Phytase catalysis of dephosphorylation studied using isothermal titration calorimetry and electrospray ionization time-of-flight mass spectroscopy

Kempapidis, Theofilos; Bradshaw, N.; Hodges, Hayden E.; Cowieson, A. J.; Cameron, Duncan D.; Falconer, Robert J.

doi:10.1016/j.ab.2020.113859

Cited by 2 publications

(1 citation statement)

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“…Citrobacter braakii 6-phytase has five discrete cleavage steps and the reaction deviates radically from Michaelis-Menten kinetics. 54 The thermogram produced using a single injection of phytate into phytase had two peaks consistent with two periods of high enzyme activity with a bottleneck at the third cleavage step.…”

Section: Digestion Of Phytate (Myo-inositol Hexakisphosphate) Bymentioning

confidence: 93%

Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020

Falconer

Schuur

Mittermaier

2021

J of Molecular Recognition

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The last 5 years have seen a series of advances in the application of isothermal titration microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in understanding multiprotein complex formation including proteolysistargeting chimeras (PROTACS), and mitochondrial autophagy receptor Nix interaction with LC3 and GABARAP. It has also helped elucidate complex allosteric communication in protein complexes like trp RNA-binding attenuation protein (TRAP) complex.Advances in kinetics analysis have enabled the calculation of kinetic rate constants from pre-existing ITC data sets. Diverse strategies have also been developed to study enzyme kinetics and enzyme-inhibitor interactions. ITC has also been applied to study small molecule solvent and solute interactions involved in extraction, separation, and purification applications including liquid-liquid separation and extractive distillation. Diverse applications of ITC have been developed from the analysis of protein instability at different temperatures, determination of enzyme kinetics in suspensions of living cells to the adsorption of uremic toxins from aqueous streams.

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Section: Digestion Of Phytate (Myo-inositol Hexakisphosphate) Bymentioning

confidence: 93%