Phytochemical Analysis and Evaluation of in Vitro Immunomodulatory Activity of Rhododendron Arboreum Leaves

Rawat, Pramod; Bachheti, Rakesh Kumar; Kumar, Navin

doi:10.22159/ajpcr.2018.v11i8.25372

Cited by 19 publications

(10 citation statements)

References 13 publications

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“…4). The literature survey result of the reported compounds from this research showed to have potent herbal drug resources [15]. This emphasizes the plant's importance in homeopathic alignment as a healthcare product.…”

Section: Results Of Peak Chromatogramsupporting

confidence: 51%

Qualitative Analysis of Rhododendron arboreum Leaves Extracts using HPLC-ESI-QTOF-MS

Sharma¹,

Lamichhane²

2019

Biotechnol Ind J

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Nepal has the richest source of herbal medicinal plants and high plant biodiversity even within the altitudinal variation of every100 meter. Based on the high biodiversity report cell-mediated the national flower of Nepal, Rhododendron arboreum (Smith), was collected from Palpa district of Nepal with an altitude of 1350 and phytochemical analysis was performed from its methanol extract. Basic Phytochemical analysis showed the presence of Alkaloids, Flavonoids Coumarins, and Saponins. Based on primary research output and the importance of the basic compounds the samples were further reanalyzed with HPLC-ESI and QTOF-MS. Thirteen different chemically important compounds from R. arboreum were identified by HPLC-ESI-QTOF-MS. Among them, Rhodolatouside A and Rhodolatouside B were first time reported as new compounds in Rhododendron arboreum from Nepal whereas Coumaric acid, Gallic acid, Rhodolatouside A, Leuco-pelargonidin, Rhodolatouside B, Myricetin, Hyperoside, Quercetin, Maslinic Acid, Linoleic acid, 1-Naphthalenepropanol, Octadecanoic acid, and Betulinic Acid/Ursolic acid were observed as previously reported compounds. All the compounds were identified with their molecular formula.

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Section: Results Of Peak Chromatogramsupporting

confidence: 51%

Qualitative Analysis of Rhododendron arboreum Leaves Extracts using HPLC-ESI-QTOF-MS

Sharma¹,

Lamichhane²

2019

Biotechnol Ind J

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“…All the extracts were undergone preliminary phytochemical analysis to identify the nature of secondary metabolites such as alkaloids, carbohydrate, glycosides, saponins, steroids, terpenoids, phenolics, flavonoids and protein present in the plant [16].…”

Section: Preliminary Phytochemical Screeningmentioning

confidence: 99%

Pharmacognostical and Phytochemical Standardization of Physalis Minima L. Leaf

Shil¹,

Laloo²,

Das³

et al. 2019

Int J Pharm Pharm Sci

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Objective: To evaluate the pharmacognostical and phytochemical parameters of Physalis minima leaf. Methods: The leaf of Physalis minima was examined for macroscopical, microscopical, physicochemical parameters and fluorescence analysis. Extracts obtained from the leaf was analyzed for phytochemical screening and estimation of total tannin, phenolic and flavonoid content following the standard procedure available in the literature. Results: Morphologically, the leaf was found to be ovate in shape, 5 to 8 cm long and up to 3 cm width with dentate margin, asymmetrical base, hairy surface, reticulate veins on each side of midrib, green in colour, characteristic odour and slight bitter in taste. Microscopic study has shown the important diagnostic characters of Solanaceae family which is characterized by presence of dorsiventral leaf with anomocytic stomata, grandular or uniseriate trichomes and cluster crystal of calcium oxalate. Physicochemical parameters like foreign organic matter (0.78%), loss on drying (8.23%), total ash (11.4%), acid insoluble ash (2.2%), water-soluble ash (6.1%), sulphated ash (2.4%) alcohol soluble extractive (10.4%), water-soluble extractive (9.5%), ether soluble extractive (1.8%) foaming index (below 100), swelling index (1), volatile oil and heavy metal content were quantified. Phytochemical analysis of different extracts of Physalis minima leaf has shown the presence of phytoconstituents viz. alkaloids, steroids, tannin, flavonoids, protein. Quantification of phytoconstituents was also reported like phenols (10.59±0.65 mg/gm equivalent to tannic acid), tannin (8.24±0.27 mg/gm equivalent to tannic acid) and flavonoids (87.17±0.87 mg/gm equivalent to rutin) respectively. Conclusion: This present study was provided the qualitative and quantitative standard of Physalis minima will help to prevent the possible steps of adulteration with other species of the same genus.

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“…The different solvent extracts of the bark of Cascabela thevetia L. obtained were separately tested for the presence of various plant constituents such as alkaloids, flavonoids, glycosides, steroids, tannins, phenolic compounds, carbohydrates, saponins, triterpenoids, anthraquinones and fatty acids as per the standard procedure available [11,12].…”

Section: Preliminary Phytochemical Analysismentioning

confidence: 99%

Pharmacognostic Standardization, Preliminary Phytochemical Screening and TLC Fingerprinting of the Bark of Cascabela Thevetia L.

Gogoi

Bhuyan

Deka

2019

Int J Pharm Pharm Sci

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Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

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Phytochemical Analysis and Evaluation of in Vitro Immunomodulatory Activity of Rhododendron Arboreum Leaves

Cited by 19 publications

References 13 publications

Qualitative Analysis of Rhododendron arboreum Leaves Extracts using HPLC-ESI-QTOF-MS

Qualitative Analysis of Rhododendron arboreum Leaves Extracts using HPLC-ESI-QTOF-MS

Pharmacognostical and Phytochemical Standardization of Physalis Minima L. Leaf

Pharmacognostic Standardization, Preliminary Phytochemical Screening and TLC Fingerprinting of the Bark of Cascabela Thevetia L.

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