2023
DOI: 10.1002/pmic.202200474
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Physiology of trans‐translation deficiency in Bacillus subtilis – a comparative proteomics study

Abstract: Abstracttrans‐Translation is the most effective ribosome rescue system known in bacteria. While it is essential in some bacteria, Bacillus subtilis possesses two additional alternative ribosome rescue mechanisms that require the proteins BrfA or RqcH. To investigate the physiology of trans‐translation deficiency in the model organism B. subtilis, we compared the proteomes of B. subtilis 168 and a ΔssrA mutant in the mid‐log phase using gel‐free label‐free quantitative proteomics. In chemically defined medium, … Show more

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