Physiological Role of Placental Proteases: Interaction Between Pregnancy-Induced Bioactive Peptides and Proteases

Mizutani, Shigehiko; Goto, Keiko; Itakura, Atsuo; Furuhashi, Madoka; Kurauchi, O.; Kikkawa, Fumitaka; Tsutsumi, Yutaka

doi:10.1507/endocrj.41.supplement_s93

Cited by 14 publications

(5 citation statements)

References 56 publications

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“…The fetus is known to produce vasopressin (De Vane and Porter 1980) and angiotensin (Broughton Pipkin and Symonds 1977) and it has been reported that the increased vasopressinase (P-LAP) and angiotensinase (Aminopeptidase A, AP-A) activities of pregnancy serum may originate from placenta (Mizutani et al 1994a). It is also known that vasopressin and angiotensin are potent regulators of fetal blood pressure in normal and aberrant conditions (Reid 1992).…”

Section: Discussionmentioning

confidence: 99%

Relationship Between the Changes in Maternal Serum Placental Leucine Aminopeptidase (P-LAP) Activity and Umbilical Artery Waveforms in Normal Pregnancy

Mohamed¹,

Mizutani²,

Itakura³

et al. 1996

Horm Metab Res

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Section: Discussionmentioning

confidence: 99%

Relationship Between the Changes in Maternal Serum Placental Leucine Aminopeptidase (P-LAP) Activity and Umbilical Artery Waveforms in Normal Pregnancy

Mohamed¹,

Mizutani²,

Itakura³

et al. 1996

Horm Metab Res

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“…APN/CD13 is a type II 150-kDa membrane metalloprotease with an extracellularoriented catalytic domain. APN cleaves the N-terminal neutral residue of physiological peptides, and ubiquitously functions in various peptide metabolism pathways [45]. While DPPIV was detected in EECs, APN/CD13 was detected in ESCs and decidual cells [5].…”

Section: Apn/cd13 In Implantationmentioning

confidence: 99%

Recent Insights into Human Endometrial Peptidases in Blastocyst Implantation via Shedding of Microvesicles

Yoshihara

Mizutani²,

Kato

et al. 2021

IJMS

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Blastocyst implantation involves multiple interactions with numerous molecules expressed in endometrial epithelial cells (EECs) during the implantation window; however, there is limited information regarding the molecular mechanism underlying the crosstalk. In blastocysts, fibronectin plays a major role in the adhesion of various types of cells by binding to extracellular matrix proteins via the Arg-Gly-Asp (RGD) motif. In EECs, RGD-recognizing integrins are important bridging receptors for fibronectin, whereas the non-RGD binding of fibronectin includes interactions with dipeptidyl peptidase IV (DPPIV)/cluster of differentiation (CD) 26. Fibronectin may also bind to aminopeptidase N (APN)/CD13, and in the endometrium, these peptidases are present in plasma membranes and lysosomal membranes. Blastocyst implantation is accompanied by lysosome exocytosis, which transports various peptidases and nutrients into the endometrial cavity to facilitate blastocyst implantation. Both DPPIV and APN are released into the uterine cavity via shedding of microvesicles (MVs) from EECs. Recently, extracellular vesicles derived from endometrial cells have been proposed to act on trophectoderm cells to promote implantation. MVs are also secreted from embryonal stem cells and may play an active role in implantation. Thus, crosstalk between the blastocyst and endometrium via extracellular vesicles is a new insight into the fundamental molecular basis of blastocyst implantation.

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“…It is known that by constricting the umbilical artery and vein and the ductus arteriosus while dilating the pulmonary circulation, bradykinin (BK) is capable of mediating some of the rapid circulatory changes required of a neonate possibly under acute hypoxia [1]. As with other locally acting tissue hormones such as angiotensin II (ANG II) and oxytocin, the activity of BK is determined by factors leading to their degradation [2]. We reported that kininase I, kininase II (angiotensin converting enzyme, ACE) and endopeptidase 24.11 (CD10) appeared to be the most important in the degradation of BK in the placenta ± while ACE is located primarily in umbilical venous endothelial cells, CD10 is located in villous trophoblast [3 ± 6].…”

Section: Introductionmentioning

confidence: 99%

Umbilical Plasma Kininase I Activity in Fetal Hypoxia

Tsukahara

Itakura²,

Ohno³

et al. 2003

Horm Metab Res

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To shed light on the role of bradykinin in preeclampsia in addition to acute hypoxia, we measured the activity of kininase I, the enzyme responsible for its degradation, in umbilical plasma. Kininase I activity in umbilical arteries was compared with that in the umbilical veins. The relationship between kininase I and pH values was also evaluated in women with and without preeclampsia. Also, enzyme activity in supernatants of fetal hepatic cells (NFL/T) cultured under hypoxic or normoxic conditions were determined. Kininase I activity levels in fetal umbilical arteries and veins (n = 33) were similar (r = 0.77). Hypoxia caused suppression of kininase I activity in the supernatant cultures of NFL/T after one hour. However, after 8 and 24 hours, kininase I activity was significantly greater than under normoxic conditions (p < 0.05). Kininase I activity of fetal umbilical vein significantly decreased in the presence of acidemia in the uncomplicated group (n = 75, r = 0.42), whereas the activity negatively correlated with umbilical arterial pH in the preeclamptic group (n = 10, r = - 0.65). Kininase I activity levels in cases complicated with preeclampsia were significantly higher than without preeclampsia (49.2 +/- 9.1 vs. 66.2 +/- 11.3 nmol/ml/min). The present study indicates that kininase I acts as a regulatory factor in bradykinin degradation.

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Physiological Role of Placental Proteases: Interaction Between Pregnancy-Induced Bioactive Peptides and Proteases

Abstract: Abstract.We found 9 proteases in human placenta. Our studies showed that these placental proteases metabolize vasoactive and immunomodulating peptides, possibly derived from the fetus, and protect

Cited by 14 publications

References 56 publications

Relationship Between the Changes in Maternal Serum Placental Leucine Aminopeptidase (P-LAP) Activity and Umbilical Artery Waveforms in Normal Pregnancy

Relationship Between the Changes in Maternal Serum Placental Leucine Aminopeptidase (P-LAP) Activity and Umbilical Artery Waveforms in Normal Pregnancy

Recent Insights into Human Endometrial Peptidases in Blastocyst Implantation via Shedding of Microvesicles

Umbilical Plasma Kininase I Activity in Fetal Hypoxia

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