Phytochemical analysis by HPLC-PDA/ESI-MS of Globularia alypum aqueous extract and mechanism of its protective effect on experimental colitis induced by acetic acid in rat

Hajji, Najla; Jabri, Mohamed-Amine; Tounsi, H.; Wanes, Dalanda; Ali, Imen Ben; Boulila, Abdennacer; Marzouki, Lamjed; Sebaï, Hichem

doi:10.1016/j.jff.2018.05.058

Cited by 17 publications

(22 citation statements)

References 56 publications

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“…In a previous study, we determined the chemical composition of GAAE which was provided by flavonoids, iridoids, and phenylethanoid glucosides compounds. 10 Those secondary metabolites are known to exert several biological activities. [27][28][29] In this case, we investigated the purgative/laxative effects of GAAE on gastric emptying and GIT, and on constipation induced by LOP drug, testing the same doses (100, 200 and 400 mg/mL) used previously either in gastrointestinal motility or in colon inflammation which gave significant therapeutic properties with a minimum or any toxic side effects.…”

Section: Discussionmentioning

confidence: 99%

“…[27][28][29] In this case, we investigated the purgative/laxative effects of GAAE on gastric emptying and GIT, and on constipation induced by LOP drug, testing the same doses (100, 200 and 400 mg/mL) used previously either in gastrointestinal motility or in colon inflammation which gave significant therapeutic properties with a minimum or any toxic side effects. 10,30 However, different equations should be used for dose extrapolation among Human or other species. 31 Therefore, the limits of the universal adequacy of herbal medicines due to the lack of standardized chemical characterization, the dosing Rats were treated with LOP and GAAE or yohimbine over seven days.…”

Section: Discussionmentioning

confidence: 99%

“…Data from a previous study have already showed the capacity of GAAE in protecting against colonic inflammation in rats. 10 The oxidative status was evaluated in treated colons by measurement of some oxidant and antioxidant reactors. In this respect, it is showed that constipation induced by LOP in animals increase in turn the oxidative stress in the body that is exhibited with augmented levels of MDA, H 2 O 2 , and OHas well as depleted levels of enzymatic and non-enzymatic antioxidants such as SOD, GPx, and -SH groups.…”

Section: Discussionmentioning

confidence: 99%

“…The analyses of the aqueous extract of GA leaves showed its abundance in secondary metabolites including flavonoids, iridoids, and phenylethanoid glucosoides. 10 GA extract showed many in vivo and in vitro biological activities such as anti-inflammatory activity against acetic acid-induced colitis in rats, 10 antioxidant effect against carrageenan-induced paw-edema in mice, 11 in vitro antimicrobial activity, 12 spermatogenesis activation in rats, 13 and vasodilatory effect in rat mesenteric arterial bed. 14 In the present study, we investigate the purgative/laxative effects of an aqueous extract of GA (GAAE) on gastrointestinal-physiological functions and against loperamide (LOP)-induced constipation coupled to oxidative stress in rats.…”

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Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal‐physiological function and against loperamide‐induced constipation coupled to oxidative stress and inflammation in rats

Hajji

Wannes

Jabri

et al. 2020

Neurogastroenterology Motil

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Background Chronic constipation is a gastrointestinal functional disorder which affects patient quality of life. Therefore, many studies were oriented to search herbal laxative agents. In this study, we investigated the effect of Globularia alypum L. leaves aqueous extract (GAAE) against loperamide (LOP)‐produced constipation. Methods Animals were given LOP (3 mg/kg, b.w., i.p.) and GAAE (100, 200, and 400 mg/kg, b.w., p.o.) or yohimbine (2 mg/kg, b.w., i.p.), simultaneously, for 1 week. Gastric‐emptying test and intestinal transit were determined. Colon histology was examined, and oxidative status was evaluated using biochemical‐colorimetric methods. Key Results GAAE ameliorates significantly gastric emptying (64% to 76.5%) and intestinal transit (66.65% to 84.73%). LOP negatively influenced defecation parameters and generated a stress situation. GAAE administration in contrast ameliorated those parameters and re‐established oxidative balance. Conclusion GAAE showed a modest action against oxidative stress and decreased LOP effect and thereby can be considered a pharmacological agent in constipation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal‐physiological function and against loperamide‐induced constipation coupled to oxidative stress and inflammation in rats

Hajji

Wannes

Jabri

et al. 2020

Neurogastroenterology Motil

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“…High-resolution mass spectral data was obtained on a Thermo Instruments ESI-MS system (LTQ XL/LTQ Orbitrap Discovery, UK) connected to a Thermo Instruments HPLC system (Accela PDA Detector, Accela PDA Autosampler and Accela Pump). 15…”

Section: Tv-ae Bioactive Compound Identication By Liquid Chromatogramentioning

confidence: 99%

The potential of Thymus vulgaris aqueous extract to protect against delayed gastric emptying and colonic constipation in rats

et al. 2019

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Banxia Xiexin decoction ameliorates dextran sulfate sodium (DSS)‐induced ulcerative colitis via inhibiting serine‐threonine protein kinase (Akt)/mitogen‐activated protein kinase (MAPK) signaling pathway

Liu

Huo

et al. 2023

Biotech and App Biochem

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Banxia Xiexin decoction (BXD), a traditional Chinese medicine, was widely used in treating ulcerative colitis (UC). However, the active components of BXD and its mechanism in UC remain elusive. Therefore, we used network pharmacology in vivo experiments, molecular docking, and surface plasmon resonance strategy (SPR) to uncover BXD's potential mechanism. A UC rat model was established by orally administering 7% dextran sulfate sodium (DSS) in drinking water, BXD and palmatine were orally administered for 7 days. Network pharmacology was used to investigate the main bioactive components and crucial targets of BXD in treating UC. Molecular docking was used to investigate interactions between components and crucial targets, verifying the results by SPR. By network pharmacology predicting, 20 active components and 44 candidate anti‐UC targets of BXD were identified, and the crucial proteins were screeded from PPI network, including extracellular regulated protein kinases (ERK), AKT1, and tumor necrosis factor‐α (TNF). In addition, some key active components (palmatine, sexangularetin, and skullcapflavone II) were screened out from the active components‐targets network. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and in vivo experiments showed that protein‐serine‐threonine kinase (Akt)/MAPK pathway was involved in BXD treatment for UC; BXD and palmatine significantly ameliorated the severity of DSS‐induced UC in rats. Our study might assist in further investigation of the active components in Chinese medicine.

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Phytochemical analysis by HPLC-PDA/ESI-MS of Globularia alypum aqueous extract and mechanism of its protective effect on experimental colitis induced by acetic acid in rat

Cited by 17 publications

References 56 publications

Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal‐physiological function and against loperamide‐induced constipation coupled to oxidative stress and inflammation in rats

Purgative/laxative actions of Globularia alypum aqueous extract on gastrointestinal‐physiological function and against loperamide‐induced constipation coupled to oxidative stress and inflammation in rats

The potential of Thymus vulgaris aqueous extract to protect against delayed gastric emptying and colonic constipation in rats

Banxia Xiexin decoction ameliorates dextran sulfate sodium (DSS)‐induced ulcerative colitis via inhibiting serine‐threonine protein kinase (Akt)/mitogen‐activated protein kinase (MAPK) signaling pathway

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