People rely on the synthetic chemical pesticides in most of the agricultural practices, which have a serious problem on human health and ecosystem. Essential oil (EO), derived from wild plants, offers a new opportunity to explore eco‐friendly green biocides. This study aimed to characterize the chemical constituents of EO from Egyptian ecospecies of Cleome droserifolia (Forssk.) Delile and evaluate its antioxidant and allelopathic potential. The EO was extracted from aerial parts by hydrodistillation and analyzed by GC/MS. To assess the allelopathic potential, EO concentrations from 50 to 200 μL L−1 were tested on Trifolium repens and three weeds. Antioxidant activity was determined using DPPH. Hydrodistillation yielded 0.64 % of dark yellow oil, which comprises 35 compounds, in which sesquiterpene was a major class and represented by 61.97 % of the total essential oil. Moreover, the dominant sesquiterpenes are cis‐nerolidol, α‐cadinol, δ‐cadinene, and γ‐muurolene. The speed of germination index, shoot and root length of clover and weeds were reduced in a concentration‐dependent manner. The IC50 values of C. droserifolia EO on germination of T. repens, Cuscuta trifolii, Melilotus indicus, and Chenopodium murale were 181.6, 183.5, 159.0, and 157.5 μL L−1, respectively. From the obtained data, we concluded that C. droserifolia EO could provide a hope to produce environment‐friendly bioherbicide as well as a natural resource of antioxidants.