Phytochemical and pharmacological potential of<i>Medicago sativa</i>: A review

Bora, Kundan Singh; Sharma, Anupam

doi:10.3109/13880209.2010.504732

Cited by 143 publications

(97 citation statements)

References 61 publications

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“…It has been used historically as a medicine to treat coughs and digestive problems and is used as a dietary supplement in different forms (tablets, powders, and tea) to benefit both humans and animals [1]. Numerous reports have proved that alfalfa has immunostimulatory, antioxidant, pharmacological, and antidiabetic activity [2,3], which are mainly attributed to the phytochemicals derived from the plant such as flavonoids [4], polysaccharides [5], and saponins [6].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

2015

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Ultrasonic-assisted extraction (UAE) was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology (RSM), based on a four-factor, five-level central composite design (CCD), was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) methods. The results showed good fit with the proposed models for the total flavonoid extraction (R 2 = 0.9849), for the antioxidant extraction assayed by ABTS method (R 2 = 0.9764), and by DPPH method (R 2 = 0.9806). Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL/g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL/g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL/g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa.

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Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

2015

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“…It is one of the oldest plants used as fodder and one of the most cultivated legumes, with production reaching 436 million tons in the US alone. [1][2][3] M. sativa contains a variety of phytochemicals, including phenolic and other antioxidants. [2,4] The value of natural antioxidants in food and medicinal plants is now widely recognized, particularly due to their role in the prevention of degenerative disorders such as cancer and cardiovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] M. sativa contains a variety of phytochemicals, including phenolic and other antioxidants. [2,4] The value of natural antioxidants in food and medicinal plants is now widely recognized, particularly due to their role in the prevention of degenerative disorders such as cancer and cardiovascular diseases. [5] Alfalfa contains also some specific classes of phenolic compounds that have distinct pharmacological features.…”

Section: Introductionmentioning

confidence: 99%

“…[6] They have demonstrated estrogenic [7] and apoptotic activity, [8] and certainly contribute to many of the numerous observed pharmacological properties of M. sativa. [1,2,4] Ultrasonic-assisted extraction (UAE) is an inexpensive, rapid, simple and efficient technique for extraction, most often used for solid/liquid systems. In UAE, transmission of mechanical waves through a fluid causes oscillation of the molecules around their equilibrium position.…”

Section: Introductionmentioning

confidence: 99%

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Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

2017

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Response surface methodology was used to optimize experimental conditions for ultrasound-assisted extraction of functional components from Medicago sativa L. (Fabaceae) aerial parts. The procedure was performed according to the Box-Behnken design. The independent variables were ethanol concentration (X1), temperature (X2), and pH (X3), while the responses were extraction yield, DPPH radical scavenging activity of the extracts (RSA IC50), content of total phenols (TP), total flavonoids (TF) and total phenolic acids (TPA). The response values of the extracts prepared at optimum conditions (X1, X2, X3) were: yield (162 mg/ml, 20 %, 60 °C, 5.5), TP (6.63 mg/ml, 100 %, 60 °C, 5.5), TF (0.292 mg/ml, 100 %, 23 °C, 5.5), TPA (0.275 mg/ml, 100 %, 23 °C, 8.5) and RSA IC50 (3.97 mg/ml, 40 %, 60 °C, 7.0). The predicted values matched well with the observed ones indicating the suitability of the selected models for extraction of bioactive compounds from M. sativa.

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“…Alfalfa (Medicago sativa L., family Fabaceae) is a perennial herb with seeds that have long been used in traditional medicine for prevention and cure of various ailments [5]. However and up to our knowledge, few studies scrutinizing the antimicrobial capacity of alfalfa vegetative parts can be recorded [6].…”

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Economic Maximization of Alfalfa Antimicrobial Efficacy Using Stressful Factors

Mickky

Abbas

El-Shhaby

2016

Int J Pharm Pharm Sci

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Objective: The present study addresses the effect of water deficit stress on the antimicrobial capacity of alfalfa (Medicago sativa) plants.Methods: Methanolic extracts of alfalfa plants grown in different soil types, varying in sand proportion, either alone or combined with various levels of water regimes were assessed for antibacterial and antifungal activities following cup plate method. The phytochemical profiles of plant extracts were also qualitatively screened using appropriate chemical reagents. Moreover, data were intensively processed via two different statistical designs. For centuries, desertification and drought could affect many parts of the world with remarkable negative impact on land productivity. Almost every year, new sectors of the earth are hit by theses stresses bringing about paramount disturbance in agricultural yield [1]. Therefore, many studies have been registered to estimate the consequences of such constraints on plant performance, the response of plants to these devastating factors and also the techniques that could be employed to ameliorate their adverse effects. Nevertheless, it is to somewhat scarce to find studies on how to take advantage from these stressful conditions that threaten vast tracts of ecosystems. ResultsFor a known medicinal plant species, certain molecules with pharmacological activity may be over-synthesized when it is grown under a new set of environmental conditions, and this would elucidate the improvement of its beneficial effects under stress. In this connection, the levels of bioactive phytochemicals were documented to fluctuate with the environmental conditions [2]. Generally, comparative analyses manifested that the content of various secondary metabolites in plants suffering water shortage is higher than that in their synonyms growing under optimum circumstances [3].The failure of some available antibiotics produced by pharmacological industries to combat some pathogenic microbes, along with the baleful side effects of antibiotic overuse and misuse have forced researchers to investigate the antimicrobial activity of medicinal plants [4]. Alfalfa (Medicago sativa L., family Fabaceae) is a perennial herb with seeds that have long been used in traditional medicine for prevention and cure of various ailments [5]. However and up to our knowledge, few studies scrutinizing the antimicrobial capacity of alfalfa vegetative parts can be recorded [6].Therefore, the present study aims at exploring stress utilization as an effectual simple, low-cost and low-risk strategy to promote alfalfa efficacy as a medicinal plant. In a trial to maximize its antimicrobial efficiency, alfalfa plants would be grown with little water supply employed to different levels either alone or combined with elevated sand proportion in the growing soil.Seeds of alfalfa (Medicago sativa L., cultivar Nubaria 1) were obtained from Al Nubaria Agricultural Organization, El Biheera Governorate, Egypt. The seeds were sown in 3 groups of pots packed with clay and sand mixed to obtain 3 type...

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Phytochemical and pharmacological potential ofMedicago sativa: A review

Cited by 143 publications

References 61 publications

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

Economic Maximization of Alfalfa Antimicrobial Efficacy Using Stressful Factors

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