Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Saleh, Fatima; Darra, Nada El; Raafat, Karim; Ghazzawi, Iman El

doi:10.5530/pj.2018.1.18

Cited by 33 publications

(39 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The DPPH antioxidant capacity was found higher for methanolic extracts compared with the water extracts. Our findings are in agreement with previously reported results [ 55 , 56 ]. It is known that higher TPC content involves higher DPPH antioxidant activities as a result of the degree of hydroxylation in the phenolic compounds [ 57 ], as it was in our case when different extracts belonging to the same plant species were compared.…”

Section: Discussionsupporting

confidence: 94%

Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity

Pop

Bocşan

Chedea³

et al. 2020

Molecules

View full text Add to dashboard Cite

Natural products black cumin—Nigella sativa (N. sativa) and wild garlic—Allium ursinum (AU) are known for their potential role in reducing cardiovascular risk factors, including antracycline chemotherapy. Therefore, this study investigates the effect of N. sativa and AU water and methanolic extracts in a cellular model of doxorubicin (doxo)-induced cardiotoxicity. The extracts were characterized using Ultraviolet-visible (UV-VIS) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, Liquid Chromatography coupled with Mass Spectrometry (LC-MS) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) techniques. Antioxidant activity was evaluated on H9c2 cells. Cytosolic and mitochondrial reactive oxygen species (ROS) release was evaluated using 2′,7′-dichlorofluorescin-diacetate (DHCF-DA) and mitochondria-targeted superoxide indicator (MitoSOX red), respectively. Mitochondrial membrane depolarization was evaluated by flow cytometry. LC-MS analysis identified 12 and 10 phenolic compounds in NSS and AU extracts, respectively, with flavonols as predominant compounds. FT-IR analysis identified the presence of carbohydrates, amino acids and lipids in both plants. GC-MS identified the sulfur compounds in the AU water extract. N. sativa seeds (NSS) methanolic extract had the highest antioxidant activity reducing both intracellular and mitochondrial ROS release. All extracts (excepting AU methanolic extract) preserved H9c2 cells viability. None of the investigated plants affected the mitochondrial membrane depolarization. N. sativa and AU are important sources of bioactive compounds with increased antioxidant activities, requiring different extraction solvents to obtain the pharmacological effects.

show abstract

Section: Discussionsupporting

confidence: 94%

Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity

Pop

Bocşan

Chedea³

et al. 2020

Molecules

View full text Add to dashboard Cite

show abstract

“…Considering the richness of its biological heritage, it is possible that the seed extracts could contain one or more active ingredients that specifically target each disease. Indeed, the diversity of secondary metabolites [12][13][14] can explain this variety of therapeutic uses.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Profile and Antioxidant Activity of Nigella sativa L Growing in Morocco

Tiji

Benayad

Aziz

et al. 2021

The Scientific World Journal

View full text Add to dashboard Cite

Background. Nigella sativa L (NS) is a powerful antioxidant and medicinal plant with many therapeutic applications particularly in traditional medicine for respiratory, gastrointestinal, rheumatic, and inflammatory disorders, as well as cancer. Objective. The aim of this study is to extract the active ingredients from the Moroccan Nigella sativa L and determine its antioxidant properties. We hypothesize that the separation of the compounds from Nigella sativa L has either a positive or negative effect on antioxidants. To study this, we explored different methods to simultaneously extract and separate compounds from Nigella sativa L and performed antioxidant tests (β-carotene and DPPH) for all collected fractions. Methods. Nigella sativa L was hot-extracted by Soxhlet and mother extracts and was separated using silica column chromatography with adequate eluents. Qualitative phytochemical tests to determine the chemical families in Nigella sativa L seeds were performed on the fractions. They were also identified and characterized by GC-MS and HPLC-DAD. Then, antioxidant activity was examined by β-carotene bleaching and DPPH radical scavenger tests. Results and Conclusion. The mother extract hexane FH generated eight different fractions (SH1-8) and the acetone extract FA generated 11 fractions (SA1-11). The FH fractions had a high percentage of fatty acids, and the FA fractions had some interesting polyphenols derivative compounds. Phytochemical screening revealed secondary metabolites such as polyphenols flavonoids, alkaloids, steroids, terpenes coumarins, tannins, and saponins. We found that only two solvents (hexane, acetone) of different polarities could easily extract and simultaneously separate the components of Nigella sativa L. The antioxidant fractions that we collected had close activity to reference compounds but were more active than the corresponding mother extracts. Moreover, several IC50 values of fractions from acetone extract were better than those from hexane. Therefore, the antioxidant activity of Nigella sativa L is more attributed to flavonoids and polyphenols than fatty acids. In summary, the separation of hexane extract presents a more pronounced positive effect for antioxidant tests than acetone extract.

show abstract

“…Filter paper disc impregnated with DMSO was used as a negative control, while a standard disc containing gentamycin (10μg/disc) was used as positive control. All plates were incubated at 37°C for 18-24 h. After overnight incubation, the inhibition zones were recorded (Saleh et al, 2018).…”

Section: Agar Disc Diffusion Methodmentioning

confidence: 99%

Novel Method for The Valorization of Essential Oils From Orange By-Product

Samad¹,

Raafat²,

Khatib³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: A huge amount of citrus by-products are being wasted every year; these by-products can be used in the extraction of essential oils which can be used in different fields. Many methods have been used in the extraction of orange essential oil (E.O). This study focuses on the extraction of essential oil from orange by-product, using a newly developed extraction technique. This technique is a prototype combining a conventional method, namely, the hydrodistillation with the ultrasound-assisted extraction. Indeed, the traditional extraction methods of essential oils are time-consuming and expensive, moreover many organic compounds may decompose at high retained temperatures.Methods: a combination of traditional method with sonication improves efficiency and quality of the extraction, since ultrasonic extraction with precise and controlled operating parameters leads to higher extraction yields with minimum energy consumption. Therefore, the orange essential oil obtained using the prototype was compared to two commercial oils (CO1 and CO2) to create a valid comparison. Quality and safety tests have been performed, as well as bacterial load, antibacterial activities using the disc diffusion method, minimum inhibitory concentration (MIC), and prevention of bacterial biofilm formation.Results: As a result of extraction, the prototype method has shorten the extraction time, gave a higher quantity, and improved the extraction of essential oil from citrus peels without affecting oil composition when comparing it to hydrodistillation method. Conclusion: Orange E.O can be used as natural antibacterial agents to minimize bacterial growth, and inhibit biofilm formation by Staphylococcus aureus, Listeria monocytogenes and E.coli.

show abstract

Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria

Cited by 33 publications

References 27 publications

Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity

Evaluation of the Antioxidant Activity of Nigella sativa L. and Allium ursinum Extracts in a Cellular Model of Doxorubicin-Induced Cardiotoxicity

Phytochemical Profile and Antioxidant Activity of Nigella sativa L Growing in Morocco

Novel Method for The Valorization of Essential Oils From Orange By-Product

Contact Info

Product

Resources

About