Physiology and Pathophysiology of Selectins, Integrins, and IgSf Cell Adhesion Molecules Focusing on Inflammation. A Paradigm Model on Infectious Endocarditis

Golias, Christos; Batistatou, Anna; Bablekos, Georgios; Charalabopoulos, Alexandros; Peschos, Dimitrios; Mitsopoulos, Panagiotis; Charalabopoulos, Konstantinos

doi:10.3109/15419061.2011.606381

Cited by 51 publications

(42 citation statements)

References 94 publications

(110 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to atherosclerosis, adhesion molecules such as ICAM-1 are also implicated in the progression of infection (35)(36)(37). A recent study reported that manganese blocks intracellular trafficking of Shiga toxin and that manganese-supplemented mice were completely resistant to a lethal Shiga toxin challenge (38).…”

Section: Discussionmentioning

confidence: 99%

Manganese Supplementation Reduces High Glucose-induced Monocyte Adhesion to Endothelial Cells and Endothelial Dysfunction in Zucker Diabetic Fatty Rats

Burlet

Jain

2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:Mn 2ϩ levels are lower in blood of diabetic and atherosclerosis patients. Results: Mn 2ϩ supplementation reduces monocyte adhesion in endothelial cells by down-regulating ROS, ICAM-1 expression, and MCP-1 secretion, and lowers blood levels of ICAM-1 and cholesterol in ZDF rats. Conclusion:Mn 2ϩ supplementation is beneficial in lowering markers of endothelial dysfunction. Significance: Mn 2ϩ supplementation can potentially prevent or delay progression of atherosclerosis.Endothelial dysfunction is a hallmark of increased vascular inflammation, dyslipidemia, and the development of atherosclerosis in diabetes. Previous studies have reported lower levels of Mn 2؉ in the plasma and lymphocytes of diabetic patients and in the heart and aortic tissue of patients with atherosclerosis. This study examines the hypothesis that Mn 2؉ supplementation can reduce the markers/risk factors of endothelial dysfunction in type 2 diabetes. Human umbilical vein endothelial cells (HUVECs) were cultured with or without Mn 2؉ supplementation and then exposed to high glucose (HG, 25 mM) to mimic diabetic conditions. Mn 2؉ supplementation caused a reduction in monocyte adhesion to HUVECs treated with HG or MCP-1. Mn 2؉ also inhibited ROS levels, MCP-1 secretion, and ICAM-1 up-regulation in HUVECs treated with HG. Silencing studies using siRNA against MnSOD showed that similar results were observed in MnSOD knockdown HUVECs following Mn 2؉ supplementation, suggesting that the effect of manganese on monocyte adhesion to endothelial cells is mediated by ROS and ICAM-1, but not MnSOD. To validate the relevance of our findings in vivo, Zucker diabetic fatty rats were gavaged daily with water (placebo) or MnCl 2 (16 mg/kg of body weight) for 7 weeks. When compared with placebo, Mn 2؉ -supplemented rats showed lower blood levels of ICAM-1 (17%, p < 0.04), cholesterol (25%, p < 0.05), and MCP-1 (28%, p ‫؍‬ 0.25). These in vitro and in vivo studies demonstrate that Mn 2؉ supplementation can down-regulate ICAM-1 expression and ROS independently of MnSOD, leading to a decrease in monocyte adhesion to endothelial cells, and therefore can lower the risk of endothelial dysfunction in diabetes.Manganese is an essential micronutrient that serves as a cofactor for many enzyme systems. Metalloenzymes, or manganese-containing enzymes, such as arginase, pyruvate carboxylase, and manganese superoxide dismutase (MnSOD), 2 require Mn 2ϩ to function. MnSOD is the major mitochondrial antioxidant and is responsible for protecting the cell from reactive oxygen species (ROS) by scavenging mitochondrial superoxide (1). MnSOD acts by catalyzing the conversion of superoxide radicals (such as O 2 ) to hydrogen peroxide, which is further metabolized to water by other antioxidant enzymes such as catalase and glutathione peroxidase (2). At low concentrations, Mn 2ϩ ions have been shown to have antioxidant properties with the ability to scavenge superoxide and hydroxyl radicals (3). Several studies have reported that changes in dietary Mn 2ϩ induced changes in MnS...

show abstract

Section: Discussionmentioning

confidence: 99%

Manganese Supplementation Reduces High Glucose-induced Monocyte Adhesion to Endothelial Cells and Endothelial Dysfunction in Zucker Diabetic Fatty Rats

Burlet

Jain

2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Integrins are transmembrane receptors that are the bridges for cellcell and cell-extracellular matrix interactions with other receptors such as cadherins, the immunoglobulin superfamily cell adhesion molecules [22]. The ITGB3 protein may combine with multiple partners resulting in different integrins.…”

Section: Differential Proteome and Extracellular Matrix (Ecm) In Siasmentioning

confidence: 99%

Comparative proteome analysis of saccular intracranial aneurysms with iTRAQ quantitative proteomics

Jia

Huang

et al. 2016

Journal of Proteomics

View full text Add to dashboard Cite

“…This is a complex and multi-mediated process that results from the release of inflammatory mediators and involves proteins with lectin domains, the selectins [20]. These proteins, which are present both in neutrophil and endothelial cells, interact with their respective carbohydrate ligands mediating the process called rolling.…”

Section: Introductionmentioning

confidence: 99%

Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides

et al. 2014

View full text Add to dashboard Cite

Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(α1-3)Man(α1-O)Me, Man(α1-4)Man(α1-O)Me and 5-bromo-4-chloro-3-indolyl-α-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.

show abstract

Physiology and Pathophysiology of Selectins, Integrins, and IgSf Cell Adhesion Molecules Focusing on Inflammation. A Paradigm Model on Infectious Endocarditis

Cited by 51 publications

References 94 publications

Manganese Supplementation Reduces High Glucose-induced Monocyte Adhesion to Endothelial Cells and Endothelial Dysfunction in Zucker Diabetic Fatty Rats

Manganese Supplementation Reduces High Glucose-induced Monocyte Adhesion to Endothelial Cells and Endothelial Dysfunction in Zucker Diabetic Fatty Rats

Comparative proteome analysis of saccular intracranial aneurysms with iTRAQ quantitative proteomics

Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides

Contact Info

Product

Resources

About