Phytochemical analysis of Ocimum gratissimum by LC-ESI–MS/MS and its antioxidant and anxiolytic effects

Venuprasad, M. P.; Kandikattu, Hemanth Kumar; Razack, Sakina; Khanum, Farhath

doi:10.1016/j.sajb.2014.02.010

Cited by 53 publications

(42 citation statements)

References 48 publications

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“…Various scientific studies have shown that N. Laevis possesses antimicrobial, antidiabetic, antioxidant, and antimalarial activities (11)(12)(13)(14). O. gratissimum has been reported to show antidiabetic, antioxidant, anxiolytic, sedative, anti-inflammatory, hepatoprotective, antitumor, gastroprotective, hypolipidemic, insecticidal, nematicidal, and antibacterial activities (4,5,(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). Also, scientific reports have revealed that C. papaya possesses immunomodulatory, antimicrobial, anti-inflammatory, antihelmintic, anticancer, wound healing, anti-fertility, abortifacient, diuretic, antihypertensive and antimalarial properties (10,(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39).…”

Section: Introductionmentioning

confidence: 99%

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Eze

Nnanna

Okezie

et al. 2019

The EuroBiotech Journal

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Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of Newbouldia laevis, Ocimum gratissimum, and Carica papaya The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from N. laevis (NL-L1 and NL-L2), one from O. gratissimum (SL-L1), and two from C. papaya (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against S. aureus, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi C. albicans and A. fumigatus HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.

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Section: Introductionmentioning

confidence: 99%

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Eze

Nnanna

Okezie

et al. 2019

The EuroBiotech Journal

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“…9 Also, there are reports of the use of O. gratissimum to treat upper respiratory tract infections, pneumonia, conjunctivitis and skin diseases. 10 Various scientific studies have identified some of the plant's activities, such as antidiabetic, 11 antioxidant, 12 anxiolytic, 13 sedative, 14 antiinflammatory, 15 hepatoprotective, 16 antitumor, 17 gastroprotective, 18 and hypolipidemic. 19 The antibacterial activity of this plant species has been described in many studies that tested various parts of the plant: extract of leaves, 20 oil from leaves, 21 extract of leaves and stem, 22 oil from aerial parts, 23 and oil from leaves, stem and roots.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profile of Essential oil of Ocimum gratissimum L. and Evaluation of Antibacterial and Drug Resistance-modifying Activity by Gaseous Contact Method

Silva¹,

Carvalho²,

Matias³

2015

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Introduction: Plant products with antimicrobial potential and ability to modulate the action of drugs have become the target of several studies. Many studies of the species Ocimum gratissimum Lamiaceae have reportedbiological activities in the essential oil and extracts of various parts of the plant. Objective: Evaluate the antibacterial and resistance-modifying activity of the essential oil of O. gratissimum (OEOg) and its major compound, Eugenol. Materials and Methods: OEOg was extracted by hydrodistillation and Eugenol was obtained commercially. Multiresistant strains of Escherichia coli and Staphylococos aureus were used to test for drug activity. Antibacterial and resistance-modifying activity of OEOg and Eugenol was evaluated by gaseous contact. Results: Chemical analysis of OEOg demonstrated the presence of Eugenol and other compounds such as 1, 8-cineole and β-selinene as well. In the evaluation of the antibacterial activity, both OEOg and Eugenol were unable to form a bacterial growth inhibition zone at any of the concentrations tested. However, the modulation tests with amikacin and erythromycin identified synergism with OEOg and eugenol against E. coli and S. aureus. Gentamicin combined with OEOg showed antagonism against S. aureus but synergism against E.coli. When gentamicin was combined with Eugenol, there was also synergism against S. aureus, whereas no effect was seen against E. coli. Conclusion: Given the results obtained in this study, it is concluded that OEOg and Eugenol may eventually represent a new therapeutic tool against antibiotic-resistant bacteria.

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“…Aqueous leaf extract of OG also inhibited proliferation, migration, anchorage independent growth, morphogenesis, induction of COX-2 protein (Nangia-Makker et al ., 2007) and matrix metaloproteases (Nangia-Makker et al ., 2013) in breast cancer cells. Such tumor prevention potential is common in plants with polyphenolic compounds, anti-oxidants, vitamins, and v-3 fatty acids (Fahey et al ., 1997; Pezzuto, 1997; Kobayashi et al ., 2000); it is therefore not surprising that polyphenolic and antioxidant components were reported in OG (Venuprasad et al ., 2014). Hence, the anticancer activity of OG might have roles to play in its degenerative and deleterious effects on testicular cytoarchitecture and adverse changes in sperm parameters.…”

Section: Discussionmentioning

confidence: 95%

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Shittu

Olatunji

et al. 2019

JBRA Assisted Reproduction

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Objective:This study was designed to investigate the Ocimum gratissimum (OG) effects on sperm quality and testicular cytoarchitecture in alloxan-induced diabetic rats.Method:Twenty male Wistar rats (150-200 g) were assigned into 4 groups (n=5) as A (control), B (OG), C (Dia) and D (Dia+OG). Groups A and B were normal animals receiving distilled water or OG (400 mg/kg), respectively while diabetes was induced by alloxan monohydrate (100 mg/kg) in groups C and D, followed by the administration of distilled water or OG, respectively for 28 days. Blood samples were obtained for fasting blood glucose (FBG) and fructosamine determination while, epididymis and testes were obtained for sperm quality assessment using computer-assisted sperm analyzer and testicular histomorphometry, respectively. Seminiferous tubule diameter and interstitial space distance were quantified in hematoxylin and eosin stained slides. Statistical analysis was done using ANOVA and student t-test at α0.05.Results:Fructosamine and FBG were reduced in Dia+OG (80.11±3.80µmol/L and 132.0±8.41mg/dl, respectively) compared with Dia (139.66±4.29µmol/L and 285.6±26.69mg/dl, respectively). Sperm count was unchanged in Dia, but decreased in OG and Dia+OG; abnormal sperm cells increased in OG, Dia and Dia+OG. Mild vacuolation in the seminiferous tubule, disorganized germinal cells layer, arrested sperm maturation with empty spermatozoa in lumen, decreased seminiferous tubule diameter and increased interstitial space were found in the testes of OG, Dia and Dia+OG compared with control.Conclusion:Diabetes induces sperm impairments and distortions in testicular cytoarchitecture, which were aggravated by OG leaf extract in male Wistar rats.

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Phytochemical analysis of Ocimum gratissimum by LC-ESI–MS/MS and its antioxidant and anxiolytic effects

Cited by 53 publications

References 48 publications

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

Chemical Profile of Essential oil of Ocimum gratissimum L. and Evaluation of Antibacterial and Drug Resistance-modifying Activity by Gaseous Contact Method

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

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