Physiological, transcriptional, and metabolic alterations in spaceflight-subjected Senna obtusifolia

Mao, Renjun; Li, Wenrui; He, Zhigui; Bai, Zhenqing; Xia, Pengguo; Liang, Zongsuo; Liu, Yan

doi:10.1016/j.plaphy.2019.03.009

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…TFs interact with target gene promoters to activate or inhibit target gene function. Correlation analysis can be used to confirm relationships between the regulation of gene expression and related biochemical properties in plants [29,30]. According to GO annotation, 15 genes were divided into two categories, namely, enzymes and transcription factors.…”

Section: Discussionmentioning

confidence: 99%

Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

Fan

et al. 2021

Biology

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The study of somatic embryogenesis can provide insight into early plant development. We previously obtained LaMIR166a-overexpressing embryonic cell lines of Larix kaempferi (Lamb.) Carr. To further elucidate the molecular mechanisms associated with miR166 in this species, the transcriptional profiles of wild-type (WT) and three LaMIR166a-overexpressing transgenic cell lines were subjected to RNA sequencing using the Illumina NovaSeq 6000 system. In total, 203,256 unigenes were generated using Trinity de novo assembly, and 2467 differentially expressed genes were obtained by comparing transgenic and WT lines. In addition, we analyzed the cleaved degree of LaMIR166a target genes LaHDZ31–34 in different transgenic cell lines by detecting the expression pattern of LaHdZ31–34, and their cleaved degree in transgenic cell lines was higher than that in WT. The downstream genes of LaHDZ31–34 were identified using Pearson correlation coefficients. Yeast one-hybrid and dual-luciferase report assays revealed that the transcription factors LaHDZ31–34 could bind to the promoters of LaPAP, LaPP1, LaZFP5, and LaPHO1. This is the first report of gene expression changes caused by LaMIR166a overexpression in Japanese larch. These findings lay a foundation for future studies on the regulatory mechanism of miR166.

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Section: Discussionmentioning

confidence: 99%

Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

Fan

et al. 2021

Biology

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“…In an earlier study, based on experimental results and literature reports, we speculated that peroxidase ( POD ) and ascorbate peroxidase ( APX ) are involved in regulating AO accumulation and lead to a higher AO content in QC29 [ 20 ]. Here, transcriptome results indicated that 12 unigenes were identified as antioxidant enzyme genes, among which three POD (Cluster-26.44407, Cluster-26.33814, Cluster-26.46919) and two APX (Cluster-26.50084, Cluster-26.49222) genes were identified as DEGs in QC29.…”

Section: Discussionmentioning

confidence: 99%

“…The relative expression levels were calculated using the 2 −ΔΔCt method. The detailed method was described in our previous study [ 20 ]. Briefly, qRT–PCR analysis was performed on a CFX96 Real-Time System (BioRad, USA) using SYBR Premix Ex TaqII (TaKaRa, Dalian, China) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Although our previous studies have demonstrated that SP-lines have higher antioxidant enzyme activity and corresponding gene transcripts [ 20 ] and AO content [ 20 , 21 ], the underlying mechanism is still unknown. In this study, we used transcriptome sequencing combined with HPLC to (1) compare the differences between SP-lines and GC3 at the transcriptional and metabolic levels, (2) reveal the underlying mechanisms of the higher AO content in SP-lines, and (3) provide a specific transcriptome database of S. obtusifolia seeds.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome and HPLC Analysis Reveal the Regulatory Mechanisms of Aurantio-Obtusin in Space Environment-Induced Senna obtusifolia Lines

Mao

Bai

et al. 2022

IJERPH

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Senna obtusifolia is a famous medicinal plant that is widely used in Asian countries. Its seed plays an important role in the treatment of many diseases because it contains various anthraquinones and flavonoids. Our previous studies have indicated that three space environment-induced S. obtusifolia lines (SP-lines) i.e., QC10, QC29, and QC46, have higher seed yield and aurantio-obtusin (AO) content. However, the underlying mechanism of higher AO content in SP-lines is still unknown. Herein, transcriptome sequencing and HPLC were employed to analyze the differences between SP-lines and ground control (GC3) and elucidate the regulatory mechanisms of AO accumulation in SP-lines. The results show that 4002 differentially expressed genes (DEGs) were identified in SP-lines versus (vs.) GC3. DEGs in the QC10 vs. GC3, QC29 vs. GC3, and QC46 vs. GC3 comparisons were classified into 28, 36, and 81 GO terms and involved in 63, 74, and 107 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. KEGG pathway and gene expression analysis revealed that DEGs involved in anthraquinone pathways were significantly elevated in QC10 and QC46. Integrating the results of GO annotation, KEGG enrichment, and gene expression analysis, we propose that the elevated genes such as DAHPS, DHQS, and MenB enhance the metabolic flux in the anthraquinone pathway and promote AO content in QC10 and QC46. Taken together, this study elucidated the mechanism of AO content in SP-lines and provides valuable genetic information for S. obtusifolia. In addition, to the best of our knowledge, this study presents the first transcriptome analysis of environment-induced medicinal plants and paves the way to select elite S. obtusifolia varieties in the future.

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“…Shoots of seedlings were harvested to perform enzyme extraction and assays. The enzyme activity of SOD, POD, CAT, and APX was determined, as previously described ( Mao et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Exogenous tryptophan application improves cadmium tolerance and inhibits cadmium upward transport in broccoli (Brassica oleracea var. italica)

et al. 2022

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Cadmium (Cd) pollution not only reduces crop yields, but also threatens human health and food safety. It is of great significance for agricultural production to improve plant Cd resistance and reduce Cd accumulation. In Arabidopsis, tryptophan (Trp) has been found to play a role in Cd resistance. However, studies on the role of exogenous Trp on Cd tolerance in crops are limited. Here, we report that exogenous Trp application can effectively alleviate biomass decline induced by Cd stress and inhibit Cd transport from roots to shoots in Brassica oleracea var. italica (broccoli). Compared to Cd stress alone, the fresh weight of shoots and roots of B. oleracea seedlings treated with Cd and Trp increased by 25 and 120%, respectively, and the Cd content in shoots decreased by 51.6%. In combination with physiological indices and transcriptome analysis, we preliminarily explored the mechanism of Trp alleviating Cd stress and affecting Cd transport. Trp inhibited Cd-induced indole-3-acetic acid (IAA) conjugation, thereby providing enough free IAA to sustain growth under Cd stress; Trp inhibited the indolic glucosinolate (IGS) biosynthesis induced by Cd. Considering that the synthesis of IGS consumes glutathione (GSH) as a sulfur donor, the inhibition of Trp in IGS synthesis may be conducive to maintaining a high GSH content to be against Cd stress. Consistent with this, we found that GSH content under Cd stress with Trp application was higher than that of Cd alone. In addition to alleviating the damage caused by Cd, Trp can also inhibit the upward transport of Cd from roots to shoots, possibly by repressing the expression of HMA4, which encodes a transporter responsible for the xylem loading and Cd upward transport.

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Physiological, transcriptional, and metabolic alterations in spaceflight-subjected Senna obtusifolia

Cited by 8 publications

References 31 publications

Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

Examination of the Transcriptional Response to LaMIR166a Overexpression in Larix kaempferi (Lamb.) Carr

Transcriptome and HPLC Analysis Reveal the Regulatory Mechanisms of Aurantio-Obtusin in Space Environment-Induced Senna obtusifolia Lines

Exogenous tryptophan application improves cadmium tolerance and inhibits cadmium upward transport in broccoli (Brassica oleracea var. italica)

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