Phytoactive Compounds from In Vitro Derived Tissues

Shahzad, Anwar; Shaheen, Arjumend; Kozgar, M. Imran; Sahai, Aastha; Sharma, Sreevalli

doi:10.1007/978-94-007-6603-7_1

“…According to study in tea cell suspension culture (Muthaiya et al, 2013), production of catechin is higher during the stationary phase rather than in both the lag phase and exponential phase. However based on a review (Shahzad et al, 2013), it has been shown that some plants such as Haplopappus, Rosa, Datura, Scopolia, Andrographis, Morinda and Poppulus were able to produce secondary metabolites in the early exponential phase. The secondary metabolites are anthocyanin formation by Haplopappus and Poppulus cells, phenolic Many bioactive compounds detected in this study are known to have a medicinal role.…”

mentioning

confidence: 99%

Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.)

Tunjung¹,

Fatonah

²

,

Christy³

et al. 2020

Indonesian J. Pharm.

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Our previous study showed that kaffir lime leaf extracts may have anticancer properties. However, production of bioactive compounds is affected by environmental factors. Here, we present a method to control environmental conditions using in vitro culture techniques such as callus induction. Calluses were induced from seed embryo explants of kaffir lime on MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1; and 2:1, respectively. Fourty and 60 days-old calluses were extracted using chloroform and ethyl acetate and analyzed by GC-MS. Results showed all combinations of 2,4-D and BAP were able to induce callogenesis from seed embryo explants of kaffir lime with no significant differences of callus initiation time, biomass, morphology and growth rates. However differences were detected in the bioactive compound profiles. In kaffir lime callus, both fatty acids and secondary metabolites were detected. Specifically, in 40 daysold calluses (exponential growth phase) we detected α-pinene and 1.8cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60 days-old calluses (stationary phase) we detected a number of compounds in plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including

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“…According to study in tea cell suspension culture (Muthaiya et al, 2013), production of catechin is higher during the stationary phase rather than in both the lag phase and exponential phase. However based on a review (Shahzad et al, 2013) Many bioactive compounds detected in this study are known to have a medicinal role. Our results warrant the further investigation of kaffir lime callus extracts for a more complete understanding of their potential for therapeutic use.…”

Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Novel Derivatives of 4-(6-(4-Substituted Phenyl)-7H-[1,2,4] Triazolo[3,4-b][1,3,4]Thiadiazin-3- yl) Phenol as a Potent Inhibitor of Tubulin with Antitumor Activity

Kubba¹,

Shihab²,

Al-Shawi³

et al. 2020

Indonesian J. Pharm.

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Our previous study showed that kaffir lime leaf extracts may have anticancer properties. However, production of bioactive compounds is affected by environmental factors. Here, we present a method to control environmental conditions using in vitro culture techniques such as callus induction. Calluses were induced from seed embryo explants of kaffir lime on MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1; and 2:1, respectively. Fourty and 60 days-old calluses were extracted using chloroform and ethyl acetate and analyzed by GC-MS. Results showed all combinations of 2,4-D and BAP were able to induce callogenesis from seed embryo explants of kaffir lime with no significant differences of callus initiation time, biomass, morphology and growth rates. However differences were detected in the bioactive compound profiles. In kaffir lime callus, both fatty acids and secondary metabolites were detected. Specifically, in 40 daysold calluses (exponential growth phase) we detected α-pinene and 1.8cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60 days-old calluses (stationary phase) we detected a number of compounds in plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including

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“…Atropa belladonna L. is an endangered perennial medicinal plant native to Europe and Asia, while Atropa acuminata Royle ex Miers, named Indian belladonna, is an Oriental native species also under threat of extinction. The chemical profile and morphology of A. acuminata are similar to that of A. belladonna, except for its flower color (Shahzad et al, 2013;Wani et al, 2007).…”

mentioning

confidence: 79%

“…acuminata are similar to that of A . belladonna , except for its flower color (Shahzad et al, 2013; Wani et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Methyl‐β‐cyclodextrin and coronatine as new elicitors of tropane alkaloid biosynthesis in Atropa acuminata and Atropa belladonna hairy root cultures

Fattahi

¹

,

Shojaeiyan

²

,

Palazón

³

et al. 2021

Physiologia Plantarum

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Hyoscyamine (HYO) and scopolamine (SCO) are tropane alkaloids acting as anticholinergic factors on the parasympathetic nervous system in humans and are produced by Solanaceous plants. Two strains of Agrobacterium rhizogenes, A4 and LBA9402, were used to infect Atropa acuminata Royle ex Miers and Atropa belladonna L. leaf explants. A. acuminata was inoculated either by direct infection or sonicated-assisted A. rhizogenes-mediated transformation (SAAT) was performed. A. belladonna was inoculated with the A4 strain using a direct method. The selected hairy root lines of both species were elicited with 50 mM methyl-β-cyclodextrin (β-CD), 0.5 μM coronatine (Cor) or 50 mM β-CD + 0.5 μM Cor on Day 14 of culture. The elicitor effect on growth and HYO and SCO content was analyzed after one (T1) and two (T2) weeks of treatment. In A. acuminata explants, the highest transformation percentage (T%) was obtained with strain A4 and the SAAT method (T%: 96.43). Cor significantly reduced the growth of A. acuminata hairy roots (fresh weight and dry weight [DW]: 2.52 and 0.3 g, respectively), whereas β-CD increased their DW (0.4 g). Also, the combined β-CD + Cor treatment had a positive significant effect on the DW of A. belladonna hairy roots (0.41 g). In A. acuminata hairy roots, the HYO level was lower under Cor treatment than in the control at both sampling times. In contrast, the SCO content was increased 10-fold by Cor elicitation at T1 compared to the control (10.95 mg g À1 DW) and was also positively affected by β-CD + Cor. In A. belladonna hairy roots, all the elicitors had a negative effect on both HYO and SCO production. This report is the first assessment of the effect of β-CD and Cor elicitors on tropane alkaloid production. 1 | INTRODUCTION Hyoscyamine (HYO) and scopolamine (SCO) are the most pharmacologically important tropane alkaloids, acting as anticholinergic factors on the parasympathetic nervous system in humans. These anticholinergic agents have extensive applications in medicine. They are used as anesthetics, antispasmodics, mydriatics, preoperative medication, sedatives, narcotics and in the treatment of asthma, cardiac and gastrointestinal disorders, Parkinson's disease and motion sickness

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Phytoactive Compounds from In Vitro Derived Tissues

Cited by 5 publications

References 134 publications

Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.)

Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.)

Design and Synthesis of Novel Derivatives of 4-(6-(4-Substituted Phenyl)-7H-[1,2,4] Triazolo[3,4-b][1,3,4]Thiadiazin-3- yl) Phenol as a Potent Inhibitor of Tubulin with Antitumor Activity

Methyl‐β‐cyclodextrin and coronatine as new elicitors of tropane alkaloid biosynthesis in Atropa acuminata and Atropa belladonna hairy root cultures

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