Phytochemical components and GC–MS analysis of Petiveria alliaceae L. fractions and volatile oils

Abstract

Phytochemical constituents are important in the determination of plant activities. Their presence in medicinal plants gives their therapeutic values. These phytoconstituents possesses pharmacological activities that include antimicrobial, antioxidant, anti-inflammatory, analgesics, anti-diabetic, anti-hypertensive, antidiarrheal and several other activities. These activities can be determined by the identification of the phytochemicals present in medicinal plants. Petivera alliaceae L. is one of the medicinal plants in the family of phtytolaccaceae used traditionally as an antirheumatic, analgesics, antimicrobial, anticancer and immunomodulators. It is believed to possess rich phytoconstituents especially sulphur containing a broad-spectrum antimicrobial activity. Although the root extracts is been explored but there are limited publication to its root fractions. Therefore, the study is aimed at screening phytochemicals present in the fractions and volatile oil of the root parts of P. alliaceae using qualitative tests and Gas Chromatography–Mass Spectrometry (GC–MS) analysis. Fresh root parts of Petivera allliaceae plant were collected and air-dried. The dried root parts was macerated in absolute methanol for 72 h. The solution was extracted and dried. The dried root methanol extract was partitioned into n-hexane (PAH) and methanol fractions (PAM), while volatile oil (PAO) was extracted using Clevenger-type hydrodistillation method. The result shows that alkaloids, saponins and flavonoids were present while cardiac glycosides, phenol, terpenoids and anthraqunione glycosides were absent in both fractions. The GC–MS analysis identified 18 compounds in PAH, 19 compounds in PAM and 28 compounds in PAO. The fractions and volatile oils of P. alliaceae roots are rich in phytochemical constituents and compounds should be isolated from the fractions and explored for their potentials.

Ethnobotany, chemistry and toxicity of Petivera alliacea: A review

“…alliacea leaves are dried and ground into powder, which is used as herbal preparations such as teas, extracts, and capsules purposely to boost the immune system and increase metabolic processes in humans [23] Phytochemistry: Scientists looked at the phytochemicals in P. alliacea's leaf, stem bark, and root and found alkaloids, steroids, glycosides, flavonoids, terpenoids, and tannins [22]. P. alliacea hexane and methanol fractions and volatile oils were subjected to GC-MS analysis as well as phytochemical component analysis in a different study [23]. Hexane and methanol fractions included alkaloids, saponins, and flavonoids, but not cardiac glycosides, phenols, terpenoids, or anthraquinone glycosides.…”

“…The P. alliacea hexane fraction included 18 compounds, the P. alliacea methanol fraction contained 19 compounds, and the volatile oils contained 28 compounds. These findings were obtained using GC-MS analysis [23].…”

Antioxidant Properties and Secondary Metabolites Profile of Hyptis colombiana at Various Phenological Stages

“…The antioxidant properties of EOs and extracts obtained from plants depend on their phytochemical constituents [29,30], which vary according to the agronomic conditions of growth and development of the crop, its geographical location, and the phenological stage and maturity of the plant, among others [31]. Until now, no publication has been found on the chemical composition and antioxidant activity of EOs and extracts of H. colombiana collected at its different phenological stages.…”

Natural Products Induce Different Anti-Tumor Immune Responses in Murine Models of 4T1 Mammary Carcinoma and B16-F10 Melanoma

“…It is widely distributed in nature, and its traditional use in the treatment of cancer occurs in various regions of the world, mainly on the American continent [1,2]. Anamu leaves have been extensively characterized phytochemically as well as for the diversity of additional uses of this plant [3][4][5]. Although dibenzyl trisulfide (DTS) has been considered the major active ingredient responsible for much of its antitumor activity, a drug containing this metabolite has not been developed due to its high toxicity and instability and its possible role as an inhibitor of intracellular cytochromes [6], suggesting that Anamu is the ideal candidate for the development of a polymolecular medicine.…”