Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Zhang, Sheng; Zhang, Lingling; Chai, Yongyu; Wang, Fei; Li, Yiming; Су, Ли; Zhong, Zhao

doi:10.1016/j.jprot.2015.06.019

Cited by 29 publications

(28 citation statements)

References 111 publications

(123 reference statements)

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“…In this study, MDA content increased in leaves of ancient P. orientalis, whereas chlorophyll levels and SOD and POD activity declined (Figure 11), which is consistent with an earlier study of ancient pine [4]. These findings indicate that the ROS scavenging capabilities of ancient trees are weakened, resulting in ROS accumulation in ancient trees during aging [3]. As shown in Figure 10b, the leaves of 20-year-old trees have the highest carotenoid content, followed by 700-, 1100-and 200-year-old trees.…”

Section: Discussionsupporting

confidence: 91%

“…Protein and ROS levels are often used as markers for the progression of senescence [3]. The soluble protein level was high in 20-year-old samples, and then gradually decreased to a minimum in 700-year-old samples ( Figure 2).…”

Section: Biochemical Changes During Senescencementioning

confidence: 99%

“…Several woody perennials, including Sequoia sempervirens, Sequoiadendron giganteum, Platycladus orientalis, and Pinus longaeva, can live for several hundred years or even millennia [1,2]. Plant biologists are currently interested in the physiological and molecular mechanisms mediating or preventing senescence [3,4]. Some physiological and metabolic changes during aging have been reported in conifers [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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De novo Characterization of the Platycladus orientalis Transcriptome and Analysis of Photosynthesis-Related Genes during Aging

Chang

Zhang

Yao

et al. 2019

Forests

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In China, Platycladus orientalis has a lifespan of thousands of years. The long lifespan of these trees may be relevant for the characterization of plant aging at the molecular level. However, the molecular mechanism of the aging process of P. orientalis is still unknown. To explore the relationship between age and growth of P. orientalis, we analyzed physiological changes during P. orientalis senescence. The malondialdehyde content was greater in 200-, 700-, and 1100-year-old ancient trees than in 20-year-old trees, whereas the peroxidase and superoxide dismutase activities, as well as the soluble protein content, exhibited the opposite trend. Furthermore, we performed a de novo transcriptome assembly using RNA-Seq and obtained 48,044 unigenes with an average length of 896 bp. A total of 418 differentially expressed genes were identified in different stages of aging of P. orientalis. Clustering analysis revealed distinct timepoints at which the oxidation–reduction and photosynthesis pathways changed. Eight clusters with distinct expression patterns were identified. The expression levels of photosynthesis-, oxidation–reduction-, and transporter-related genes were down-regulated, whereas those of transcription-, signaling-, and senescence-related genes were up-regulated during aging. In addition, consistent with the most obviously down-regulated genes of photosynthesis-related genes, the photosynthetic indexes including chlorophyll a and b levels decreased steadily during P. orientalis aging. This study combined transcriptome with physiological and biochemical data, revealing potential candidate genes influencing senescence during P. orientalis aging.

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Section: Discussionsupporting

confidence: 91%

Section: Biochemical Changes During Senescencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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De novo Characterization of the Platycladus orientalis Transcriptome and Analysis of Photosynthesis-Related Genes during Aging

Chang

Zhang

Yao

et al. 2019

Forests

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“…These ndings suggest that protein kinase and calmodulin functioned as positive regulators to improve the heat tolerance of HT seedlings. In addition, 14-3-3 proteins were also identi ed as cold/salt/drought-responsive proteins, respectively [22,27,28]. Moreover, 14-3-3 protein 4, 14-3-3 protein 9, and 14-3-3 protein 10 were more accumulated in HT compared with HS under heat stress.…”

Section: Daps Involved In Signal Transductionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

Wang

Liang

Yang

et al. 2020

Preprint

Self Cite

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Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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“…They are associated primarily with chlorophylls and xanthophylls, which serve as antenna complexes that absorb sunlight and transfer the excitation energy to the core complexes of photosystem II to drive photosynthetic electron transport [26,27].…”

Section: Proteins Related To Carbohydrate and Energy Metabolismmentioning

confidence: 99%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

Tang

Cai

et al. 2020

Preprint

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5℃ for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

Cited by 29 publications

References 111 publications

De novo Characterization of the Platycladus orientalis Transcriptome and Analysis of Photosynthesis-Related Genes during Aging

De novo Characterization of the Platycladus orientalis Transcriptome and Analysis of Photosynthesis-Related Genes during Aging

iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

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