Phytochelatins are only partially correlated with Cd-stress in two species of Brassica

Gadapati, Winona; Macfie, Sheila M.

doi:10.1016/j.plantsci.2005.09.017

Cited by 45 publications

(23 citation statements)

References 36 publications

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“…The accumulation of Cd was higher in roots than in shoots. Many reports showed that most Cd taken up was retained in the roots (Gadapati and Macfie, 2006;Nouairi et al, 2006a, b;Singh et al, 2006;Wo´jcik et al, 2005). Our results agree with the above findings; we observed that around 75% of the Cd taken up was retained in roots (Table 4).…”

Section: Discussionsupporting

confidence: 92%

“…In comparison with other stressors, Cd-adapted plant cells exhibit a number of physiological changes, including considerable osmotic adjustments, which involve accumulation of organic solutes (Chen et al, 2001(Chen et al, , 2004Costa and Spitz, 1997;Ö ncel et al, 2000;Rai et al, 2005), vacuole compartmentalisation of ions (Seregin and Ivanov, 2001) and Cd sequestration by specific molecules such as phytochelatins (PCs) and/or metallothioneins (Gadapati and Macfie, 2006;Mishra et al, 2006;Murphy et al, 1997;Stolt et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake

Nedjimi

Daoud

2009

Flora - Morphology, Distribution, Functional Ecology of Plants

193

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake

Nedjimi

Daoud

2009

Flora - Morphology, Distribution, Functional Ecology of Plants

193

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“…Since NA is known to play a key role in ion homeostasis and long-distance transport, we investigated whether the ion content (copper [Cu], iron [Fe], zinc [Zn], and manganese [Mn]) of mtn1-1mtn2-1 was different from that in the wild type, using inductively coupled plasma atomic emission spectroscopy (Gadapati and Macfie, 2006). In the mutant inflorescences, contents of Cu, Fe, and Zn were significantly lower, with Fe and Zn showing the greatest reduction compared with the wild type (P , 0.05; Fig.…”

Section: Mtn Deficiency Results In Altered Na Levelsmentioning

confidence: 99%

“…1573a) leaves were also processed without sample to quantify the recovery of Zn, Cu, Mn, and Fe during the extraction procedure. Filtered samples were prepared and analyzed for metals as described by Gadapati and Macfie (2006) by inductively coupled plasma atomic emission spectroscopy.…”

Section: Detection Of Metal Contentsmentioning

confidence: 99%

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

et al. 2012

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5#-Methylthioadenosine (MTA) is the common by-product of polyamine (PA), nicotianamine (NA), and ethylene biosynthesis in Arabidopsis (Arabidopsis thaliana). The methylthiol moiety of MTA is salvaged by 5#-methylthioadenosine nucleosidase (MTN) in a reaction producing methylthioribose (MTR) and adenine. The MTN double mutant, mtn1-1mtn2-1, retains approximately 14% of the MTN enzyme activity present in the wild type and displays a pleiotropic phenotype that includes altered vasculature and impaired fertility. These abnormal traits were associated with increased MTA levels, altered PA profiles, and reduced NA content. Exogenous feeding of PAs partially recovered fertility, whereas NA supplementation improved fertility and also reversed interveinal chlorosis. The analysis of PA synthase crystal structures containing bound MTA suggests that the corresponding enzyme activities are sensitive to available MTA. Mutant plants that expressed either MTN or human methylthioadenosine phosphorylase (which metabolizes MTA without producing MTR) appeared wild type, proving that the abnormal traits of the mutant are due to MTA accumulation rather than reduced MTR. Based on our results, we propose that the key targets affected by increased MTA content are thermospermine synthase activity and spermidinedependent posttranslational modification of eukaryotic initiation factor 5A.

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“…The response of plants to heavy metal ions (and also cadmium) exposure on molecular level is connected with complex process mainly based on the modulation of transcriptional activity of stress-related genes (Mazzucotelli et al 2008). Exposure of plants to heavy metals results in activation of protective system, which enhances the synthesis of the protective low molecular mass thiol compounds (Grill et al 1985;Tukendorf and Rauser 1990;Rauser 1995Rauser , 1999VogeliLange and Wagner 1996;Cobbett and Goldsbrough 2002;Zhang et al 2005;Gadapati and Macfie 2006;Jamali et al 2007). The ability of a plant to withstand heavy metal's effects can be used in a technology called phytoremediation, treatment of environmental problems using plants (Salt et al 1998;Diopan et al 2007;Stejskal et al 2008).…”

Section: Introductionmentioning

confidence: 99%

The role of sulphur in cadmium(II) ions detoxification demonstrated in in vitro model: Dionaea muscipula Ell.

et al. 2008

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Fertilization by phosphorus and also nitrogen fertilizers are the main source of cadmium soil contamination. Cadmium is relatively easily taken up by plants and then consequently enters their food chain. We investigated whether the cultivation medium with or without sulphates supplement affects the synthesis of protective low molecular mass thiols such as cysteine, reduced glutathione, oxidized glutathione and phytochelatins (PC2) in Dionaea muscipula treated with cadmium(II) ions at 0, 1, 5, 10, 25, 50, 100, 250, 500 and 1,000 lM for 6 weeks. The plants cultivated in the presence of sulphates showed higher cadmium tolerance due to faster growth and lesser number of necrosis hallmarks.Further, we utilized liquid chromatography with electrochemical detection for determination of the thiols in plant tissues. In the case of the plants cultivated on media supplemented with sulphates for 6 weeks the average PC2 level was 2,830 ng/g of fresh weight (FW). However, the plants cultivated on media without the presence of sulphates on average contained 1,160 ng PC2/g FW. Results obtained showed the positive effect of sulphur supplementation in cadmium detoxification processes in plants. In addition to thiol content, we also determined level of majority secondary metabolite of Venus flytrap, naphthoquinone plumbagin. Generally, the presence of sulphates in the media enhanced the protective mechanism and did not affect directly the synthesis of secondary metabolite plumbagin.

show abstract

Phytochelatins are only partially correlated with Cd-stress in two species of Brassica

Cited by 45 publications

References 36 publications

Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake

Cadmium accumulation in Atriplex halimus subsp. schweinfurthii and its influence on growth, proline, root hydraulic conductivity and nutrient uptake

Recycling of Methylthioadenosine Is Essential for Normal Vascular Development and Reproduction in Arabidopsis

The role of sulphur in cadmium(II) ions detoxification demonstrated in in vitro model: Dionaea muscipula Ell.

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