Due to recent classification as a commodity crop in North America, producer interest in industrial hemp (Cannabis sativa L.) has increased. In the Commonwealth of Virginia, there is a need to evaluate foreign-developed industrial hemp for its adaptability and to develop new cultivars suited to local climatic conditions. Eight cultivars (‘Bialobrzeskie’, ‘Canda’, ‘Fedora 17’, ‘Felina 32’, ‘Joey’, ‘Tygra’, ‘USO 31’, and ‘Wojko’) were evaluated for grain and five (‘Bialobrzeskie’, ‘Carmagnola’, ‘Fedora 17’, ‘Futura 75’, and ‘Wojko’) for vegetative biomass. An experiment carried out at the Virginia State Research and Demonstration (Randolph) farm was laid out in a randomized complete block design with three replications. The results show that cultivars differed in the cumulative growing degree days (CGDD) needed for growth phase transitions, with ‘USO 31’ having an early transition to the reproductive phase. In addition, ‘Fedora 17’ produced greater grain yield and may have the potential for greater performance with better management to improve its adaptability to local conditions. Across cultivars and years, the grain protein content averaged 241 g kg−1 and the mineral elements were at sufficient levels for animal and human nutrition. ‘Carmagnola’ produced greater biomass yield and has the potential for adoption as local fiber cultivar.