Phytochemical Analysis and Biological Activity of Salvia compressa Vent.

Noorbakhsh, Firoozeh; Zare, Somayeh; Firuzi, Omidreza; Sakhteman, Amirhossein; Chandran, Jima N.; Schneider, Bernd; Jassbi, Amir Reza

doi:10.5812/ijpr-127031

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Based on 1 H-and 13 C NMR spectral data of compound 8, it was characterized as a fatty acid (Noorbakhsh et al 2022). As seen in Supplementary Information Table 6., the ole nic protons signals was detected at δ 5.35 ppm while the H-2 and H-3 and the allylic signals were recorded at δ 2.34 (t J= 7.5), 2.02 m, and 1.61 ppm, respectively.…”

Section: Structure Elucidation Of Fatty Acid Compoundsmentioning

confidence: 99%

Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

Moheimanian,

Mirkhani,

Edraki

et al. 2023

J Appl Phycol

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Anti-diabetic potential of a brown alga, Padina tetrastromatica, from the coastline of the Persian Gulf, using bioassay-guided puri cation approach yielded the most active α-glucosidase inhibitor agents as fatty acids. Initially, the yeast's α-glucosidase inhibition of the MeOH and 80% MeOH extracts were evaluated via a colorimetric assay. The liquid-liquid fractionation of 80% MeOH extract, as the most potent α-glucosidase inhibitor, resulted in four fractions, n-hexane, ethyl acetate, n-butanol and water. Hexane and ethyl acetate fractions were selected for further studies, with the IC 50 values of 38.0 ± 0.3 µg/mL and 53.7 ± 2.6 µg/mL, respectively. Followed by investigating α-glucosidase inhibition of the sub-fractions from the hexane fraction, using ash column chromatography, F18-21 selected as the most potent enzyme inhibitor. After further puri cations of F18-21 by semi preparative HPLC, the mentioned fraction and two puri ed compounds, 8-octadecenoic acid (8) and allcis-5,8,11,14-eicosatetraenoic acid (10) were subjected to GC-MS analysis for the identi cation. In addition, 1D and 2D NMR evaluations were performed for characterisation of 8-octadecenoic acid. Furthermore, three fatty acids, all-cis-8,11,14,17eicosatetraenoic acid (6), cis-9,12-octadecadienoic acid ( 7), and all-cis-5,8,11,14,17-eicosapentaenoic acid (11), were isolated from the ethyl acetate fraction and identi ed, by HPLC and GC-MS techniques, respectively. Finally, α-glucosidase inhibition percent of the puri ed fatty acids were evaluated in two concentrations in the microplates, which showed their great potential for further investigations as anti-diabetic agents, in comparison with acarbose, as the positive control. Furthermore, molecular docking analysis and MD simulation were applied to investigate the structure activity of the puri ed compounds.

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Section: Structure Elucidation Of Fatty Acid Compoundsmentioning

confidence: 99%

Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

Moheimanian,

Mirkhani,

Edraki

et al. 2023

J Appl Phycol

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“…Medicinal plants are a major depository of secondary metabolites that might be a source of new organic pesticides. Natural pesticides are less harmful to human health, are more environment friendly, less expensive, and are easier to use in integrated pest control compared to synthetic ones [5]. The development of herbalbased pesticides such as essential oils, extracts, and pure chemicals, and their formulations, is underway to offset the negative effects of synthetic pesticides [6].…”

Section: Introductionmentioning

confidence: 99%

Chemo-profiling of methanolic and ether oleoresins of Salvia coccinea and in vitro pesticidal evaluation with in silico molecular docking and ADME/Tox studies

et al. 2023

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The objective of the present study was to examine the chemical compositions of Salvia coccinea oleoresins prepared in methanol and petroleum ether. GC-MS analysis of Salvia coccinea methanolic oleoresin (SCMO) and Salvia coccinea ether oleoresin (SCEO) resulted in the identification of 15 and 12 constituents, comprising 84.7 and 81.2% of the total composition, respectively. Both SCMO and SCEO varied in their chemical composition in terms of quantity, namely, oleic acid (22.3-25.9%), palmitic acid (8.9-8.4%), stigmasta-3,5-dien-7-one (3.4-11.8%), stigmasterol acetate (3.5-5.3%), neophytadiene (4.8-1.7%), phytol (1.6-7.8%) and phthalic acid (2.1-3.1%). In addition to the qualitative differences between SCMO and SCEO concomitantly, both oleoresins were examined for their pesticidal activities. Oleoresins demonstrated significant nematicidal activity against Meloidogyne incognita, insecticidal activity against Lipaphis erysimi, antifungal activity against Curvularia lunata, and antibacterial activity against Staphylococcus aureus. For nematicidal activity, SCMO and SCEO exhibited a high mortality of 65.66±1.69 and 54.33±1.24 and egg hatching inhibition of 26.33±1.20and 33.33±1.24 at 200 μg/mL. Similarly, SCMO and SCEO exhibited excellent insecticidal activity with 94.87±1.44 % and 86.75±1.85 % mortality at 1000 μg/mL. However, both oleoresins exhibited moderate antifungal and antibacterial activities compared to standards. Due to the quantitative difference in chemical composition and the presence of several phytoconstituents that were absent in SCEO, SCMO displayed stronger pesticidal effects than SCEO. To estimate the binding energy and structure-activity relationships between chemical constituents and pesticidal activities, in silico molecular docking and ADME/Tox studies have also been performed using a web-based online tool. On the basis of the present study, it is inferred that the herb Salvia coccinea might be a good source of phytochemicals and can be used for the development of herbal-based pesticides/formulations after proper clinical trials.

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Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

Moheimanian

Mirkhani

Edraki

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Anti-diabetic potential of a brown alga, Padina tetrastromatica, from the coastline of the Persian Gulf, using bioassay-guided purification approach yielded the most active α-glucosidase inhibitor agents as fatty acids. Initially, the yeast’s α-glucosidase inhibition of the MeOH and 80% MeOH extracts were evaluated via a colorimetric assay. The liquid-liquid fractionation of 80% MeOH extract, as the most potent α-glucosidase inhibitor, resulted in four fractions, n-hexane, ethyl acetate, n-butanol and water. Hexane and ethyl acetate fractions were selected for further studies, with the IC50 values of 38.0 ± 0.3 µg/mL and 53.7 ± 2.6 µg/mL, respectively. Followed by investigating α-glucosidase inhibition of the sub-fractions from the hexane fraction, using flash column chromatography, F18-21 selected as the most potent enzyme inhibitor. After further purifications of F18-21 by semi preparative HPLC, the mentioned fraction and two purified compounds, 8-octadecenoic acid (8) and all-cis-5,8,11,14-eicosatetraenoic acid (10) were subjected to GC-MS analysis for the identification. In addition, 1D and 2D NMR evaluations were performed for characterisation of 8-octadecenoic acid. Furthermore, three fatty acids, all-cis-8,11,14,17-eicosatetraenoic acid (6), cis-9,12-octadecadienoic acid (7), and all-cis-5,8,11,14,17-eicosapentaenoic acid (11), were isolated from the ethyl acetate fraction and identified, by HPLC and GC-MS techniques, respectively. Finally, α-glucosidase inhibition percent of the purified fatty acids were evaluated in two concentrations in the microplates, which showed their great potential for further investigations as anti-diabetic agents, in comparison with acarbose, as the positive control. Furthermore, molecular docking analysis and MD simulation were applied to investigate the structure activity of the purified compounds.

show abstract

Phytochemical Analysis and Biological Activity of Salvia compressa Vent.

Cited by 3 publications

References 23 publications

Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

Chemo-profiling of methanolic and ether oleoresins of Salvia coccinea and in vitro pesticidal evaluation with in silico molecular docking and ADME/Tox studies

Bioassay-guided purification of α-glucosidase inhibitor fatty acids from Padina tetrastromatica

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