Phytochemical and Toxicological Studies of Methanol and Chloroform Fractions of Acanthus montanus Leaves

Parker, Joshua Elijah; Ukegbu, Chimere Young; Chinenye, Nwike P.; Uzoma, Ikpe; Echezona, Ezeani; Innocen, Orhonigbe O.; Paul, Nweje-Anyalowu C.

doi:10.3923/jbs.2021.52.58

Cited by 2 publications

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“…The extraction yield of the plant extracts was determined according to the following formula [20]: R (%) = (W / W0) × 100 R: extraction yield (%), W: weight (g) of dry extract, W0: weight (g) of the crushed plant. The two dried extracts after lyophilization were kept in a freezer until used for the biological tests.…”

Section: Preparation Of the Plant Extractsmentioning

confidence: 99%

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Belem-Kabré,

Nitièma,

Odjo

et al. 2023

Preprint

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Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods. The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A2 was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The extracts demonstrated the ability to reduce oxidative stress. The hydroethanolic extract displayed the most significant anti-lipoxygenase activity with an IC50 value of 55.82 ± 1.15 µg/mL. The lethal dose (LD50) of extracts was estimated to be superior to 2000 mg/kg bw. The ability of the samples to produce one cm amplitude of contraction at 50% effective concentrations (EC50) was 1.51 ± 0.08 mg/mL for aqueous extract and 3.57 ± 0.61 mg/mL for hydroethanolic extract. These findings could provide evidence for using Anastatica hierochuntica to augment labor.

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Section: Preparation Of the Plant Extractsmentioning

confidence: 99%

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Belem-Kabré,

Nitièma,

Odjo

et al. 2023

Preprint

View full text Add to dashboard Cite

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Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of Anastatica hierochuntica L. (Brassicaceae) on the Isolated Uterus of Mice

Esther,

Mathieu,

Saïdath

et al. 2023

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Background: Anastatica hierochuntica L. (Brassicaceae) has been claimed to treat various conditions, including complicated childbirth. The principal objective of this research was to provide scientific evidence on the contractile effect of the aqueous and hydroethanolic extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative and quantitative methods helped identify and quantify the phytoconstituents in both extracts. The antioxidant activity was studied using four standard methods (ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase A 2 was determined. The contractile effects of the extracts were evaluated using isolated pregnant mice uteri. Steroids, triterpenoids, flavonoids, tannins, coumarins, and reduced compounds were identified in both extracts. The hydroethanolic extract exhibited higher content of hydrolyzable tannins (15.07 ± 0.24 mg tannic acid equivalent/g) than the aqueous decoction (11.8 ± 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation.

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Phytochemical and Toxicological Studies of Methanol and Chloroform Fractions of Acanthus montanus Leaves

Cited by 2 publications

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Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of <em>Anastatica hierochuntica</em> L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of <em>Anastatica hierochuntica</em> L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of <i>Anastatica hierochuntica</i> L. (Brassicaceae) on the Isolated Uterus of Mice

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Phytochemical and Toxicological Studies of Methanol and Chloroform Fractions of Acanthus montanus Leaves

Cited by 2 publications

References 0 publications

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of <em>Anastatica hierochuntica</em> L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Phytochemical Screening and Evaluation of Contractile Effects of the Aqueous and Hydroethanolic Extracts of <em>Anastatica hierochuntica</em> L. (Brassicaceae) on Pregnant Mice Isolated Myometrium

Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of &lt;i&gt;Anastatica hierochuntica&lt;/i&gt; L. (Brassicaceae) on the Isolated Uterus of Mice

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Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of <i>Anastatica hierochuntica</i> L. (Brassicaceae) on the Isolated Uterus of Mice