The production of silver nanoparticles (Ag NPs) utilizing biological means with renewable resources is thought to be risk-free, environmentally benign, and safe. In this work, the capacity of Bauhinia variegata to produce Ag NPs was measured. Numerous methods, including UV–Vis spectroscopy, TEM, FTIR spectroscopy, and XRD, were employed for the analysis of the produced Ag NPs. Ag NP antimicrobial capacity has been examined through microtitreplate as well as agar well diffusion techniques. Ag NPs’ ability to scavenge free radicals at varying concentrations was assessed using the DPPH technique. The MICs were 1,000 µg·mL−1 against pathogenic microbes including Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, while 500 and 250 µg·mL−1 were versus Bacillus subtilis and Escherichia coli, respectively. Silver showed an intriguing antioxidant capacity, achieving IC50 of 46.23 μg·mL−1. Additionally, Ag NPs demonstrated possible anticancer action when applied to the carcinoma cell lines Caco-2, with IC50 of 396.2 μg·mL−1 and cytotoxicity toward normal Vero cell lines with IC50 of 609.45 μg·mL−1. Furthermore, Ag NPs demonstrated a range of antibiofilm activities toward S. aureus (MRSA). In conclusion, Ag NPs biosynthesized via B. variegata show promise for a variety of safe biological applications.