Physiolometrics and the puzzle of methodical acumen

Hartmann, Jacob Peter; Olsen, Markus Harboe; Rose, George; Reichelt, M.; Berg, Ronan M. G.

doi:10.1113/ep091406

Cited by 7 publications

(19 citation statements)

References 19 publications

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“…Calculating the coefficient of variation as another measure of relative reliability is therefore seen as beneficial for interpretation (Gomez & Gomez, 1984). Nevertheless, the CV is also not without its flaws, as when the mean is close to zero, inaccurate results can occur, which is likely the cause of the inflated CV values in the current study (Hartmann et al, 2023). As such, the addition of absolute reliability measurements via the Bland-Altman plot and calculation of the smallest real difference is required to provide a more comprehensive review of overall reliability.…”

Section: Discussionmentioning

confidence: 96%

“…pDCs have been shown to possess greater inflammatory and migratory potential compared to mDCs (Liu et al, 2021), so it may be unsurprising that previous studies have found a preferential mobilization of pDCs during exercise. As previously discussed, similarly to lymphocytes, the degree of DC mobilization during intense exercise is positively correlated with the concentration of catecholamines The ICC is commonly used as a measure of relative reliability, as it can conveniently categorise the reliability of a measure as poor, moderate, good or excellent (Hartmann et al, 2023;Koo & Li, 2016).…”

Section: Discussionmentioning

confidence: 98%

“…However, caution must be taken when utilizing ICC as a sole measure of reliability, as the high within-group standard deviation associated with a very heterogeneous population may lead to a high ICC value regardless of how unreliable the method is (Hartmann et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…The ICC is commonly used as a measure of relative reliability, as it can conveniently categorise the reliability of a measure as poor, moderate, good or excellent (Hartmann et al., 2023 ; Koo & Li, 2016 ). However, caution must be taken when utilizing ICC as a sole measure of reliability, as the high within‐group standard deviation associated with a very heterogeneous population may lead to a high ICC value regardless of how unreliable the method is (Hartmann et al., 2023 ). As such, it is argued that the ICC does not provide a comprehensive assessment of reliability when taken alone.…”

Section: Discussionmentioning

confidence: 99%

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The reproducibility of dendritic cell and T cell counts to a 30‐min high‐intensity cycling protocol as a tool to highlight overtraining

Baker,

Piasecki,

Hunt

et al. 2023

Experimental Physiology

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Heavy training has been reported to be immunosuppressive in athletes and lead to blunted cortisol responses to exercise. Cortisol elevates the number of dendritic cells (DCs), key antigen‐presenting cells that interact with T cells to initiate an immune response. Reproducible cortisol responses to a 30‐min cycle test have been identified but were based on percentage of work rate maximum. To ensure physiological consistency, submaximal anchors, that is, ventilatory threshold (VT1) should prescribe intensity. This study aims to assess the reproducibility of the DC and T cell responses to an adapted stress test to assess its usefulness in assessing DC dysfunction with intensified training. Twelve males cycled for 1 min at 20% below VT1 and 4 min at 50% between VT1 and , for 30 min (20/50), with blood samples pre‐, post‐ and 30 min post‐exercise. This was repeated twice, 2–7 days apart. Flow cytometry assessed total DCs, plasmacytoid DCs, myeloid DCs, total T cells, T helper cells and T cytotoxic cells. No significant trial or interaction effects were found for any variable. A significant main effect of time for all variables was found; immune cells increased from pre‐ to post‐exercise and decreased to baseline 30 min post‐exercise, apart from plasmacytoid DCs, which remained elevated 30 min post‐exercise. Intraclass correlation coefficients showed overall good‐to‐excellent reliability for all immune cells, with smallest real difference and Bland–Altman analysis verifying high reproducibility between trials. These results suggest that the 20/50 exercise test induces reproducible DC and T cell count changes, which, implemented before and after a period of intensified training, may highlight the negative states of overtraining.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The reproducibility of dendritic cell and T cell counts to a 30‐min high‐intensity cycling protocol as a tool to highlight overtraining

Baker,

Piasecki,

Hunt

et al. 2023

Experimental Physiology

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show abstract

“…indicating that it has a limited value as a biomarker (Hartmann et al, 2023;Olsen, Riberholt, Plovsing et al, 2022).…”

Section: Bias (Units) Srd (Units) CV (%) Icc (%)mentioning

confidence: 99%

Myths and methodologies: Assessment of dynamic cerebral autoregulation by the mean flow index

Olsen,

Riberholt,

Berg

et al. 2024

Experimental Physiology

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The mean flow index—usually referred to as Mx—has been used for assessing dynamic cerebral autoregulation (dCA) for almost 30 years. However, concerns have arisen regarding methodological consistency, construct and criterion validity, and test–retest reliability. Methodological nuances, such as choice of input (cerebral perfusion pressure, invasive or non‐invasive arterial pressure), pre‐processing approach and artefact handling, significantly influence mean flow index values, and previous studies correlating mean flow index with other established dCA metrics are confounded by inherent methodological flaws like heteroscedasticity, while the mean flow index also fails to discriminate individuals with presumed intact versus impaired dCA (discriminatory validity), and its prognostic performance (predictive validity) across various conditions remains inconsistent. The test–retest reliability, both within and between days, is generally poor. At present, no single approach for data collection or pre‐processing has proven superior for obtaining the mean flow index, and caution is advised in the further use of mean flow index‐based measures for assessing dCA, as current evidence does not support their clinical application.

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Emotion dysregulation in youths with obsessive-compulsive disorder and its implication for treatment - An exploratory study from the TECTO trial: A protocol and statistical analysis plan

Thoustrup,

Uhre,

Uhre

et al. 2025

Contemporary Clinical Trials Communications

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Physiolometrics and the puzzle of methodical acumen

Cited by 7 publications

References 19 publications

The reproducibility of dendritic cell and T cell counts to a 30‐min high‐intensity cycling protocol as a tool to highlight overtraining

The reproducibility of dendritic cell and T cell counts to a 30‐min high‐intensity cycling protocol as a tool to highlight overtraining

Myths and methodologies: Assessment of dynamic cerebral autoregulation by the mean flow index

Emotion dysregulation in youths with obsessive-compulsive disorder and its implication for treatment - An exploratory study from the TECTO trial: A protocol and statistical analysis plan

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