Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation

Camasão, Dimitria Bonizol; Li, Ling; Drouin, Bernard; Lau, Cori; Reinhardt, Dieter P.

doi:10.1007/s44164-022-00012-1

Cited by 3 publications

(2 citation statements)

References 64 publications

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“…Furthermore, the construct was cultured under physiological mechanical stimuli. This method proved to improve both cell alignment, remodeling, and EMC production, along with the scaffold’s viscoelastic properties, when compared to grafts matured in static conditions, thus producing a reliable in vitro model of the vascular wall [ 107 ]. In another study, Bosch-Rué et al prepared bilayered cellularized collagen TEVGs with a coaxial extrusion method.…”

Section: Natural Biomaterials For Vascular Tissue Engineeringmentioning

confidence: 99%

Biological Materials for Tissue-Engineered Vascular Grafts: Overview of Recent Advancements

Di Francesco,

Pigliafreddo,

Casarella

et al. 2023

Biomolecules

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The clinical demand for tissue-engineered vascular grafts is still rising, and there are many challenges that need to be overcome, in particular, to obtain functional small-diameter grafts. The many advances made in cell culture, biomaterials, manufacturing techniques, and tissue engineering methods have led to various promising solutions for vascular graft production, with available options able to recapitulate both biological and mechanical properties of native blood vessels. Due to the rising interest in materials with bioactive potentials, materials from natural sources have also recently gained more attention for vascular tissue engineering, and new strategies have been developed to solve the disadvantages related to their use. In this review, the progress made in tissue-engineered vascular graft production is discussed. We highlight, in particular, the use of natural materials as scaffolds for vascular tissue engineering.

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Section: Natural Biomaterials For Vascular Tissue Engineeringmentioning

confidence: 99%

Biological Materials for Tissue-Engineered Vascular Grafts: Overview of Recent Advancements

Di Francesco,

Pigliafreddo,

Casarella

et al. 2023

Biomolecules

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“…These collagen-based models ( Bono et al, 2016 ; Loy et al, 2017 ; Camasão et al, 2018 ; Loy et al, 2018 ; Camasão et al, 2020 ; González-Pérez et al, 2021 ) showed promising results in mimicking the biological properties of a native vessel but lacked appropriate mechanical properties. More specifically, (non-reinforced) collagen-based models were unable to withstand the high pressures and stresses encountered in the blood vessels ( Gaudet and Shreiber, 2012 ; Copes et al, 2019 ; Camasão et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Polymeric reinforcements for cellularized collagen-based vascular wall models: influence of the scaffold architecture on the mechanical and biological properties

Pien,

Di Francesco,

Copes

et al. 2023

Front. Bioeng. Biotechnol.

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A previously developed cellularized collagen-based vascular wall model showed promising results in mimicking the biological properties of a native vessel but lacked appropriate mechanical properties. In this work, we aim to improve this collagen-based model by reinforcing it using a tubular polymeric (reinforcement) scaffold. The polymeric reinforcements were fabricated exploiting commercial poly (ε-caprolactone) (PCL), a polymer already used to fabricate other FDA-approved and commercially available devices serving medical applications, through 1) solution electrospinning (SES), 2) 3D printing (3DP) and 3) melt electrowriting (MEW). The non-reinforced cellularized collagen-based model was used as a reference (COL). The effect of the scaffold’s architecture on the resulting mechanical and biological properties of the reinforced collagen-based model were evaluated. SEM imaging showed the differences in scaffolds’ architecture (fiber alignment, fiber diameter and pore size) at both the micro- and the macrolevel. The polymeric scaffold led to significantly improved mechanical properties for the reinforced collagen-based model (initial elastic moduli of 382.05 ± 132.01 kPa, 100.59 ± 31.15 kPa and 245.78 ± 33.54 kPa, respectively for SES, 3DP and MEW at day 7 of maturation) compared to the non-reinforced collagen-based model (16.63 ± 5.69 kPa). Moreover, on day 7, the developed collagen gels showed stresses (for strains between 20% and 55%) in the range of [5–15] kPa for COL, [80–350] kPa for SES, [20–70] kPa for 3DP and [100–190] kPa for MEW. In addition to the effect on the resulting mechanical properties, the polymeric tubes’ architecture influenced cell behavior, in terms of proliferation and attachment, along with collagen gel compaction and extracellular matrix protein expression. The MEW reinforcement resulted in a collagen gel compaction similar to the COL reference, whereas 3DP and SES led to thinner and longer collagen gels. Overall, it can be concluded that 1) the selected processing technique influences the scaffolds’ architecture, which in turn influences the resulting mechanical and biological properties, and 2) the incorporation of a polymeric reinforcement leads to mechanical properties closely matching those of native arteries.

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Toward In Vitro Vascular Wall Models: Digital Light Processing of Acrylate‐Endcapped Urethane‐Based Polymers into Tubular Constructs

Pien,

Pokholenko,

Deroose

et al. 2024

Macro Chemistry & Physics

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Digital light processing (DLP) is emerging as a powerful tool for fabricating tissue engineering (TE) scaffolds, particularly for vascular TE and the development of representative in vitro vascular wall models. For the latter, biomaterials should mimick the biological and mechanical properties of native blood vessels. To fabricate tubular constructs, the DLP‐printing process is optimized by exploiting acrylate‐endcapped urethane‐based (AUP) polymers as the presence of the acrylate end groups render them suitable for DLP printing and desirable mechanical properties arise from the urethane segments. Four AUP variants are synthesized, exploring polyethylene glycol (PEG) and polypropylene glycol (PPG) backbones with varying acrylate functionalities (di‐acrylate versus hexa‐acrylate), namely UPEG2, UPEG6, UPPG2, and UPPG6. Tubular constructs with precise dimensions and morphology are fabricated. PPG‐based AUP polymers exhibit superior computer‐aided design/manufacturing (CAD/CAM) mimicry compared to PEG‐based derivatives. Construct characterization reveals tunable mechanical properties, with elastic moduli ranging from 45 to 259 kPa, reaching values of the human blood vessels. In particular, UPPG6 shows a two‐fold higher elastic modulus compared to UPPG2. All materials show excellent biocompatibility. Additionally, surface modification with gelatin‐methacryloyl (GELMA) significantly enhances the cytocompatibility of UPPG2 scaffolds. This study demonstrates the feasibility of fabricating tubular constructs with tunable properties using DLP and AUP polymers.

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Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation

Cited by 3 publications

References 64 publications

Biological Materials for Tissue-Engineered Vascular Grafts: Overview of Recent Advancements

Biological Materials for Tissue-Engineered Vascular Grafts: Overview of Recent Advancements

Polymeric reinforcements for cellularized collagen-based vascular wall models: influence of the scaffold architecture on the mechanical and biological properties

Toward In Vitro Vascular Wall Models: Digital Light Processing of Acrylate‐Endcapped Urethane‐Based Polymers into Tubular Constructs

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