Phytochemical Analysis and hypoglycemic potential of Filago hurdwarica (Wall. ex DC.) Wagenitz in alloxan induced diabetic mice

Abstract

Plants have profound therapeutic benefits, more economical treatments, fewer side effects, and a relatively cheap cost, making them a source of drugs for protective, preventative, curative, or conducive purposes and creating novel phytomedicines. Plant derived medicines are relatively safe compared to synthetic medicines. Many plants have proved to successfully aid in the treatment of diabetes including Filago hurdwarica (Wall. ex DC.) Wagenitz. The current investigations were therefore designed to assess the phytochemical, antioxidant, antidiabetic, and antihyperlipidemic activities of F. hurdwarica. The phytochemical investigations and antioxidant activities of different extracts were carried out using standard chemical tests, DPPH, and H2O2 scavenging assays. F. hurdwarica plant extract in Hydromethanolic solution were prepared by Soxhletation method and stored in refrigerator at 4°C for two days before use. Swiss Albino mice were made diabetic by a single dose of alloxan (150 mg/kg). Hydromethanolic plant extract and fractions of F. hurdwarica were screened for antidiabetic activity and given to the alloxan-induced diabetic mice at a concentration of 150-250 mg/kg of body weight in different groups of 6 diabetic mice each orally once a day for 15 days. Glibenclamide is also given to another group to as a standard drug to support the result at a dose of 10 mg/kg of body weight orally once a day for 15 days. Blood glucose levels and body weights of mice were measured on 0, 4, 7, 11 and 15th days. The study found that the extract was safe up to the dose level of 2000 mg/kg and the dose response effect of chloroform extract (150-250 mg/kg) of F. hurdwarica showed expressive antihyperglycemic effects and also improved other altered biochemical parameters associated with diabetes. The FTIR and XRD spectra demonstrated the occurrence of phenols, alcohols, alkenes, alkyl halides, ketones, and aromatic compounds and confirmed the amorphous nature of the extract. GC-MS spectral analysis showed the tentative presence of 31 phytochemical constituents in the chloroform extract of F. hurdwarica with different retention time. To conclude, the chloroform extract (250 mg/kg) of F. hurdwarica revealed considerable antioxidant, antihyperglycemic, and antihyperlipidemic potential and is safe for treating diabetes and related complications.

Phytochemical Investigation and Therapeutical Potential of Cotinus coggygria Scop. in Alloxan-Induced Diabetic Mice

“…Due to their capacity to stimulate glycogenesis in the liver, release insulin from pancreatic β -cells, or inhibiting glucose absorption in the gut, the secondary metabolites that were found in C. coggygria extracts may be responsible for the antioxidant and observed glucose suppressive and antihyperglycemic activities [ 79 – 82 ]. Through a number of mechanisms, the utilization of these plants and phytoconstituents may prevent the onset of diabetic complications and regulate metabolic irregularities, which is in accordance with our previous research work [ 83 , 84 ]. In order to integrate plant medicines with high therapeutic effects to treat diabetes, these results stimulate future investigations of the extracts and identify particular active molecules engaged in various biological functions.…”

Reducing Sugar, Alkaloid and Tannin from Dryopteris dilatata Fractions Modulates Diabetogenic and Oxidative Stress Activity on Alloxan Induced Diabetic Rats

“…In this study pre-treatment with plant fractions before glucose administration on wistar reveals the glucose tolerance capacity of the Observed reduction of body weight in alloxaninduced diabetic rats in present study is known to be associated with diabetes mellitus due to destruction of pancreatic beta cell and subsequent reduction of insulin. It may be as a result of muscle wasting, breakdown of complex molecules like carbohydrates, fats and proteins [24]. Treatment with fractions of Dd showed statistically significant increase in body weight compared to diabetic control revealing the therapeutic activity of Dd in muscle wasting and breakdown of complex molecules, modulating the activity of oxidative stress and gluconeogenesis observed in diabetic state [25].…”

Impact of Nutraceuticals on Type 1 and Type 2 Diabetes Mellitus-Induced Micro- and Macrovasculopathies

“…Because medicinal plants offer anti-diabetic and hypoglycemic qualities, they are used as medication and targeted by pharmaceutical companies for their properties [84]. They are also affordable to the general public [85].…”