Phytochemical Analysis of Two Cytotypes (2x and 4x) of Physalis angulata an Important Medicinal Plant, Collected from Rajasthan

Brar, Ramanpreet; Ch, Raghbir; Gupta, Vishal

doi:10.21767/2471-8084.100043

Biochem Mol biol J

2017

DOI: 10.21767/2471-8084.100043

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Phytochemical Analysis of Two Cytotypes (2x and 4x) of Physalis angulata an Important Medicinal Plant, Collected from Rajasthan

Ramanpreet Brar¹,

Raghbir Ch²,

Vishal Gupta³

Abstract: A simple, selective, sensitive and precise high performance thin layer chromatographic technique was followed to determine the diversity within two cytotypes of P. angulata. Fruit, stem, leaf and root samples extracted in n-hexane were used to quantify phenols and phytosterols and methanolic extracts for triterpinoids. The amounts of phytochemical differ in both the cytotypes of the same species.

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Cited by 3 publications

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“…The aim of present study is to find out new cytomorphotypes if any and to investigate these for future breeding experiments. Ploidy levels of the aforementioned Physalis species were examined, same results were recorded which were aligned with the various studies done in P. angulata 2n=24, 48 (Ganapathi et al 1991, Brar andGupta 2017), in P. peruviana 2n= 48, 72 (Shibata 1962, Panda andRao 1983) and in P. longifolia 2n= 48 (Tuteja and Bhatt 1984). There are few cyto-morphological studies has been done independently for all the Physalis species.…”

supporting

confidence: 65%

Withdrawal: Cyto-morphometric studies in three species of genus <i>Physalis</i> L. (Solanaceae) from North Western India

Singla,

Dhaliwal,

Sharma

2023

CYTOLOGIA

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One of a common commercial and culinary fruits from the Solanaceae family is the Cape gooseberry. Due to their enormous medicinal potential to treat a variety of ailments, the Physalis species have been employed traditionally. The Physalis species has a wide range of bioactivities based on the presence of secondary metabolites, including immunomodulatory, cytotoxic, anti-diabetic, antioxidant, anti-cholesterolemic, and antimicrobial. The plants gathered from various parts of Himachal Pradesh are subjected to meiotic exams in this study. The purpose of the current research was to study the ploidy levels of the aforementioned Physalis species using conventional chromosome counting techniques. More cytotypes, morphotypes, and chemotypes must be identified in order to conduct future breeding experiments for getting a crop with high medicinal content and stress tolerant variety. Therefore, morphological and meiotic analyses of three Indian Physalis species were performed. P. angulata L. (2n=4x=48) demonstrates a tetraploid nature, whereas P. peruviana L. (2n=2x=24) and P. longifolia Nutt. (2n=2x=24) are diploid.

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supporting

confidence: 65%

Withdrawal: Cyto-morphometric studies in three species of genus <i>Physalis</i> L. (Solanaceae) from North Western India

Singla,

Dhaliwal,

Sharma

2023

CYTOLOGIA

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Reproductive biology of Physalis angulata L. (Solanaceae)

Figueiredo

Passos

Hughes

et al. 2020

Scientia Horticulturae

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UPLC-DAD Assisted Phytochemical Quantitation Reveals a Sex, Ploidy and Ecogeography Specificity in the Expression Levels of Selected Secondary Metabolites in Medicinal Tinospora cordifolia: Implications for Elites’ Identification Program

Thakur

Rajpal

Raina

et al. 2020

CTMC

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Background: Medicinal phytochemistry involving UPLC-DAD in an exhaustive analysis involving quantification of eight commercially important phytochemicals viz. syringin, cordifolioside A, magnoflorine, tinocordiside, palmatine, 20β-hydroxyecdysone, L-tetrahydropalmatine and berberine has been done in 143 accessions from eight states and the union territories of Delhi and Jammu & Kashmir of India representing three different ploidy levels viz. diploid (2x), triploid (3x) and synthetic tetraploid (4x). The study was done to assess the effect of sex, ploidy level and ecogeography on the expression level of secondary metabolites in stems of dioecious, medicinally important shrub Tinospora cordifolia. Methods: Two different UPLC-DAD methods were used for the quantification of eight selected phytochemicals from the alcoholic stem extracts of T. cordifolia accessions. The Waters Acquity UPLC system hyphenated to the QTOF micromass system, equipped with PDA and ESI-Q-TOF detectors was utilized for the quantitative analysis, Mass Lynx v 4.0 software was used for data analysis. Results: Significant quantitative changes were observed in the analysed secondary metabolites among different accessions of T. cordifolia. The triploid (3x) cytotypes revealed higher amounts of seven out of eight analysed secondary metabolites than diploids and only 20β-hydroxyecdysone was observed to be present in significantly higher amount in diploid cytotypes. Further, at the tetraploid level, novel induced colchiploid (synthetic 4x) genotypes revealed increase in the yield of all of the analysed eight phytochemicals than their respective diploid counterparts. The quantity of active principles in tetraploid cytotypes were also higher than the average triploid levels at multiple locations in five out of eight tested phytochemicals, indicating the influence of ploidy on expression levels of secondary metabolites in T. cordifolia. Additionally, at each of the three ploidy levels (2x, 3x and synthetic 4x), a significant sex specificity could be observed in the expression levels of active principles, with female sex outperforming the male in the content of some phytochemicals, while others getting overexpressed in the male sex. The manifestation of diverse ecogeographies on secondary metabolism was observed in the form of identification of high yielding accessions from the states of Madhya Pradesh, Delhi and Himachal Pradesh and the Union territory of Jammu & Kashmir. Two triploid female accessions that contained approximately two- to eight fold higher amounts of five out of the eight analysed phytochemicals have been identified as superior elites from the wild from the states of Delhi and Madhya Pradesh. Conclusion: The paper shows the first observations of ploidy specificity along with subtle sex and ecogeography influence on the expression levels of secondary metabolome in T. cordifolia.

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Phytochemical Analysis of Two Cytotypes (2x and 4x) of Physalis angulata an Important Medicinal Plant, Collected from Rajasthan

Cited by 3 publications

References 14 publications

Withdrawal: Cyto-morphometric studies in three species of genus <i>Physalis</i> L. (Solanaceae) from North Western India

Withdrawal: Cyto-morphometric studies in three species of genus <i>Physalis</i> L. (Solanaceae) from North Western India

Reproductive biology of Physalis angulata L. (Solanaceae)

UPLC-DAD Assisted Phytochemical Quantitation Reveals a Sex, Ploidy and Ecogeography Specificity in the Expression Levels of Selected Secondary Metabolites in Medicinal Tinospora cordifolia: Implications for Elites’ Identification Program

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