Physiology of Effects of Temporary Immersion Bioreactor (Tib) on Micropropagated Plantlets

Escalona, Maritza; Aragón, Carlos Alvareza; Capote, Iris; Pina, Danilo; Cejas, Inaudis; Rodríguez, R.; Cañal, M.J.; Sandoval, Jorge; Roels, Sophie; Debergh, Pierre; Desjardins, Yves; González-Olmedo, J. L.

doi:10.17660/actahortic.2007.748.9

Cited by 6 publications

(6 citation statements)

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“…From the results, it is evident that shoot bud proliferation rate is a function of meta-Topolin concentration. Similar results were also noticed in Williams banana [11], Ney Poovan banana [13], and in plantain [12] under In vitro conditions. Differences in sensitivity could be due to cultivardependent responses to the different cytokinin concentrations [1].…”

Section: Meta-topolin Concentration Wisesupporting

confidence: 84%

“…Application of a high concentration of mT evoked a higher degree of shoot proliferation in Kovvur Bontha than in the other two cultivars. The high cytokinin activity of mT was reported by several workers in banana [11,12].…”

Section: Subculture Wisesupporting

confidence: 55%

“…2c), where the maximum number of shoots per explant was produced at a concentration of 1.5 mT in all the subcultures, i.e., up to the sixth subculture (C6). Similarly, superior multiplication rates for mT treatments were reported in banana cultivars 'Williams' and 'Grand Naine' [11], in Patakpura banana [14], and in plantain [12].…”

Section: Cultivar Wise Proliferationmentioning

confidence: 58%

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Standardization of Meta-Topolin Concentration for Maximizing In vitro Proliferation in Commercial Banana Cultivars of Andhra Pradesh, India

Lakshmi,

Mamatha,

Bhagavan

et al. 2023

IJECC

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Cytokinins are associated with cell division, cell growth, and differentiation, are the most common plant growth regulators used in micropropagation. Aromatic cytokinin, meta-topolin (mT), and its derivatives have been demonstrated as alternative cytokinins in the in vitro multiplication of various plants. Different banana cultivars differ in in vitro shoot proliferation due to the difference in endogenous growth regulator content among them. A study was conducted to figure out the effect of different concentrations of meta-topolin on in vitro shoot proliferation of banana cultivars like Karpura chakkera keli (AAB), Mortoman (AAB), and Kovvur Bontha (ABB). In vitro shoot proliferation was initiated from the first subculture (C1) itself at 1.5 ppm mT concentration in Mortoman, Kovvur Bontha, and from the second subculture (C2) at 1.0 ppm mT concentration in Kovvur Bontha. Whereas, at all other remaining concentrations, shoot proliferation of different cultivars was initiated from the third subculture (C3) only. At the end of the sixth subculture (C6), the maximum number of shoots per explant (82.70) was recorded in the Kovvur Bontha cultivar at 1.5 ppm mT concentration. Among the cultivars, the Kovvur Bontha cultivar responded better to the meta-topolin for shoot proliferation than Karpura chakkera keli (AAB) and Mortoman (AAB).

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Section: Meta-topolin Concentration Wisesupporting

confidence: 84%

Section: Subculture Wisesupporting

confidence: 55%

Section: Cultivar Wise Proliferationmentioning

confidence: 58%

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Standardization of Meta-Topolin Concentration for Maximizing In vitro Proliferation in Commercial Banana Cultivars of Andhra Pradesh, India

Lakshmi,

Mamatha,

Bhagavan

et al. 2023

IJECC

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“…Positive effects on in vitro shoot multiplication with mT have been described with other species (Werbrouck et al 1996;Wojtania and Gabryszewska 2001;Bairu et al 2007Bairu et al , 2008Roels et al 2005). Negative carryover effects on rooting at too high mT levels (13.3 lM, 22.2 lM) have similarly been observed (Bairu et al 2008;Escalona et al 2003). Increased ex vitro acclimatization, particularly in EK 11-1, may be the consequence of a more rapid turnover of the mT main derivative N 6 -(3-O-b-D-glucopyranosyl)benzyladenine-9-riboside (Strnad et al 1997).…”

Section: Discussionmentioning

confidence: 81%

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

et al. 2009

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Benzyladenine (BA) is the only cytokinin to effectively induce shoot multiplication in vitro between genotypes of the important dune grass species Uniola paniculata (sea oats). However, a significant genotypespecific negative carryover effect of BA on ex vitro acclimatization has been observed. In the present study, the effects of multiplication media supplemented with metatopolin (mT), a BA-analog, BA or no plant growth regulator, were compared on in vitro multiplication, rooting and ex vitro acclimatization using easy-and difficult-to-acclimatize sea oats genotypes. Both genotypes exhibited similar in vitro shoot dry weight, number of harvestable shoots and percent rooting when cultured under standard conditions (with 2.2 lM BA) or with an equimolar concentration of mT. In addition, both genotypes exhibited similar ex vitro leaf length and shoot production under these two culture conditions. However, ex vitro acclimatization of rooted microcuttings of the difficult-to-acclimatize genotype significantly increased when produced on shoot multiplication medium containing mT rather than BA. Metatopolin concentrations 10 lM or greater were inhibitory to in vitro rooting and acclimatization ex vitro of both genotypes. Nevertheless, survival of the difficult-to-acclimatize genotype was significantly greater when cultured in the presence of 2.2 lM-30 lM mT, compared to 2.2 lM BA. Therefore, a potential solution to overcome the detrimental BA carryover effect on ex vitro survival in sea oats is the substitution of BA with 2.2 lM mT for Stage II shoot multiplication. Use of mT may provide an efficient method to ensure in vitro propagation of a large number of diverse sea oats genotypes for dune restoration.

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“…It is noteworthy that, although the content of photosynthetic pigments is closely related to the plant's ability to carry out photosynthesis through the capture and transposition of light energy, this does not mean that plants will necessarily perform better in the field. Other factors are extremely important for the full development of the plant organism, such as greater adaptation to the acclimatization of the transpiration route and the full availability of nutrients in in vitro cultivation, favored, among other things, by the renewal of the internal atmosphere in cultivation systems (Escalona et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Morphometric parameters and photosynthetic performance of in vitro propagated pineapple

Marchi

Ornellas²,

Fritshe³

et al. 2022

revagro

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Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was to evaluate the performance of continuous and temporary immersion systems in the micropropagation scale-up of the species. Shoots were obtained from explants subcultured in flasks with gelled culture medium and without gas exchange. The shoots were transferred to liquid MS medium supplemented with 2mM NAA, and 4mM BAP, and cultivated in four different devices: sealed flasks, flasks with semipermeable gas membranes, RITA®, and twin-flasks. After 45 days of cultivation, plant growth, fresh mass increment, the stomatal density of the abaxial surface of the leaves, the maximum quantum yield of the photosystem II, and contents of chlorophyll and carotenoids were analyzed. Significant differences were observed in plant growth, stomatal density, and contents of chlorophyll and carotenoids. The twin-flasks and RITA® devices revealed better results in morphological parameters, such as plant growth and stomatal density, while the treatments in sealed flasks and with membrane stood out in the chlorophyll content.

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Physiology of Effects of Temporary Immersion Bioreactor (Tib) on Micropropagated Plantlets

Cited by 6 publications

References 0 publications

Standardization of Meta-Topolin Concentration for Maximizing In vitro Proliferation in Commercial Banana Cultivars of Andhra Pradesh, India

Standardization of Meta-Topolin Concentration for Maximizing In vitro Proliferation in Commercial Banana Cultivars of Andhra Pradesh, India

Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes

Morphometric parameters and photosynthetic performance of in vitro propagated pineapple

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