Phytochemical analysis of Binahong (Anredera Cordifolia) leaves extract to inhibit In Vitro growth of Aeromonas Hydrophila

Basyuni, Mohammad; Ginting, Prita Yulianti Anasta Br; Lesmana, Indra

doi:10.1063/1.5011929

Cited by 13 publications

(23 citation statements)

References 8 publications

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“…The original habitat of this bacterium is the oral cavity. Other bacteria that can cause periodontal disease are Porphyromonas gingivalis, Prevotela intermedia, and Fusobacterium nucleatum which are a Gram-negative bacteria [6,17,18]. However, the most dominant bacteria found in periodontitis is the bacterium Aa [19].…”

Section: Discussionmentioning

confidence: 99%

“…Using a micropipette, the solution in each test tube 0.1 ml was taken and carried out spreading using a hockey stick on MHA media. All Petri dishes are inserted into the anaerobic jar and incubator for 24 h at 37°C as previously described by Basyuni et al [6]. After that done the calculation of colony and performed processing data analysis using Kruskal-Wallis test and continued by least significance different (LSD) test.…”

Section: Methodsmentioning

confidence: 99%

“…This plant showed the ability to inhibit the growth of cariogenic bacteria. Many active chemical compounds, especially terpenoids, are thought to have potential as an antibacterial, antidiabetic potentials, antihypertensive properties, antioxidative, and anticancer effects [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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The Effectiveness of Soursop Leaf Extract Against Growth of Aggregatibacter Actinomycetemcomitans Atcc® 6514™ in Vitro

Primasari

Nasution

Arbi

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC ® 6514™ growth. Methods:This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC ® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal-Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC ® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC ® 6514™ from each treatment group. Conclusion:Soursop leaf extract has antibacterial effectivity against Aa ATCC ® 6514 ™.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Effectiveness of Soursop Leaf Extract Against Growth of Aggregatibacter Actinomycetemcomitans Atcc® 6514™ in Vitro

Primasari

Nasution

Arbi

et al. 2018

Asian J Pharm Clin Res

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“…Lupeol and β-sitosterol (Basyuni et al 2017), and ursolic acid (Yuliani & Istyastono 2013) were detected by thin-layer chromatography (TLC). Using TLC as a support, UV-VIS, and FTIR spectrophotometry, Ekaviantiwi et al (2013) concluded that ethanolic extracts of leaves might contain p-coumaric acid.…”

Section: Phytochemistrymentioning

confidence: 99%

Ethnobotany, ecology, pharmacology, and chemistry of Anredera cordifolia (Basellaceae): a review

2020

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The potential of a plant with medicinal and nutritional properties, Anredera cordifolia, is reviewed. Its common names include “Bertalha” and “folha gorda” and it is popularly used for wound healing and against fungal infections and other types of infection. Its pharmacological properties have been widely investigated and acknowledged, especially with regard to its antibacterial activity, which improves the healing of wounds infected by Staphylococcus aureus, and to its antifungal activity against Candida albicans. It is an unconventional food plant, with leaves and aerial tubers used as food prepared in varied ways. It is also considered an invasive plant in several countries and thus classified as a weed. Its characteristics of a Brazilian native plant, with proven medicinal properties and unconventional use as food, underlie our study on its ecology and botanical classification, as well as the pharmacological assays and screening of chemical constituents.

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confidence: 99%

Impact of Solvent on the Characteristics of Standardized Binahong Leaf (Anredera cordifolia (Ten.) Steenis)

Dwitiyanti¹,

Harahap²,

Elya³

et al. 2019

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Binahong research, it contains several secondary metabolites of flavonoids, alkaloids, phenols, saponins, triterpenoids and sterols. 7 The methanolic extract of binahong leaves contains 8 glucopyranosyl-4 ', 5,7-trihydroxyprolavones used as blood glucose-lowering agents, 8 and triterpenoid saponin (boussingoside, momordin, ABSTRACT Background: Binahong is a plant that has the potential to be used as a traditional herbal medicine in Indonesia, and has several kinds of classes of compounds, one of them is a flavonoids glycosides (vitexin). Previous research reported that binahong leaves have pharmacological activities as antihyperglycemic, antihyperlipidemic, antibacterial, and others. A traditional plant that has proven efficacious needs to be standardized to ensure the quality and its safety. Objective: This study aimed to characteristics of binahong leaves simplicia obtained from Bogor, West Java. Materials and Methods: The crude extract was obtained by the maceration method using 40%, 70%, and 96% ethanol solvent. The selected extract then standardized, which includes macroscopic and microscopic observations and sets the standard parameter values binahong leaf extract. Parameters LCMS to identify active compounds semiquantitatively. Results: The yield of binahong ethanol extract from 40%, 70%, 96% showed a value of 10.9%, 11.4%, and 12.32%, respectively. From these results, 96% ethanol extract has proceeded for standardization. Macroscopic observation results showed that binahong leaves simplicia has a fine and notched form with 5-10 cm long and 3-7 cm diameter. The microscopic binahong leaves contain palisade tissue, parenchymal tissue, chlorophyll grains, rosette Caoxalate crystals, and spiral type. Phytochemical screening of binahong leaves showed the presence of alkaloids, flavonoids, saponins, tannins, steroids, and phenolic compounds.The standardization of binahong leaves ethanol extract down showed a levels of ethanol-soluble extract> 14.8%, water-soluble extract content > 13.5%, drying < 10%, water content < 8.9%, total ash content < 7.2%. LCMS profiles showed that ethanolic extract 40%, 70%, and 96% all contained vitexin at retention time 5.02 minutes, and m/z values 433.1111. Conclusion: 96% ethanolic extract of binahong leaves contains vitexin with pharmacognostic parameters carried out following the standards listed in the Indonesian herb pharmacopeia. She is expert in the area of Pharmacognosy and Phytochemistry, working in drug discovery of herbal plants, extraction technology, structure elucidation, and degenarative disease such as diabetes mellitus, antyhypertension, and cholesterol.Dwitiyanti, M.Farm., Apt.

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Phytochemical analysis of Binahong (Anredera Cordifolia) leaves extract to inhibit In Vitro growth of Aeromonas Hydrophila

Cited by 13 publications

References 8 publications

The Effectiveness of Soursop Leaf Extract Against Growth of Aggregatibacter Actinomycetemcomitans Atcc® 6514™ in Vitro

The Effectiveness of Soursop Leaf Extract Against Growth of Aggregatibacter Actinomycetemcomitans Atcc® 6514™ in Vitro

Ethnobotany, ecology, pharmacology, and chemistry of Anredera cordifolia (Basellaceae): a review

Impact of Solvent on the Characteristics of Standardized Binahong Leaf (Anredera cordifolia (Ten.) Steenis)

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