Physiology of anammox adaptation to low temperatures and promising biomarkers: A review

Kouba, Vojtěch; Bachmannová, Ch.; Podzimek, T.; Lipovová, Petra; Loosdrecht, Mark C.M. van

doi:10.1016/j.biortech.2022.126847

Cited by 46 publications

(13 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As mentioned above, anammox bacteria are not available for pure culture to date; thus, molecular biology methods based on monoclones cannot be used for the identification of metabolic pathways or bioaugmentation. Many questions about the basic metabolic pathways of anammox bacteria have not yet been addressed (Peeters and van Niftrik, 2019; Kuenen, 2020), challenges (such as the fast start-up and operation under non-optimal conditions) during the full-scale operation of anammox also require attention (Adams et al, 2020; Kouba et al, 2022), and this will require the development of methods for the genome editing of anammox bacteria. We believe that the transposon containing tnpA might be one of the breakthroughs for the genome editing of anammox bacteria in mixed cultures.…”

Section: Discussionmentioning

confidence: 99%

New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

Wang,

Mao,

et al. 2023

Preprint

View full text Add to dashboard Cite

Anaerobic ammonium-oxidation (anammox) bacteria play a crucial role in global nitrogen cycling and wastewater nitrogen removal, but they depend on mutualistic symbiosis with various other microorganisms for their survival. Although shotgun metagenomics based on short reads has been widely used in anammox research, metagenome-assembled genomes (MAGs) are often discontinuous and highly contaminated, which limits in-depth analyses of anammox communities. Here, for the first time, we performed Pacific Biosciences high-fidelity (HiFi) sequencing on the anammox granule sludge sample, and obtained 30 accurate and complete metagenome-assembled genomes (cMAGs). These cMAGs were obtained by selecting high-quality circular contigs from initial assemblies of long reads generated by HiFi sequencing, eliminating the need for Illumina short reads, binning, and reassembly. cMAG-centric analysis revealed divergences in general and nitrogen metabolism among members of the anammox community. Furthermore, we identified mobile genetic elements (MGEs) and putative horizontal gene transfer (HGT) events within these cMAGs to explore the adaptive evolution of the community. The results suggest that MGEs and HGT events, particularly transposons containing tnpA in anammox bacteria, might play important roles in the adaptive evolution of this anammox community. The cMAGs generated in the present study could be used to establish of a comprehensive database for anammox bacteria and associated microorganisms. Our findings highlight the advantages of HiFi sequencing for the studies of complex cultures such as anammox communities and advance our understanding of anammox communities.

show abstract

Section: Discussionmentioning

confidence: 99%

New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

Wang,

Mao,

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The diffuse spectral reflectance (530–520 nm) was 0.55 ± 0.05, indicating that the gray, fine sandy sediments were under weak-reducing conditions . Sediment subsamples for microcosms were wrapped tightly with aluminum foil immediately after collection onsite, sealed in sterile plastic bags filled with ultrapure N 2 (>99.999%), and transported to the laboratory using ice and stored at −20 °C until incubations . Sediment subsamples for DNA analysis were transported to the laboratory using dry ice and then kept frozen at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Ammonium-Enhanced Arsenic Mobilization from Aquifer Sediments

Xiu,

Gai,

Chen

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

Ammonium-related pathways are important for groundwater arsenic (As) enrichment, especially via microbial Fe(III) reduction coupled with anaerobic ammonium oxidation; however, the key pathways (and microorganisms) underpinning ammonium-induced Fe(III) reduction and their contributions to As mobilization in groundwater are still unknown. To address this gap, aquifer sediments hosting high As groundwater from the western Hetao Basin were incubated with 15 N-labeled ammonium and external organic carbon sources (including glucose, lactate, and lactate/acetate). Decreases in ammonium concentrations were positively correlated with increases in the total produced Fe(II) (Fe(II) tot ) and released As. The molar ratios of Fe(II) tot to oxidized ammonium ranged from 3.1 to 3.7 for all incubations, and the δ 15 N values of N 2 from the headspace increased in 15 N-labeled ammonium-treated series, suggesting N 2 as the key end product of ammonium oxidation. The addition of ammonium increased the As release by 16.1% to 49.6%, which was more pronounced when copresented with organic electron donors. Genome-resolved metagenomic analyses (326 good-quality MAGs) suggested that ammonium-induced Fe(III) reduction in this system required syntrophic metabolic interactions between bacterial Fe(III) reduction and archaeal ammonium oxidation. The current results highlight the significance of syntrophic ammonium-stimulated Fe(III) reduction in driving As mobilization, which is underestimated in high As groundwater.

show abstract

“…One of the main limitations of the wide application of anammox technology for wastewater treatment and bioremediation is the low ambient temperature [ 7 , 8 , 9 ]. This is especially true for groundwater ecosystems, where the year-round temperature usually does not exceed 10 °C, given that most anammox bacteria exploited for wastewater treatment have a temperature optimum of 30–40 °C [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…It was shown that the specific activity of anammox bacteria can drop dramatically (up to ten times) when the temperature declines from 30 to 10 °C [ 12 ], while a sharper activity decline was observed at 15 °C [ 12 , 13 ]. However, numerous works confirm the possibility of the gradual adaptation of the “warm” anammox community to lower temperatures [ 9 ]. Moreover, it has been reported that the optimum temperature for anammox activity in natural habitats may be as low as 9 °C in permeable Arctic sediment [ 14 ], 12 °C in the arctic, permanently cold marine sediments [ 15 ]; or 15 °C in marine sediment from Skaggerak (North Sea) [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Physiologically, the low temperature can reduce enzyme activity and the fluidity of bacterial membranes, alter the transport of nutrients and waste products, decrease the rates of transcription, translation, and cell division, denature proteins, fold proteins inappropriately, and form intracellular ice [ 17 ]. Thus, the price of adaptation to cold is the conservation of energy for the core metabolism due to a decrease in biosynthesis in general, leading to reduced growth rate and activity of anammox bacteria [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Mineral Carriers on Biofilm Formation and Nitrogen Removal Activity by an Indigenous Anammox Community from Cold Groundwater Ecosystem Alone and Bioaugmented with Biomass from a “Warm” Anammox Reactor

Vishnyakova

Popova

Artemiev

et al. 2022

Biology

View full text Add to dashboard Cite

The complex pollution of aquifers by reduced and oxidized nitrogen compounds is currently considered one of the urgent environmental problems that require non-standard solutions. This work was a laboratory-scale trial to show the feasibility of using various mineral carriers to create a permeable in situ barrier in cold (10 °C) aquifers with extremely high nitrogen pollution and inhabited by the Candidatus Scalindua-dominated indigenous anammox community. It has been established that for the removal of ammonium and nitrite in situ due to the predominant contribution of the anammox process, quartz, kaolin clays of the Kantatsky and Kamalinsky deposits, bentonite clay of the Berezovsky deposit, and zeolite of the Kholinsky deposit can be used as components of the permeable barrier. Biofouling of natural loams from a contaminated aquifer can also occur under favorable conditions. It has been suggested that the anammox activity is determined by a number of factors, including the presence of the essential trace elements in the carrier and the surface morphology. However, one of the most important factors is competition with other microbial groups that can develop on the surface of the carrier at a faster rate. For this reason, carriers with a high specific surface area and containing the necessary microelements were overgrown with the most rapidly growing microorganisms. Bioaugmentation with a “warm” anammox community from a laboratory reactor dominated by Ca. Kuenenia improved nitrogen removal rates and biofilm formation on most of the mineral carriers, including bentonite clay of the Dinozavrovoye deposit, as well as loamy rock and zeolite-containing tripoli, in addition to carriers that perform best with the indigenous anammox community. The feasibility of coupled partial denitrification–anammox and the adaptation of a “warm” anammox community to low temperatures and hazardous components contained in polluted groundwater prior to bioaugmentation should be the scope of future research to enhance the anammox process in cold, nitrate-rich aquifers.

show abstract

Physiology of anammox adaptation to low temperatures and promising biomarkers: A review

Cited by 46 publications

References 100 publications

New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

Ammonium-Enhanced Arsenic Mobilization from Aquifer Sediments

Effect of Mineral Carriers on Biofilm Formation and Nitrogen Removal Activity by an Indigenous Anammox Community from Cold Groundwater Ecosystem Alone and Bioaugmented with Biomass from a “Warm” Anammox Reactor

Contact Info

Product

Resources

About