogenous S-adenosyl-L-methionine synthetase in Chinese cabbage

Cited by 16 publications

References 30 publications

Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze

Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

Multi-Omics Data Integration Reveals Link Between Epigenetic Modifications and Gene Expression in Sugar Beet (Beta Vulgaris Subsp. Vulgaris) in Response to Cold

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