Physiology of blood coagulation: Advances, problems and present status

VII. Ac-Globulin (Factor V)

“…The first time it was possible to obtain quantitative data on these interrelationships we used prethrombin 1, crude cephalin, and Ac-globulin concentrate free of other coagulation factors. Prethrombin 1, and Factor Xa were purified single component systems (Baker and Seegers 1967;Seegers 1968). We made the following combinations: ‡This specimen of plasma was obtained after 300 ml of whole blood had been infused.…”

“…Initial velocity recorded. There was a low yield of thrombin even though Factor Xa (Auto-C) concentration was higher than needed if Factor V had also been added (Seegers 1977).…”

“…Only phospholipid was left out of the five-component system. Ac-globulin increased the yield of thrombin, which was only 25% of the possible yield even with Factor V concentration at a level adequate in the five-component system (Seegers 1977). kg body weight, administered to a dog was followed by a prompt rise in plasma Factor V concentration that reached its peak on day three (Seegers 1951).…”

IX. Antithrombin III

“…Even with our expanded knowledge (Teng and Seegers 1981), there is no way to specify which structure was isolated. The main point now is that it was inactivated by purified At-III (Seegers, Schröer, and Kagami 1964;Seegers 1968). Other coverage of some of these facts was written (Seegers , 1967a(Seegers , 1975(Seegers , 1978.…”

XIV. Prothrombinase Complex

“…Information on both was essential. We set up the following experimental pro jections with purified reagents: (Baker and Seegers 1967;Seegers, McCoy and Marciniak 1968;Seegers 1968) it was concluded that any one of the variables could be re duced in concentration and be associated with a re duction in thrombin yield (Fig. 1).…”