Physiology of Growth at High Temperatures

Campbell, L. Leon; Pace, B.

doi:10.1111/j.1365-2672.1968.tb00338.x

Cited by 42 publications

(16 citation statements)

References 53 publications

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“…Correlations between the temperature at which heated bacteria start to die and the temperature of ribosome-associated DSC thermogram events have been observed (Campbell & Pace, 1968;Lepock et al, 1990;Mackey et at., 1993;MohacsiFarkas et al, 1994). This strongly suggests that the temperatures of ribosome-associated DSC events are an indication of ribosome stability, and also that ribosome damage may be an important factor causing cell death in cells undergoing physical stress.…”

Section: Discussionmentioning

confidence: 96%

“…(1991) partially characterized the main thermogram peaks obtained in E. coli using whole cells, the most prominent components being associated with ribosome denaturation. The maximum growth temperatures of different species of bacteria have been correlated with ribosome Tm values, suggesting a relationship between ribosome stability and thermal resistance (Campbell & Pace, 1968 ; Lepock et at., 1990;Mackey et al, 1993;Mohacsi-Farkas et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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The effects of hydrostatic pressure on ribosome conformation in Escherichia coli: an in vivo study using differential scanning calorimetry

1999

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Differential scanning calorimetry of whole Escherichia coli cells allowed the detection in wiwo of changes in ribosome conformation. This enabled for the first time an analysis of the effects of high hydrostatic pressures on ribosomes in living cells. A correlation was observed between loss of cell viability and decrease in ribosome-associated enthalpy in cells subjected to pressures of 50-250 MPa for 20 min. Cell death and ribosome damage were therefore closely related phenomena. In pressure-treated cells, the thermogram peak temperatures decreased, suggesting that the remaining ribosomes had adopted a less stable conformation. During subsequent incubation of the cultures at 37 "C, peak temperatures and enthalpies gradually increased over a period of 5 h. This change in ribosome conformation had no apparent effect on cell survival, as viability continued to decrease. The addition of 5 mM MgCI, before pressure treatment of cells prevented the reduction in stability of surviving ribosomes but had no effect on the initial loss of enthalpy or on cell viability.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The effects of hydrostatic pressure on ribosome conformation in Escherichia coli: an in vivo study using differential scanning calorimetry

1999

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“…The effect of temperature on the nutritional requirements of thermophilic species has also been reported. Campbell & Pace (1968) showed that folic acid is required for methyl- (Umbarger & Brown 1955). Leahy (1968) showed that the diauxic lag between growth on for the branched chain amino acids like valine, leucine and isoleucine.…”

Section: B Stearotherrnophilusmentioning

confidence: 99%

Defined minimal media for the growth of prototrophic and auxotrophic strains of Bacillus stearothermophilus

Lee

Brown

Cheung

1982

Journal of Applied Bacteriology

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Minimal chemically defined media for Bacillus stearothermophilus were developed at 60°C and quantitative requirements for each nutrient were determined. A prototrophic strain of B. stearothermophilus was grown in medium containing only glucose and mineral salts whereas auxotrophic strains in addition required biotin, thiamine, nicotinic acid and DL‐methionine. Metabolic interaction between L‐valine and L‐leucine was observed with auxotrophic organisms. The presence of L‐leucine in minimal medium necessitated the addition of L‐valine. Growth took place in the absence of both amino acids.

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“…Interest in micro-organisms living in extreme thermal environments has increased over the past few years (see, for example, Brock, 1967 ;Williams, 1975) and a number of enzymes from extreme thermophiles have been characterized (see, for example, Campbell & Pace, 1968;Singleton & Amelunxen, 1973;Zuber, 1976;Ljungdahl & Sherod, 1976). Despite interest in the membrane composition of such organisms (Ray et al, 1971 a, b;Jackson et al, 1973 a), little is known about their electron transport systems (Pask-Hughes & Williams, 1975;McFetters & Ulrich, 1972).…”

Section: Introductionmentioning

confidence: 99%

The Electron Transport System of an Extremely Thermophilic Bacterium

Hickey

Daniel

1979

Journal of General Microbiology

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Active, membrane-bound NADH and succinate oxidase activities with a temperature optimum of 75 "C were demonstrated in an extremely thermophilic bacterium. These were relatively stable in cell-free extracts and respiratory particles at 75 "C, but at 90 "C the half-lives of these oxidase systems were about 15 min in respiratory particles and 80 min in cell-free extracts. The stability of the NADH oxidase in respiratory particles at 90 "C was enhanced by 2 M-(NH,),SO,, 50% (v/v) glycerol and by NADH. A number of other substrates were oxidized by the electron transport system. Respiratory particles contained cytochromes a-613, a-602, b-559, cytochrome o and at least one c-type cytochrome, c-555. The soluble fraction contained at least two c-type cytochromes, at least one of which was CO-reactive. The sensitivity of NADH and succinate oxidases to a range of inhibitors was determined. I N T R O D U C T I O NInterest in micro-organisms living in extreme thermal environments has increased over the past few years (see, for example, Brock, 1967 ;Williams, 1975) and a number of enzymes from extreme thermophiles have been characterized (see, for example, Campbell & Pace, 1968;Singleton & Amelunxen, 1973;Zuber, 1976;Ljungdahl & Sherod, 1976). Despite interest in the membrane composition of such organisms (Ray et al., 1971 a, b;Jackson et al., 1973 a), little is known about their electron transport systems (Pask-Hughes & Williams, 1975;McFetters & Ulrich, 1972). In this paper we describe the electron transport system of a thermophilic bacterium isolated from the Rotorua thermal region of New Zealand. The organism was Thermus-like (H. Morgan, personal communication) and, pending the final results of taxonomic work, it will be referred to as Thermus T351. The electron transport system (this paper) and other physiological and biochemical activities (H. Morgan & D. A. Cowan, personal communication) displayed optimal activity between 70 and 80 "C and negligible activity below 40 "C. The organism can therefore be described as caldoactive (Heinen & Heinen, 1972 ;Williams, 1975) or extremely thermophilic. M E T H O D SOrganism. Thermus T351 was isolated from a hot pool (79 k 4 "C), low in sulphide, at pH 7.8. The organism grew well at 75 "C at pH 7.5 to 7.8, but grew poorly below 60 "C; it was obligately aerobic. Cells were Gram-negative, non-motile, non-sporulating rods.

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Physiology of Growth at High Temperatures

Cited by 42 publications

References 53 publications

The effects of hydrostatic pressure on ribosome conformation in Escherichia coli: an in vivo study using differential scanning calorimetry

The effects of hydrostatic pressure on ribosome conformation in Escherichia coli: an in vivo study using differential scanning calorimetry

Defined minimal media for the growth of prototrophic and auxotrophic strains of Bacillus stearothermophilus

The Electron Transport System of an Extremely Thermophilic Bacterium

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