Phytochemical and Cytotoxic Investigations of Pereskia grandifolia Haw. (Cactaceae) Leaves

Nurestri, A. M. S.; Sim, Kae Shin; Norhanom, A. W.

doi:10.3923/jbs.2009.488.493

Cited by 29 publications

(38 citation statements)

References 18 publications

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“…Sri Nurestri et al[51] reported that a mixture of methyl palmitate, methyl oleate and methyl stearate showed strong cytotoxic effect against Ca Ski, A549, as well as the normal cell line, MRC-5, with IC 50 values less than 20 ug/ml. Methyl palmitate was also reported to exert cytotoxic effect on T-cell leukemia cell line (Molt-4) with an IC 50 value of 2.28 ug/ml whilst methyl stearate showed cytotoxicity to acute promyeloblastic leukemia cell line (HL-60) and Molt-4 cell line with IC 50 values of 3.08 and 4.65 μg/ml respectively [52].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes

Phang

Malek

Ibrahim

2013

BMC Complement Altern Med

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BackgroundAlpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is used by the locals to treat flatulence. In this study, the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five different cancer and one normal cell lines were investigated. The total phenolic content of each extract and its fractions were also quantified. This is the first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract.MethodsIn the current study, the crude methanol and fractionated extract of the rhizomes of Alpinia pahangensis were investigated for their antioxidant activity using four different assays namely, the DPPH scavenging activity, superoxide anion scavenging, β-carotene bleaching and reducing power assays whilst their phenolic contents were measured by the Folin-Ciocalteu’s method.In vitro neutral red cytotoxicity assay was employed to evaluate the cytotoxic activity against five different cancer cell lines, colon cancer (HCT 116 and HT-29), cervical cancer (Ca Ski), breast cancer (MCF7) and lung cancer (A549) cell lines, and one normal cell line (MRC-5). The extract that showed high cytotoxic activity was further investigated for its chemical constituents by GC-MS (gas chromatography–mass spectrometry) analysis.ResultsThe ethyl acetate fraction showed the strongest DPPH radical scavenging (0.35 ± 0.094 mg/ml) and SOD activities (51.77 ± 4.9%) whilst the methanol extract showed the highest reducing power and also the strongest antioxidant activity in the β-carotene bleaching assays in comparison to other fractions. The highest phenolic content was found in the ethyl acetate fraction, followed by the crude methanol extract, hexane and water fractions. The results showed a positive correlation between total phenolic content with DPPH radical scavenging capacities and SOD activities. The hexane fraction showed potent cytotoxic effect against KB, Ca Ski and HCT 116 cell lines with IC50 of 5.8 ± 0.1 and 9.1 ± 2.0 ug/ml, respectively. The major components of hexane fraction analysed by GC-MS analysis were mostly methyl esters.ConclusionsThe current study suggests that the methanol extract and ethyl acetate fraction of A. pahangensis is a potential source of natural antioxidant for protective as well as prevention of life-threatening diseases. The hexane fraction of A. pahangensis may have the potential to be developed into therapeutic option for treating cancer.

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Section: Resultsmentioning

confidence: 99%

Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes

Phang

Malek

Ibrahim

2013

BMC Complement Altern Med

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“…A Pereskia bleo na Malásia é considerada uma planta medicinal com atividade antitumoral (ER et al, 2007;TURRA et al, 2007). Na Pereskia grandifolia, a vitamina E fitona e o 2,4-ditert-butilfenol (SRI NURESTRI et al, 2009) e na Pereskia bleo os compostos dihidroactinidiolide, 2,4-ditert-butilfenol, -tocopherol e fitol (SRI NURESTRI et al, 2008), apresentaram efeito citotóxico sobre as células carcinomas epidermoides nasofaríngea humana (KB), carcinoma cervical humano (CasKi), carcinoma do cólon humano (HCT116), carcinoma mamário dependente de hormônio (MCF7) e carcinoma de pulmão humano (A549). TAN et al (2005) observaram significante atividade citotóxica das folhas de Pereskia bleo sobre as linhagens T47D.…”

Section: Resultsunclassified

Utilização de cactáceas do gênero Pereskia na alimentação humana em um município de Minas Gerais

Almeida

Corrêa

2012

Cienc. Rural

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“…In Malaysia, they are traditionally used to treat cancer, high blood pressure, diabetes and diseases associated to rheumatism and inflammation. They are also used as remedy to relieve gastric pain, ulcer and as a body revitalizer (Sri Nurestri et al, 2009). Sahu et al (1974) indicated the use of Pereskia grandifolia to reduce swelling as the ones reported by Anon (1969).…”

Section: Introductionmentioning

confidence: 94%

“…Sri Nurestri et al (2009) reported an important cytotoxic activity against several strains of tumor cells utilizing crude extract from the leaves of this species. Sim et al (2010a) showed the antioxidant potential of ethyl acetate subfraction from methanolic extract of Pereskia grandifolia leaves, concluding that this cactus is a source of natural antioxidants.…”

Section: Introductionmentioning

confidence: 99%

“…Doetsch et al (1980) identified three alkaloids, p-methoxy-b-hydroxy-b-phenethylamine, 3-methoxytyramine and tyramine of Pereskia grandifolia leaves. Recent research of ethyl acetate fraction from methanolic extract resulted in the identification of some compounds: b-sitosterol, vitamin E, phytone, 2.4-di-tert-butylphenol and a mixture of 2.4-di-tert-butylphenol, methyl palmitate, methyl oleate and methyl estearate (Sri Nurestri et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)

Kazama

Uchida

Canzi

et al. 2012

Journal of Ethnopharmacology

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Phytochemical and Cytotoxic Investigations of Pereskia grandifolia Haw. (Cactaceae) Leaves

Cited by 29 publications

References 18 publications

Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes

Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes

Utilização de cactáceas do gênero Pereskia na alimentação humana em um município de Minas Gerais

Involvement of arginine-vasopressin in the diuretic and hypotensive effects of Pereskia grandifolia Haw. (Cactaceae)

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