Phytochemical Analysis and Escherichia coli Anti-bacteria Activity of Teak (Tectona grandis Linn. f) Leaves Extract

Abstract

Teak (Tectona grandis L.f.) is a medicinal plant that has been traditionally used by people around the forest as medicine. Teak leaves contain antibacterial compounds. This study aims to find out what compounds are contained in teak leaves extract and test antibacterial activity against E. coli bacteria. The methods used in this study are maceration for leaves extract, qualitative methods for phytochemical tests, and descriptive research methods with laboratory analysis techniques. The phytochemical analysis will be tested for alkaloids, flavonoids, saponins, tannins, triterpenoids, steroids, and carotenoids. And antibacterial tests against E. coli bacteria consisting of 7 treatments, with five constituents, namely 20%, 40%, 60%, 80%, and 100%. One positive control using chloramphenicol 0.1% and one negative control using aquades. The data obtained will be presented descriptively by looking at the color change and the shape of the liquid being tested, the data obtained will be presented in the form of pictures and tables. The results showed that teak leaves extract positively contained flavonoids flavonoid compounds, tannins, and steroids. While alkaloid compounds, saponins, triterpenoids, and carotenoids showed negative results. For the results of antibacterial tests of Teak leaf extract, using 96% ethanol solvent against Escherichia coli bacteria with an average value of inhibitory power at a concentration of 20% (13.73 mm), a concentration of 40% (16.36 mm), a concentration of 60% (17.43 mm), a concentration of 80% (18.66), and a concentration of 100% (19.42 mm), the higher the concentration, the greater the inhibition zone formed.