Phytase fromAspergillus niger

Volfová, O.; Dvořáková, Jana; Hanzlíková, A.; Jandera, A.

doi:10.1007/bf02814066

Cited by 43 publications

(21 citation statements)

References 10 publications

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“…Moreover in poultry the gut pH varies from 2.5 to 6.0, thus phytase active and stable in acidic environment is highly preferred (Radcliffe et al 1998). Among fungi many Aspergilli (Ullah 1988;Volfova et al 1994;Banerjee 2002, 2004;Vohra and Satyanarayana 2003;) are known to be active phytase producers. As Aspergillus niger is Generally Recognised as Safe (GRAS) it is frequently used in food and feed applications.…”

Section: Introductionmentioning

confidence: 99%

Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

Soni

Khire

2007

World J Microbiol Biotechnol

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Novel extracellular phytase was produced by Aspergillus niger NCIM 563 under submerged fermentation conditions at 30°C in medium containing dextrin and glucose as carbon sources along with sodium nitrate as nitrogen source. Maximum phytase activity (41.47 IU/mL at pH 2.5 and 10.71 IU/mL at pH 4.0) was obtained when dextrin was used as carbon source along with glucose and sodium nitrate as nitrogen source. Nearly 13 times increase in phytase activity was observed when phosphate in the form of KH 2 PO 4 (0.004 g/100 mL) was added in the fermentation medium. Physic-chemical properties of partially purified enzyme indicate the possibility of two distinct forms of phytases, Phy I and Phy II. Optimum pH and temperature for Phy I was 2.5 and 60°C while Phy II was 4.0 and 60°C, respectively. Phy I was stable in the pH range 1.5-3.5 while Phy II was stable in the wider pH range, 2.0-7.0. Molecular weight of Phy I and Phy II on Sephacryl S-200 was approximately 304 kDa and 183 kDa, respectively. Phy I activity was moderately stimulated in the presence of 1 mM Mg 2+ , Mn 2+ , Ca 2+ and Fe 3+ ions and inhibited by Zn 2+ and Cd 2+ ions while Phy II activity was moderately stimulated by Fe 3+ ions and was inhibited by Hg 2+ , Mn 2+ and Zn 2+ ions at 1 mM concentration in reaction mixture. The Km for Phy I and II was 3.18 and 0.514 mM while Vmax was 331.16 and 59.47 lmols/min/ mg protein, respectively.

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Section: Introductionmentioning

confidence: 99%

Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

Soni

Khire

2007

World J Microbiol Biotechnol

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“…The phytase activity in the ruminal strains was found to be cell-associated, even though substantial phytase activity was found in cell-free ruminal fluid, and phosphate was released from phytate into the culture fluid by pure cultures (Yanke et al 1998). Such cell-associated activity is in direct contrast to the extracellular phytase activity reported with Bacillus subtilis (Shimizu 1992) and Aspergillus niger (Volfová et al 1994). However, native phytase activity is expressed periplasmically in E. coli in the stationary phase, particularly under anaerobic conditions (Greiner et al 1993).…”

Section: [Traduit Par La Rédaction] Notes 395mentioning

confidence: 84%

Localization of phytase inSelenomonas ruminantiumandMitsuokella multiacidusby transmission electron microscopy

D'Silva¹,

Bae²,

Yanke³

et al. 2000

Can. J. Microbiol.

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The localization of phytase (myo-inositol-hexaphosphate phosphohydrolase) in the ruminal bacteria, Selenomonas ruminantium JY35 and Mitsuokella multiacidus 46/5(2), was determined with transmission electron microscopy. Phosphate produced from the enzymatic dephosphorylation of the calcium salt of phytic acid is precipitated as calcium phosphate. The calcium is then replaced with lead to produce electron-dense lead phosphate. This deposition of lead phosphate localized phytase in S. ruminantium JY35 and M. multiacidus 46/5(2) to the outer membrane, and confirmed intracellular expression of the enzyme in Escherichia coli pSrP.2, the recombinant clone which possesses the gene (phyA) encoding phytase (phyA) in S. ruminantium.

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“…(myo-inositol (1,2,3,4,5,6) hexakisphosphates) menjadi myo-inositol dan fosfat anorganik. Studi tentang fitase sangat pesat pada bebarapa tahun terakhir karena enzim ini bermanfaat terutama sebagai campuran pakan ternak guna mereduksi senyawa fitat, sehingga pemanfaatan unsur fosfor dalam tubuh ternak non ruminansia menjadi optimal (Greiner et al, 1997), serta guna mereduksi polusi unsur fosfor di lingkungan, sehingga eutrofikasi dipermukaan perairan dapat dicegah (Volfova et al, 1994, dan Shin et al, 2001.…”

Section: Abstrakunclassified

Isolasi dan Karakterisasi Fitase pada Mikrobia yang Terdapat pada Pupuk Kompos, Rumen Sapi, Ragi dan Tanah Sawah

Sajidan

2017

Sains Pet

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ABSTRAKPenelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi fitase dari berbagai sumber yang diasumsikan kaya akan senyawa fosfat komplek, seperti: pupuk kompos, isi rumen sapi dan ragi. Isolasi mikrobia dari pupuk kompos, isi rumen sapi dan ragi. Isolasi bakteri penghasil fitase dilakukan dengan menggunakan media LB (Luria Bertani) dengan inkubasi pada suhu 37 o C selama 16 jam, sedangkan isolasi mikrobia dari ragi menggunakan media PDA (Pottato Dextrose Agar). Ekstrak enzim kasar pada supernatan diperoleh dengan cara sentrifugasi pada kecepatan 5.000 rpm selama 5 menit. Ekstrak enzim kasar dikarakterisasi secara fisik meliputi pH dan temperatur optimum dan pengaruh efektor logam terhadap aktivitas enzim relatif. Hasil penelitian menunjukkan bahwa isolat B1 dari kompos P88 mepunyai aktivitas relatif optimum pada pH 5, suhu 50

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Phytase fromAspergillus niger

Cited by 43 publications

References 10 publications

Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions

Localization of phytase inSelenomonas ruminantiumandMitsuokella multiacidusby transmission electron microscopy

Isolasi dan Karakterisasi Fitase pada Mikrobia yang Terdapat pada Pupuk Kompos, Rumen Sapi, Ragi dan Tanah Sawah

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