Phytochemical and Antimicrobial Screening of Leaves and Stems of Balkan Endemic SpeciesDaphne MalyanaBlečić

Phytochemical, Antioxidant and Antimicrobial Profiles of Extracts of <i>Daphne alpina</i> (Thymelaeaceae) L Leaf and Twig from Mt Kopaonik (Serbia)

“…Our previous study on Daphne blagayana extracts showed higher content of flavonoids and phenols in twigs than leaf extracts [11,29,31] except endemic D. malyana [10]. These results can be explained by a similar chemical composition of the examined species Daphne dominated flavonoids and coumarins (daphnetin) which are the main carriers of biological activity in this genus.…”

“…About 40 years ago, it was found that mezerein of the species D. mezereum has antileukemic activity in the P -388 mouse lymphocytes [12]. Extracts of leaf and twigs of Daphne species showed antibacterial activity [9,10,[13][14][15]. However, to date there is still no large number of studies that suggest a wider application of biologically active compounds of this genus in medicine [16].…”

“…Analysis of the chemical characteristics of this genus show that plants of the genus Daphne contains many classes of secondary metabolites, some of which are dominated coumarins, flavonoids, lignans, and steroids [5][6][7][8][9][10][11]. Daphne species is used for the treatment of skin diseases, malaria, toothache, and also in traditional medicine as a purgative, anticoagulant and diuretic.…”

Antimicrobial activity and HPLC analysis of Daphne blagayana L. (Thymelaeceae) extracts

“…Comparing with previous assay of the antimicrobial activity of the methanol extracts of species D. malyana [21], in which the MIC values ranged from 2.75 to 22.0μg/mL, it can be concluded that D. blagayana having somewhat weaker antimicrobial activity.…”

Composition of the oily compounds, phytochemical screening and biological activity of different aerial parts of Smirnovia turkestana Bunge

“…The microplates were covered with lids; their contents were mixed well on a shaker operating at 300 rpm for 20 s, and then incubated statistically at appropriate temperature for 24 h. The appearance of white pellets on the bottom of wells was an evidence of microbial growth. Thus, 5 µL aliquots were taken from wells that did not show visible microbial growth and were spotted on plates containing NA medium, and incubated for 24 h. The lowest concentration of the plant extract which inhibited the growth of each microorganism was defined as MIC (Jusković et al 2012). All antimicrobial tests were repeated three times.…”