Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution

Sangkanu, Suthinee; Mitsuwan, Watcharapong; Mahboob, Tabassum; Mahabusarakam, Wilawan; Chewchanwuttiwong, Sopa; Siphakdi, Panisara; Jimoh, Tajudeen O.; Wilairatana, Polrat; Dolma, Karma G.; Pereira, Maria de Lourdes; Rahmatullah, Mohammed; Wiart, Christophe; Norouzi, Roghayeh; Siyadatpanah, Abolghasem; Mutombo, Polydor Ngoy; Nissapatorn, Veeranoot

doi:10.1016/j.actatropica.2021.106266

Cited by 12 publications

(9 citation statements)

References 38 publications

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“…The highest adhesion was noticed in the control group, which was an untreated agent. Similar results were obtained in the current study, where anti-adhesion was observed in plastic plate and contact lenes belonging to Curcuma longa extract 69 , Annona muricata and Combretum trifoliatum extracts 70 , and Garcinia mangostana and their pure compounds 13 .…”

Section: Discussionsupporting

confidence: 91%

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Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects

Sama‐ae

Sangkanu²,

Siyadatpanah³

et al. 2022

F1000Res

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Background: Propolis is a natural resinous mixture produced by bees. It provides beneficial effects on human health in the treatment/management of many diseases. The present study was performed to demonstrate the anti-Acanthamoeba activity of ethanolic extracts of Propolis samples from Iran. The interactions of the compounds and essential proteins of Acanthamoeba were also visualized through docking simulation. Methods: The minimal inhibitory concentrations (MICs) of Propolis extract against Acanthamoeba trophozoites and cysts was determined in vitro. In addition, two-fold dilutions of each of the agents were tested for encystment, excystment and adhesion inhibitions. Three major compounds of Propolis extract such as chrysin, tectochrysin and pinocembrin have been selected in molecular docking approach to predict the compounds that might be responsible for encystment, excystment and adhesion inhibitions of A. castellanii. Furthermore, to confirm the docking results, molecular dynamics (MD) simulations were also carried out for the most promising two ligand-pocket complexes from docking studies. Results: The minimal inhibitory concentrations (MICs) 62.5 and 125 µg/mL of the most active Propolis extract were assessed in trophozoites stage of Acanthamoeba castellanii ATCC30010 and ATCC50739, respectively. At concentrations lower than their MICs values (1/16 MIC), Propolis extract revealed inhibition of encystation. However, at 1/2 MIC, it showed a potential inhibition of excystation and anti-adhesion. The molecular docking and dynamic simulation revealed the potential capability of Pinocembrin to form hydrogen bonds with A. castellanii Sir2 family protein (AcSir2), an encystation protein of high relevance for this process in Acanthamoeba. Conclusions: The results obtained provided a candidate for the development of therapeutic drugs against Acanthamoeba infection. In vivo experiments and clinical trials are necessary to support this claim.

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Section: Discussionsupporting

confidence: 91%

“…The anti-adhesion assay was modified as previously reported 13 . Trophozoites (4 × 10 5 cells/mL) were added to each well of a 96-well polystyrene microtiter plate supplemented with 1/2 MIC, 1/4 MIC, 1/8 MIC, 1/16 MIC of Propolis extract.…”

Section: Anti-adhesion On Acanthamoeba Castellaniimentioning

confidence: 99%

Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects

Sama‐ae

Sangkanu²,

Siyadatpanah³

et al. 2022

F1000Res

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“…The viability of A. triangularis trophozoites and cysts was determined by trypan blue exclusion staining. A. triangularis suspension was mixed with the same volume of 0.2% trypan blue and incubated at ambient temperature for 3 min ( Sangkanu et al, 2022 ). The viable (unstained) and dead (stained dark blue) amoebae were enumerated using a hemocytometer (Tiefe Depth Profondeur, Marienfeld, Germany) under an inverted microscope (Nikon ECLIPSE TE2000-S, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…Mangosteen pericarp (MP) is an important source of bioactive xanthones, which has remarkable antioxidant, anti-inflammatory, immunomodulatory, antiviral, antifungal, antibacterial, and anticancer activities ( Abate et al, 2022 ; Nauman & Johnson, 2022 ). In literature, G. mangostana extract and α-mangostin have previously shown anti- Acanthamoeba activity and anti-adhesion properties against Acanthamoeba triangularis ( Sangkanu et al, 2021 ; Sangkanu et al, 2022 ). In this study, mangostin isolated from the pericarps of G. mangostana was modified to three semi-synthetic derivatives using epoxidation and nitrile-addition reactions.…”

Section: Introductionmentioning

confidence: 99%

Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens

Chuprom

Sangkanu

Mitsuwan

et al. 2022

PeerJ

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Garcinia mangostana L., also known as the mangosteen tree, is a native medicinal plant in Southeast Asia having a wide variety of pharmacologically active compounds, including xanthonoid mangostin. In this study, we examined the pharmacological activities of the selected semi-synthetic mangostin derivative, namely, amoebicidal activity, encystation inhibition, excystation activity, and removal capacity of adhesive Acanthamoeba from the surface of contact lens (CL). Among the three derivatives, C1 exhibited promising anti-Acanthamoeba activity against Acanthamoeba triangularis WU19001 trophozoites and cysts. SEM images displayed morphological changes in Acanthamoeba trophozoites, including the loss of acanthopodia, pore formation in the cell membrane, and membrane damage. In addition, the treated cyst was shrunken and adopted an irregular flat cyst shape. Under a fluorescence microscope, acridine orange and propidium iodide (AO/PI) staining revealed C1 induced condensation of cytoplasm and chromatin with the loss of cell volume in the treated trophozoites, while calcofluor white staining demonstrated the leakage of cell wall in treated cysts, leading to cell death. Interestingly, at the concentration ranges in which C1 showed the anti-Acanthamoeba effects (IC50 values ranging from 0.035–0.056 mg/mL), they were not toxic to Vero cells. C1 displayed the highest inhibitory effect on A. triangularis encystation at 1/16×MIC value (0.004 mg/mL). While C1 demonstrated the excystation activity at 1/128×MIC value with a high rate of 89.47%. Furthermore, C1 exhibited the removal capacity of adhesive Acanthamoeba from the surface of CL comparable with commercial multipurpose solutions (MPSs). Based on the results obtained, C1 may be a promising lead agent to develop a therapeutic for the treatment of Acanthamoeba infections and disinfectant solutions for CL.

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“…Antimicrobial topical treatments are available for AK infection and usually comprise a combination of propamidine isethionate and neomycin or chlorhexidine, while contact lens disinfectants often include chlorhexidine [ 2 ]. Notably, a plethora of studies have shown that many of the contact lens disinfectants commercially available in the market are ineffective against Acanthamoeba [ 9 , 10 ], suggesting the need to identify the compound(s) that are biologically relevant, cost effective, effective against cysts and trophozoites, and nontoxic, and which can be used as disinfectants and/or therapeutic agents [ 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Plant-Based Metabolites as Disinfectants against Acanthamoeba castellanii

et al. 2022

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Due to global warming, coupled with global water shortages and the reliance of the public on household water tanks, especially in developing countries, it is anticipated that infections caused by free-living amoebae such as Acanthamoeba will rise. Thus, the development of novel disinfectant(s) which can target pathogenic free-living amoebae effectively is warranted. Herein, we extracted and isolated several plant-based secondary metabolites as novel disinfectants for use against pathogenic Acanthamoeba. The identity of the compounds was confirmed by nuclear magnetic resonance and tested for antiamoebic activities against clinical isolate of A. castellanii, belonging to the T4 genotype. Amoebicidal assays revealed that the compounds tested showed antiamoebic properties. Betulinic acid and betulin exhibited parasite killing of more than 65%. When tested against the cyst stage, betulinic acid, betulin, and vanillic acid inhibited both encystation and excystation processes. Furthermore, the plant-based metabolites significantly inhibited the binding capability of A. castellanii to host cells. Finally, most of the tested compounds displayed minimal cytotoxic activities against human cells and noticeably perturbed amoeba-mediated host cell cytotoxicity. Notably, both alkaloid and betulinic acid showed 20% cytotoxic effects, whereas betulin and lupeol had cytotoxic effects of 24% and 30%, respectively. Overall, our findings indicate that plant-based natural compounds demonstrate anti-Acanthamoebic properties, and they have potential candidates for water disinfectants or contact lens disinfecting solutions, as well as possible therapeutic drugs against Acanthamoeba infections.

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Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution

Cited by 12 publications

References 38 publications

Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects

Targeting Acanthamoeba proteins interaction with flavonoids of Propolis extract by in vitro and in silico studies for promising therapeutic effects

Anti-Acanthamoeba activity of a semi-synthetic mangostin derivative and its ability in removal of Acanthamoeba triangularis WU19001 on contact lens

Novel Plant-Based Metabolites as Disinfectants against Acanthamoeba castellanii

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