Phytochemical and efficacy study on four herbs used in erectile dysfunction: Mondia whiteii, Cola acuminata, Urtica massaica, and Tarenna graveolens

Oloro, Joseph; Kihdze, Tanayen Julius; Katusiime, Barbra; Imanirampa, Lawrence; Waako, Paul; Bajunirwe, Francis; Ganafa, Amon Agaba

doi:10.5897/ajpp2015.4405

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…The Fe 3+ reducing powers of the three MWL extracts were determined as previously reported with slight modification [34,42]. Different concentrations (25-100 μg/ml) of the extracts (1.0 ml) were mixed with 2.5 ml phosphate buffer (pH 7.0) and 2.5 ml potassium ferricyanide (1%), followed by incubation at 50 °C for 30 min.…”

Section: Ferric Reducing Power Activitymentioning

confidence: 99%

Elucidation of chemical profiles and molecular targets of Mondia whitei leave fractions bioactive as novel therapeutics: an in vitro and in silico assay

Onohuean

Igbinoba

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Mondia whitei root is often used in Africa as a local therapeutic agent for libido enhancement. The fractions of the M. whitei leaves (MWL) lack chemical characterization of their bioactive components and possible molecular targets. We characterized and investigated its molecular target as therapeutic agents in an in vitro and in silico assay. Mineral compositions, antioxidant, and GC-MS characterization were studied. The cytotoxicity effect was measured on HeLa and HT-29 cells by MTT assay. In silico potential inhibitors of Cathepsin B (CathB) as a cancer biomarker were determined. Results The flame photometry produced marked Na+ and K+. GC-MS revealed eighteen bioactive components. The fractions (chloroformic 47.00, ethanolic 45.52, and aqueous 40.13) of MWL caused a higher inhibition ratio compared to standards. The MWL showed a significant cytotoxic effect on the treated cell lines at concentrations of 150 and 200 μg/ml and 100, 150, and 200 μg/ml for HT-29 and HeLa cells, respectively. Ten bioactives (MWL 4, 5, 6, 8, 9, 10, 14, 15, 17, and 18) showed potential inhibition of CathB with binding affinities of −4.40 to −8.3 Kcal/Mol. However, MWL 4, 9, 14, and 17 which have higher binding affinities (−6.7, −7.1, −8.2, and −8.3, respectively) than the standard inhibitor (−6.5) were the lead molecules. Conclusion These chemical profiles and potential molecular targets unraveled in this study propose that MWL has a promising anticancer activity. Graphical Abstract

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Section: Ferric Reducing Power Activitymentioning

confidence: 99%

Elucidation of chemical profiles and molecular targets of Mondia whitei leave fractions bioactive as novel therapeutics: an in vitro and in silico assay

Onohuean

Igbinoba

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…Thus, several plants are used for this purpose, such as Garcinia cola 12 , Mondia whitei 13 , Withania somnifera, Asphaltum, Tribulus terrestris, Asparagus racemosus and Sida cordifolia 5 . Carissa spinarum is one of the plants used in the West African sub-region, especially in Togo to manage erectile dysfunction 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative Phytochemical Screening, Antioxidant and Aphrodisiac Potential Studies of Hydroethanolic Root Extract of Carissa spicatum L.

Mawubédjro,

Diallo,

Lawson-Evi

et al. 2023

Asian J. Biol. Sci

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Background and Objective: Carissa spinarum is a plant used in West African countries such as Togo to manage erectile dysfunction. Despite this usage, there are no scientific proof of its aphrodisiac potential. This work was then, carried out to evaluate the aphrodisiac potential of Carissa spinarum roots hydro-alcoholic extract associated with its quantitative phytochemical composition and its antioxidant activity. Materials and Methods: Prior to the aphrodisiac potential study, the quantitative phytochemical screening and the antioxidant activity of the extract were assessed. The quantitative phytochemical screening was done to determine the amount of tannin, total phenols, flavonoids and cardiac glycosides in the extract. The antioxidant properties were performed through total antioxidant capacity (TAC), reducing power and, free radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH) eliminating activity. The aphrodisiac potential of the extract was evaluated by studying its effect (75 and 150 mg kgG 1 ) on sexual behavior (number of sexual rises and anogenital sniffing as well as their respective latency) in male ICR mice compared to sildenafil (5 mg kgG 1 ). Results: The hydroethanolic root extract of Carissa spinarum contained 23.21±1.42 mg Eq AG/g of extract of total phenol, 14.97±0.30 mg Eq of rutin/g of extract of flavonoids, 7.09±1.42 mg of Eq AG/g of extract of tannins and 7.84±0.42 mg d'Eq of digoxin/g of extract of cardiac glycosides. The extract produced antioxidant activity and significantly altered sexual behavior parameters, especially at the dose of 150 mg kgG 1 . Thus, the number of sexual mounting and anogenital sniffing was significantly increased (p<0.0001). Inversely, their respective latency decreased significantly (p<0.0001). Conclusion: It emerges from this study, that the hydroethanolic extract of Carissa spinarum roots has antioxidant properties and aphrodisiac activities.

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“…Toxins can be taken through ingestion or created by body physiological reactions (Olaniyan et al, 2016). Regarding their roles, the liver and the kidney are vital organs used as toxicity indices for evaluating the toxicity of pharmaceutical drugs and plant extracts (Joseph et al, 2015;Olaniyan et al, 2016). For instance, since the liver and the kidney are detoxifying organs, they are the first targets of toxicants and their adverse effects such as degenerations and alterations (Bello et al, 2016).…”

Section: Toxicity Evaluationmentioning

confidence: 99%

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

Hanolo

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Infectious diseases such as fungal diseases are the global health problem. Moreover, antimicrobial resistance to modern fungicides and antibiotics is even perplexing in the health settings. Such situation needs serious attention to search for alternative antimicrobial agents. Thus, this study aimed to investigate antimicrobial activity of Tephrosia vogelii Hook.f found in Tanzania and develop antifungal agents therefrom. The n-hexane, dichloromethane and methanolic extracts from leaves and roots of Tephrosia vogelii were evaluated for antimicrobial activity against Candida albicans (ATCC 90028), Cryptococcus neoformans (clinical isolate), Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC29953), Klebsiella pneumoniae (ATCC 700603) and Salmonella typhi (NCTC 8385). A serial dilution method using the sterilized 96 wells of polystyrene microliter plates were used to determine the minimum inhibitory concentrations (MIC) of extracts. The in vivo toxicity of methanolic leaf and root extracts was evaluated using albino rats. Extracts doses of 600, 1200, 2000 and 5000 mg/kg body weight were evaluated for lethality test while doses of 600, 1200 and 2000 mg/kg body weight subjected for sub-acute toxicity test. Gas Chromatography-Mass Spectrometry (GC-MS) technique was employed to appraise phytochemical compounds present. Antifungal agent for topical application was formulated and evaluated through in vitro and in vivo test to establish its effectiveness. The study results revealed that n-hexane and dichloromethane extracts exhibited the lowest activity. Equally, methanolic root and leaf extracts exhibited high activity at MICs ranging from 0.625 - 5.0 mg/mL against assayed pathogens. Phytochemical screening of both methanolic leaf and root extracts revealed the presence of tannins, flavonoids, terpenes, fatty acids and saponins. The GC-MS analysis unveiled the presence of ten phytocompounds. Five compounds; demethylmunduserone sumatrol, munduserone, hexadecanoic acid and hexadecanoic acid, methyl ester are new and this is the first time to be reported from Tephrosia vogelii. Furthermore, toxicity evaluation revealed that methanolic leaf and root extracts of Tephrosia vogelii were not deleterious at highest dose of 5000 mg/kg body weight. Though there was no mortality recorded, histopathological examination revealed toxicity indices due to vacuolation and necrosis in hepatocytes under treatment of methanolic root extracts of dose 2000 mg/kg body weight. The herbal cream prototype (CBTV3) was formulated, and it exhibited antimicrobial activity through in vitro and in vivo tests. The development of herbal cream prototype from Tephrosia vogelii Hook.f is hereby reported for the first time.

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Phytochemical and efficacy study on four herbs used in erectile dysfunction: Mondia whiteii, Cola acuminata, Urtica massaica, and Tarenna graveolens

Cited by 8 publications

References 10 publications

Elucidation of chemical profiles and molecular targets of Mondia whitei leave fractions bioactive as novel therapeutics: an in vitro and in silico assay

Elucidation of chemical profiles and molecular targets of Mondia whitei leave fractions bioactive as novel therapeutics: an in vitro and in silico assay

Quantitative Phytochemical Screening, Antioxidant and Aphrodisiac Potential Studies of Hydroethanolic Root Extract of Carissa spicatum L.

Antimicrobial investigation of Tephrosia Vogelii hook.f from Hai District in Tanzania towards development of antifungal agents for topical application

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