Phytase produced on citric byproducts: purification and characterization

Abstract

Citric pulp is an agro-industrial residue from the citrus processing industry with low inorganic phosphorus content applied in animal feed. A new bioprocess was developed to produce and purify a new phytase generated on citric pulp fermentation by Aspergillus niger FS3. The phytase was purified by cationic-exchange, anionicexchange chromatography and chromatofocusing steps. From SDS-PAGE analysis, the molecular weight of the purified phytase was calculated to be 108 kDa. The phytase had an optimum pH of 5.0-5.5 and an optimum temperature of 60°C. The phytase displayed high affinity for phytate, and the K m was 0.52 mM. The purified phytase was sufficiently able to withstand pelleting temperatures, retaining sufficiently high phytate-degrading activity.

Formulated products containing a new phytase from Schyzophyllum sp. phytase for application in feed and food processing

“…Further studies would be needed to get purified phytase and to determine its molecular weight. It was in accordance with the phytases reported in the literature, which presented molecular mass between 60 and 110 KDa such as A. niger FS3 phytase with 108 KDa (Spier et al, 2010), A. niger FS3 SK-57 60 KDa (Nagashima etl al. ;1999), A. niger-307 39 KDa (Sariyska et al;; C, A. ficuum AS3.324 68.5 KDa (Zhang et al, 2005), Cladosporium sp.…”

“…This enzyme is utilized as feed animal supplement to breakdown the phytate, an antinutritional storage in wheat, barley, rice, corn and other cereal grains and seeds. It forms insoluble complexes with diand tri-valents ions such as zinc, magnesium, calcium and iron, decreasing their bioavailability (Spier et al, 2008(Spier et al, , 2010. In pigs, poultry, fishes and also human, phytase increases the phosphorus absorption, degrading the phytate of food in gastrointestinal transit (Sandberg, 2002;Pallauf and Rimbach, 1997).…”

“…Phytase must be thermotolerant to survive the high temperatures during animal feed pelleting and during the storage time. In addition, improvements in the enzyme stability may also increase its efficacy (Spier et al, 2010). Acid phosphatases (phytases) are used in the feed formulations to hydrolyze the phosphates to decrease the phosphorus pollution.…”

Bio-processing of agro-byproducts to animal feed

“…Citrus pulp is an agroindustrial residue from the citrus-processing industry with low inorganic phosphorus content applied in animal feed. A new bioprocess method was developed to produce and purify a new phytase generated on citruspulp fermentation by Aspergillus niger FS3 (Spier et al, 2010). Extracellular production of phytase by solid-state cultures of Malbranchea sulfurea and Aspergillus niveus was reported using cost-effective medium, such as wheat bran, corn bran, soybean meal, and cotton-seed meal.…”

Enhanced phytase production from Achromobacter sp. PB‐01 using wheat bran as substrate: Prospective application for animal feed

“…There are numerous reports on production of phytase from microorganisms 2, 9–17. However, reports on optimization of the production of high‐activity bacterial phytase by SmF of different agro‐industrial residues are very few.…”