Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Ghosh, Parthadeb; Das, Chandrima; Biswas, Soma; Nag, Sudip Kumar; Dutta, Alolika; Biswas, Manoj; Sil, Sayantan; Hazra, Labani; Ghosh, Chandreyi; Das, Shaktijit; Saha, Moumita; Mondal, Nasim; Mandal, Suprodip; Ghosh, Anirban; Karmakar, Srabani; Chatterjee, Sirshendu

doi:10.12688/f1000research.22966.1

Cited by 16 publications

(21 citation statements)

References 46 publications

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“…The plant may be used as nutritional purposes for its high availability and low cost. So, considering all the aspects it can be concluded that there is a huge scope for future study on Scoparia dulcis [1][2][3][46][47][48][49][50][51][52][53] .…”

Section: Discussionmentioning

confidence: 99%

Phytochemical, botanical and Ethnopharmacological study of Scoparia dulcis Linn. (Scrophulariaceae): A concise review

Sarkar¹,

Ghosh²,

Poddar³

et al. 2020

Pharma Innovation

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The ancient world had huge faith on the herbal medicines for curing various ailments. Scoparia dulcis Linn. is a medicinal herb of family Scrophulariaceae is considered to be one of such plants which have remarkable curative property. Scoparia dulcis is the commonly known as Sweet broom weed and is distributed throughout the tropical and subtropical region of the world. This herbaceous weed comprises of typical morphological characteristics which are of great use. Dried or fresh leaves, fruits and some other parts of plant serve the purpose of providing with essential components for the body metabolism. Some of the scientific studies demonstrated about the bioactive compounds present in the plant extract. These bioactive compounds were flavonoids, polyphenols, tannins and terpenoids. The fresh and the dried leaves of the herb showed its richness in ascorbic acid content. Scoparic acid, scopadulcic acid, scopadulciol, scopadulin and ammelinare is the major chemical components to which have contributed to the medicinal effect of the plant. Ethnomedicinal application of this herb revealed its uses for diabetes, jaundice, stomach problems, skin disease, fever, kidney stones, reproductory issues and piles. Evidences reported its flawless activity in anti-diabetic, anti-inflammatory, anti-ulcer, antioxidant, anti-microbial and analgesic illnesses. The current review is concerned with morphological, phytochemical, pharmacological, therapeutic and nutritional aspects of the plant Scoparia dulcis. This review may assist one to acquire encapsulated concept about the herb.

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Section: Discussionmentioning

confidence: 99%

Phytochemical, botanical and Ethnopharmacological study of Scoparia dulcis Linn. (Scrophulariaceae): A concise review

Sarkar¹,

Ghosh²,

Poddar³

et al. 2020

Pharma Innovation

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“…Now a day's plant protein is used as an alternative protein source for daily life.There is a large variation in the contribution made by plant proteins to the availability and intake of total dietary protein among populations both within the advanced regions of the world [16] . The primary protein which human beings get from leaves is necessary for vegetarians and poor people, because it is easily available and affordable or low cost with almost no side effects [17][18][19] . It can be stated that the combinations of plant proteins can provide a complete, necessary and balanced source of amino acids which effectively fulfills human physiological requirements [20][21][22] .…”

Section: Resultsmentioning

confidence: 99%

Quantification of total protein content from some traditionally used edible plant leaves: A comparative study

Sarkar¹,

Mondal²,

Ghosh³

et al. 2020

J. Med. Plants Stud.

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Protein is an important primary metabolite of our living systems. There are several studies based upon analysing the different, individual protein sources from various plant species are still on searching mode. On parallel with this context, traditionally edible plants has been proven to be an enriched source of protein based nutrition and largely utilized by most of the ethnic communities for their survival. The primary objective of the current research investigation is to estimate the total protein content from 33 traditionally used edible plant leaves categorized in trees, shrubs and herbs and to draw a comparative conclusion that which plant leaf contains better protein concentration. The protein content was estimated by UV-Vis spectrophotometric technique using the conventional Lowry's method. Bovine Serum Albumin (BSA) was used as standard reagent against which unknown protein concentration of plants had been estimated. All the proteins were extracted by using phosphate buffer (pH 7.4). From the results, it has been found that under tree category, Psidium guajava shows the highest (98.51 mg BSA Equivalent/ g of Fresh Weight) and Dillenia indica shows the lowest (13.73 mg BSAE/ g of FW) amount of protein content. In case of shrubs, Justicia adhatoda showed the maximum (86.37 mg BSAE/ g of FW) and Ocimum canum shows the minimum (10.59 mg BSAE/ g of FW) amount of protein content. Among the herbs, red Amaranthus viridis contains highest (97.43 BSAE/ g of FW) and Marsilea quadrifolia contains the lowest (15.04 mg BSAE/ g of FW) content of protein. The study results showed that Psidium guajava contains highest amount of protein among all the 33 plants. The study findings conclude that the protein content obtained from the leaves of different plant categories varies in their quantity and further supports the fact that plant leaves can be used as a potential source of nutrient consumption in near future.

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“…Compounds in nature reveal great diversity in terms of chemical structure and biological properties 45 . Studies carried out by Ghosh et al and Fitmawati et al concluded that medicinal plants are the best sources of phytochemicals and bioactive compounds that are useful for the development of drugs, antioxidants and those showing antidegenerative effects 46,47 . Several natural compounds exert their antioxidant effects through the activation of the key transcriptional regulation mechanism of Nrf2, allowing the coordinated expression of antioxidant enzymes such as NQO1 and HMOX-1.…”

Section: Discussionmentioning

confidence: 99%

Activation of the Keap1-Nrf2 pathway by specioside and the n-butanol extract from the inner bark of Tabebuia rosea (Bertol) DC

et al. 2020

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Background: A large number of chemical compounds exert their antioxidant effects by activation of key transcriptional regulatory mechanisms, such as the transcription factor Nrf2. The aim of this study was to evaluate the activation of the Keap1-Nrf2 pathway by both the n-butanol extract obtained from the inner bark of Tabebuia rosea (Bertol) DC and specioside isolated from this extract. Methods: The antioxidant activity of the extract and specioside isolated from the inner bark of T. rosea were evaluated using the oxygen radical absorbance capacity (ORAC) and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) techniques, whereas their effects on the viability of HepG2 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The effects of the compound and the extract on activating the Keap1-Nrf2 pathway were evaluated using a Nrf2 Transcription Factor Assay kit. Induction of the Nrf2-mediated antioxidant response genes HMOX-1 and NQO1 was evaluated by real-time PCR. The protective effects against H2O2-induced oxidative stress in HepG2 cells was determined as the percent protection using the MTT method. Results: Both the n-butanol extract and specioside exhibited activity at low concentrations without affecting cellular viability, since the cell viability was greater than 80% after 24 hours of exposure at each tested concentration. In addition, Nrf2 dissociated from Keap1 after treatment with the n-butanol extract at a concentration of 0.25 µg/mL after 4 hours of exposure. An increase in the Nrf2 level in the cytoplasm after 4 hours of exposure to 2 μM specioside was observed. Nrf2 levels stabilized in the nucleus 12 hours after stimulation with both specioside and the extract. After 6 hours of stimulation, both the extract and specioside induced the expression of HMOX-1 and NQO1. Conclusion: The n-butanol extract from the inner bark of T. rosea and specioside produced protective effects against H2O2-induced oxidative stress in HepG2 cells.

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Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Cited by 16 publications

References 46 publications

Phytochemical, botanical and Ethnopharmacological study of Scoparia dulcis Linn. (Scrophulariaceae): A concise review

Phytochemical, botanical and Ethnopharmacological study of Scoparia dulcis Linn. (Scrophulariaceae): A concise review

Quantification of total protein content from some traditionally used edible plant leaves: A comparative study

Activation of the Keap1-Nrf2 pathway by specioside and the n-butanol extract from the inner bark of Tabebuia rosea (Bertol) DC

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