Physiology of Camptothecin Synthesis in Plants and Root Organ Cultures of Ophiorrhiza mungos L. and Its Production in Root Fermenters

Hummel, Eric; Walczak, Steffen; Distl, Melanie; Langhans, Markus; Wetterauer, Bernhard; Sporer, Frank; Wildi, Eckhart; Wink, Michaël

doi:10.1007/978-3-030-58271-5_11

Cited by 2 publications

(1 citation statement)

References 44 publications

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“…prostrata (D.Don) Deb & Mondal Rubiaceae Stem 0.08% (Gharpure et al 2010 ) Root 0.16% Fruit 0.0165% Young leaves 0.0062% Mature leaves 0.0022% Ophiorrhiza filistipula Miq. Rubiaceae Leaves 0.00009% (Arbain et al 1993 ) Ophiorrhiza mungos L. Rubiaceae Root 0.0176% (Roja 2006 ) Shoot 0.0096% Young leaves 1664 µg g −1 (Wetterauer et al 2021 ) Mature leaves 2000 µg g −1 Ophiorrhiza mungos L. var. angustifolia (Thw.)…”

Section: Camptothecin: Structure and Its Derivatives/analoguesmentioning

confidence: 99%

Biotechnological approaches for the production of camptothecin

Banadka,

Narasimha,

Dandin

et al. 2024

Appl Microbiol Biotechnol

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Camptothecin (CPT), an indole alkaloid popular for its anticancer property, is considered the third most promising drug after taxol and famous alkaloids from Vinca for the treatment of cancer in humans. Camptothecin was first identified in Camptotheca acuminata followed by several other plant species and endophytic fungi. Increased harvesting driven by rising global demand is depleting the availability of elite plant genotypes, such as Camptotheca acuminata and Nothapodytes nimmoniana, crucial for producing alkaloids used in treating diseases like cancer. Conservation of these genotypes for the future is imperative. Therefore, research on different plant tissue culture techniques such as cell suspension culture, hairy roots, adventitious root culture, elicitation strategies, and endophytic fungi has been adopted for the production of CPT to meet the increasing demand without affecting the source plant’s existence. Currently, another strategy to increase camptothecin yield by genetic manipulation is underway. The present review discusses the plants and endophytes that are employed for camptothecin production and throws light on the plant tissue culture techniques for the regeneration of plants, callus culture, and selection of cell lines for the highest camptothecin production. The review further explains the simple, accurate, and cost-effective extraction and quantification methods. There is enormous potential for the sustainable production of CPT which could be met by culturing of suitable endophytes or plant cell or organ culture in a bioreactor scale production. Also, different gene editing tools provide opportunities for engineering the biosynthetic pathway of CPT, and the overall CPT production can be improved . Key points • Camptothecin is a naturally occurring alkaloid with potent anticancer properties, primarily known for its ability to inhibit DNA topoisomerase I. • Plants and endophytes offer a potential approach for camptothecin production. • Biotechnology approaches like plant tissue culture techniques enhanced camptothecin production.

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