Phytochemical Analysis and Evaluation of the Antioxidant, Antiproliferative, Antibacterial, and Antibiofilm Effects of Globularia alypum (L.) Leaves

Abstract

Globularia alypum L. (GA) is a Mediterranean plant of the Globulariaceae family which is widely used in traditional Tunisian medicine. The main goal of this study was to evaluate the phytochemical composition, antioxidant, antibacterial, and antibiofilm activities, and the antiproliferative potential of different extracts of this plant. The identification and the quantification of the different constituents of extracts were determined using gas chromatography–mass spectrometry (GC-MS). The antioxidant activities were evaluated using spectrophotometric methods and chemical tests. The antiproliferative study was based on the use of colorectal cancer SW620 cells, including an antibacterial assessment with the microdilution method and analysis of the antibiofilm effects via the crystal violet assay. All extracts presented several components, mainly sesquiterpenes, hydrocarbon, and oxygenated monoterpenes. The results revealed that the maceration extract had the most important antioxidant effect (IC50 = 0.04 and 0.15 mg/mL), followed by the sonication extract (IC50 = 0.18 and 0.28 mg/mL). However, the sonication extract demonstrated significant antiproliferative (IC50 = 20 µg/mL), antibacterial (MIC = 6.25 mg/mLand MBC > 25 mg/mL), and antibiofilm (35.78% at 25 mg/mL) properties against S. aureus. The results achieved confirm the important role of this plant as a source of therapeutic activities.

HPLC-DAD Analysis and Investigation of Biological Properties of the Leaves of Globularia alypum (L.), Infusion Extract

“…For instance, we previously reported that sonication-obtained extracts of G. alypum demonstrated a noteworthy antibacterial effect on S. aureus, exhibiting a zone of inhibition of 14.5 mm that aligns with our actual study. However, these methanolic extracts demonstrated no effect against the remaining strains tested [31]. In addition to other water-soluble components that were naturally present in the plant material, Nayak et al speculated that the anionic components, such as chlorides, thiocyanate, sulfates, and nitrate, may be responsible for the antibacterial action of aqueous extracts [32].…”

Analysis of Pathogens of Urinary Tract Infections Associated with Indwelling Double-J Stents and Their Susceptibility to Globularia alypum

“…All segments of the stent parts, including the inner surface, middle, and outer surface, were cultured according to the technique described by Brun-Buisson et al [18], adapted from Cleri et al [19]. In brief, 1 mL of sterile water (physiological serum) was added to the tube containing the corresponding segment.…”