Phytic acid modulates the morphology, immunological response of cytokines and β-defensins in porcine intestine exposed to deoxynivalenol and fumonisin B1

Silva, E.O. da; Santos, Jussevania Pereira; Morey, Alexandre Tadachi; Yamauchi, Lucy Megumi; Bracarense, Ana Paula Frederico Rodrigues Loureiro

doi:10.3920/wmj2020.2648

Cited by 3 publications

(2 citation statements)

References 60 publications

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“…Moreover, IL-10 is the most potent anti-inflammatory factor, which could decrease the inflammation-mediated organs injury [ 26 , 27 ]. Several evidence suggest that DON downregulated the mRNA levels of anti-inflammatory factor IL-10 in the fish and porcine intestine, which was in line with the results of the laying hens [ 28 , 29 ]. These findings suggested that DON-induced inflammatory injury of the intestine contributed to the release and recruitment of pro-inflammatory mediators and restrain anti-inflammatory factors, which promoted an inflammatory response and induced the intestinal barrier injury.…”

Section: Discussionsupporting

confidence: 81%

Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

Zhai

Qiu

Wang

et al. 2022

Toxins

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Deoxynivalenol is one the of most common mycotoxins in cereals and grains and causes a serious health threat to poultry and farm animals. Our previous study found that DON decreased the production performance of laying hens. It has been reported that DON could exert significant toxic effects on the intestinal barrier and microbiota. However, whether the decline of laying performance is related to intestinal barrier damage, and the underlying mechanisms of DON induced intestine function injury remain largely unclear in laying hens. In this study, 80 Hy-line brown laying hens at 26 weeks were randomly divided into 0, 1, 5 and 10 mg/kg.bw (body weight) DON daily for 6 weeks. The morphology of the duodenum, the expression of inflammation factors and tight junction proteins, and the diversity and abundance of microbiota were analyzed in different levels of DON treated to laying hens. The results demonstrated that the mucosal detachment and reduction of the villi number were presented in different DON treated groups with a dose-effect manner. Additionally, the genes expression of pro-inflammatory factors IL-1β, IL-8, TNF-α and anti-inflammatory factors IL-10 were increased or decreased at 5 and 10 mg/kg.bw DON groups, respectively. The levels of ZO-1 and claudin-1 expression were significantly decreased in 5 and 10 mg/kg.bw DON groups. Moreover, the alpha diversity including Chao, ACE and Shannon indices were all reduced in DON treated groups. At the phylum level, Firmicutes and Actinobacteria and Bacteroidetes, Proteobacteria, and Spirochaetes were decreased and increased in 10 mg/kg.bw DON group, respectively. At the genus levels, the relative abundance of Clostridium and Lactobacillus in 5 and 10 mg/kg.bw DON groups, and Alkanindiges and Spirochaeta in the 10 mg/kg.bw DON were significantly decreased and increased, respectively. Moreover, there were significant correlation between the expression of tight junction proteins and the relative abundance of Lactobacillus and Succinispira. These results indicated that DON exposure to the laying hens can induce the inflammation and disrupt intestinal tight junctions, suggesting that DON can directly damage barrier function, which may be closely related to the dysbiosis of intestinal microbiota.

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Section: Discussionsupporting

confidence: 81%

Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

Zhai

Qiu

Wang

et al. 2022

Toxins

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“…Current results show that PA cotreatment retains the axonal length and increases the survivability of CDDP-treated cells significantly ( P < 0.001). PA has been reported to have many beneficial effects in neuroprotection in Alzheimer’s disease, homeostasis, anticancer properties and inflammation control . Absorption of excessive iron, a significant factor in causing Alzheimer’s disease, was the reason behind neuroprotection .…”

Section: Discussionmentioning

confidence: 99%

Phytic Acid Maintains Peripheral Neuron Integrity and Enhances Survivability against Platinum-Induced Degeneration via Reducing Reactive Oxygen Species and Enhancing Mitochondrial Membrane Potential

Tiwari,

Albin,

Qubbaj

et al. 2024

ACS Chem. Neurosci.

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Phytic acid (PA) has been reported to possess anti-inflammatory and antioxidant properties that are critical for neuroprotection in neuronal disorders. This raises the question of whether PA can effectively protect sensory neurons against chemotherapy-induced peripheral neuropathy (CIPN). Peripheral neuropathy is a dose-limiting side effect of chemotherapy treatment often characterized by severe and abnormal pain in hands and feet resulting from peripheral nerve degeneration. Currently, there are no effective treatments available that can prevent or cure peripheral neuropathies other than symptomatic management. Herein, we aim to demonstrate the neuroprotective effects of PA against the neurodegeneration induced by the chemotherapeutics cisplatin (CDDP) and oxaliplatin. Further aims of this study are to provide the proposed mechanism of PA-mediated neuroprotection. The neuronal protection and survivability against CDDP were characterized by axon length measurements and cell body counting of the dorsal root ganglia (DRG) neurons. A cellular phenotype study was conducted microscopically. Intracellular reactive oxygen species (ROS) was estimated by fluorogenic probe dichlorofluorescein. Likewise, mitochondrial membrane potential (MMP) was assessed by fluorescent MitoTracker Orange CMTMRos. Similarly, the mitochondria-localized superoxide anion radical in response to CDDP with and without PA was evaluated. The culture of primary DRG neurons with CDDP reduced axon length and overall neuronal survival. However, cotreatment with PA demonstrated that axons were completely protected and showed increased stability up to the 45-day test duration, which is comparable to samples treated with PA alone and control. Notably, PA treatment scavenged the mitochondria-specific superoxide radicals and overall intracellular ROS that were largely induced by CDDP and simultaneously restored MMP. These results are credited to the underlying neuroprotection of PA in a platinum-treated condition. The results also exhibited that PA had a synergistic anticancer effect with CDDP in ovarian cancer in vitro models. For the first time, PA’s potency against CDDP-induced PN is demonstrated systematically. The overall findings of this study suggest the application of PA in CIPN prevention and therapeutic purposes.

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Foreword – special issue Mycotoxins in Latin America

Chulze,

Torres,

Torres

et al. 2021

WMJ

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Latin America with its considerable North-South extent is subject to climate that varies from tropical, subtropical and warm temperate to temperate. Different agricultural products are produced in the area including cereals, oilseeds, beans, fruits and nuts together with animal production including cattle for beef and milk, pigs, poultry and fish. The heterogeneity of agriculture in Latin America is reflected in the diversity of the region’s farm structures. While agriculture in the Southern Cone is dominated by large, commercial and export-oriented farms, particularly in Argentina and Brazil, besides increasingly in other countries like Uruguay, much of the rest of the region is characterised by smallholder and family agriculture. The contamination of agricultural products with mycotoxins has impact both human and animal health, as well as the economy due to losses related to rejections of agricultural products and by-products during trade. The economic burden related to the consumption of mycotoxins by animals is especially important, causing productivity losses up to the death of animals. The relevant mycotoxins are fumonisins, deoxynivalenol (DON) and zearalenone (ZEN) in cereals and cereal-based products, aflatoxins in cereals, oily seeds and nuts, aflatoxin M1 in milk and dairy products as well as ochratoxin A (OTA) in coffee, grapes and raisins. Co-occurrence of mycotoxins has also been observed mainly with aflatoxins and fumonisins in different Latin American countries (Torres et al., 2015). Advances on legislation in different countries including Argentina, Brazil, Chile, Mexico and Uruguay have been done to establish maximum limits for mycotoxins including aflatoxins, DON, ZEN, OTA, patulin and ergot alkaloids (ANVISA, 2011/2017; CAA, 2019/2021, Norma Oficial Mexicana, N.-243-S., 2010/2010; Reglamento Sanitario de los Alimentos, 2013).

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Phytic acid modulates the morphology, immunological response of cytokines and β-defensins in porcine intestine exposed to deoxynivalenol and fumonisin B1

Cited by 3 publications

References 60 publications

Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

Possible Toxic Mechanisms of Deoxynivalenol (DON) Exposure to Intestinal Barrier Damage and Dysbiosis of the Gut Microbiota in Laying Hens

Phytic Acid Maintains Peripheral Neuron Integrity and Enhances Survivability against Platinum-Induced Degeneration via Reducing Reactive Oxygen Species and Enhancing Mitochondrial Membrane Potential

Foreword – special issue Mycotoxins in Latin America

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