Phytochemical Composition and Antimicrobial Activity of Daniella oliveri Extracts on Selected Clinical Microorganisms

Temitope, Osuntokun Oludare; Fasusi, Omolola H.; Ogunmodede, A; Thonda, A; Oladejo, Babayemi Olawale; Yusuf-Babatunde, A; Ige, Oladeji Oluremi

doi:10.9734/ijbcrr/2016/28764

Cited by 13 publications

(12 citation statements)

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“…Consumed either as a preventive measure or as a viable treatment solution, phytodrugs are not prone to certain side effects commonly associated with the use of synthetic drugs. The presence of bioactive compounds plants are being studied to help elucidate which of them individually or collectively are responsible for their antimicrobial activities thereby confirming the belief that local plants are the platform for traditional African medicine [36].…”

Section: Discussionmentioning

confidence: 93%

Aframomum melegueta [Roscoe] K. Schum) Ethanolic Seed Extract against Asymptomatic Bacteriuria Isolates from Pregnant Women Attending Antenatal Clinic in Ondo State, Nigeria

Osuntokun¹,

Balogun²,

Akele³

et al. 2021

IJPR

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The purpose of this research work is to compare the activity of medicinal plant(Aframomum melegueta) and conventional antibiotic against Asymptomatic Bacteriuria isolates from pregnant women attending ante-natal clinic in a major primary health center in Akoko, south ,Ondo state Nigeria. The target Asymptomatic Bacteriuria isolates which is inherent in pregnant women with no observable features. The rate of growth/ death of Asymptomatic Bacteriuria Isolates were x-rayed. Bacteria were isolated from Urine of pregnant women attending antenatal clinic in Public health care Akungba Akoko, Ondo state, Nigeria and were identified using conventional method. The antibiotic susceptibility test and antimicrobial screening of ethanol seed extract of Aframomum melegueta were determined using disc diffusion and agar well diffusion methods respectively. The eleven(11) bacteria identified were Klebsiella pneumoniae (three,3), Proteus mirabilis, Proteus vulgaris, Bacillus subtilis(Two,2),Pseudomonas aeruginosa, Enterobacter cloacae (Two,2), Escherichia coli (three,3), Bacillus cereus, Serratia mercesiens, Enterobacter aerogens. E. coli and klebsiella pneumoniae were the most common isolates. The second most common isotales was Enterobacter cloacae.. Klebsiella pneumonia and Proteus mirabilis were resistant to Ampicillin, Ceporex, Nalixadic acid and Septrin. E. coli, the most common isolate was sensitive to Gentamycin and most of the antibiotics used. The antimicrobial screening of ethanol seed extract of Aframomum melegueta shows zones of inhibition with diameter ranging from 1-25mm. Secondary metabolite screening indicates the presence of flavonoid, tannins, saponins, alkaloids, cardiac glycosides. Ultraviolet spectrophotometer was also used to determine the Growth dynamic /Death rate of the isolates, the addition of antibiotics to the organism at the 48th hour speed up the death rate of the isolates, the addition of ethanol seed extract at the 48th hour also speed up the death rate of the isolates from the urine samples. The results of this study validate the use of Aframomum melegueta seed in the traditional treatment of Asymptomatic bacteriuria in pregnant women.

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Section: Discussionmentioning

confidence: 93%

Aframomum melegueta [Roscoe] K. Schum) Ethanolic Seed Extract against Asymptomatic Bacteriuria Isolates from Pregnant Women Attending Antenatal Clinic in Ondo State, Nigeria

Osuntokun¹,

Balogun²,

Akele³

et al. 2021

IJPR

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“…29 Also, phytochemical screening of the plant revealed that it contains polyphenols, saponins, tannins, phytate, oxalate, saponin, steroid, alkaloids, and steroids, and it is used as an antidiabetic medicine. 26,27 This study suggested that phytochemicals soluble in methanol, exhibit some structural feature that resulted in binding and inhibition of PPL, and that phenolic compounds may be responsible for this inhibition The inhibitor possesses a non-competitive inhibitory effect on PPL, which indicated that active metabolites in P. guajavamay have reacted not only to free enzyme but also with the enzyme-substrate complex, and the binding constants are the same with a Ki value at all extract concentration at 2.644. This result indicated a kinetic basis for the finding that indicates that P. guajava leaves aqueous extracts to possess potential anti-obesity effects thus, can be used as an adjuvant in the treatment of obesity and other dyslipidemias.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Inhibitory Effect and Kinetic Studies of Selected Local Plant Extracts with Anti-Obesity Potentials on Pancreatic Lipase

2021

TJNPR

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One of the therapeutic strategies in managing obesity is the inhibition of pancreatic lipase, a key enzyme responsible for the catabolism of fats and triglycerides. In this study, the Anti-obesity efficacies of twenty-three local medicinal plant extracts (aqueous and methanol) were analyzed against porcine pancreatic lipase activity. Anti-lipase activity was determined by spectrophotometric method. The Methanol extract of Khayasenegalensis (Bark), Perseaamericana (root), Daniela oliveri (leaf), Psidiumguajava (leaf), showed more than 50 % inhibition on the enzyme activity. The aqueous extract of Khayasenegalensis (Bark) and Daniela oliveri (Bark) also showed more than 50 % inhibition on the enzyme activity. Kinetic Studies of PPL against the four effective methanol and aqueous extract showed that all the extracts exhibited a noncompetitive mode of inhibition by Michaelis-Menten non-linear regression and data by the double reciprocal plot. The Methanol extract of Khayasenegalensis (Bark), Perseaamericana (root), Daniela oliveri (leaf), Psidiumguajava (leaf), showed Km of 0.0182, 0.030, 0.0596, and 0.0398 mg/ml respectively. The aqueous extract of Khayasenegalensis (Bark) and Daniela oliveri (Bark) showed Km of 0.0204 and 0.0554 mg/ml. The result showed that Khayasenegalensis (Bark), Perseaamericana (root), Daniela oliveri (leaf), Psidiumguajava (leaf) can serve as a potential herbal alternative for the treatment of obesity. Further studies are required to elucidate the effectiveness of these active extracts and also an attempt should be explored to purify their active components to be used as safer and cheaper therapeutic agents in the future.

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“…The total colonies were counted using a colony counter. The count can also be done easily by dividing the plate into four quarters, the fi rst quarter counted fi rst and recorded then the second quarter till the fourth quarter which was noted and recorded (17).…”

Section: Total Bacteria Count Of Non-leguminous Endophytic Bacteria Isolates From Plant Samplementioning

confidence: 99%

Antimicrobial Spectrum, Growth/Killing Kinetics, Conventional/Molecular Assay of Characterizing Non-Leguminous Endophytic Bacteria and Fungi from Helianthus annuus, Carica papaya and Lycoperesicum solanum

Ot¹,

Vo²,

Bf³

et al. 2021

J Biomed Res Environ Sci

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The aim of this study is to comparative study between conventional and molecular assay of isolation, identification and characterization of non-leguminous endophytic bacteria and fungi in the leguminous root samples. The plant root samples, Helianthus annuus, Carica papaya and Lycoperesicum solanum (Sunflower root and stem, pawpaw root and stem, and tomato root and stem from Adekunle Ajasin University School farm, Akungba Akoko, Ondo state, Nigeria. The isolation of endophytic bacteria were performed using the conventional method of isolation (biochemical test) and characterization were done using both the conventional and molecular method of bacteria characterization. The antibiotic susceptibility test (Antibiogram) was observed using disc diffusion. The four bacteria identified were Bacillus cereus, Enterobacter sp. Actnomycoses sp. and Aeromonas sp. for conventional method and Fusarium solani, Fusarium vortecelium and Bacillus thuringiensis for molecular method as confirmatory point of view. In this study, all isolated organisms tends to be Gram positive using the gram staining technique. Antibiogram shows the zones of inhibition with diameter ranging from 0-20 mm, Enterobacter sp. were more sensitive to the various antibiotics used. Ultraviolet spectrophotometer was also used to determine the growth dynamic as well as the death rate of the isolates, the addition of antibiotics (ciprofloxacin) to the isolates at the 24th hour speed up the death rate of the isolates from non-leguminous endophytic bacteria. After the preliminary identification of the bacteria isolates and the confirmatory identification of both bacteria and fungi isolates of the non-leguminous endophytic microorganism, it was noted that the preliminary identification was only able to achieve the genus level of taxonomic characterization, While the molecular method confirm the molecular sub level identification of isolates depletes the absolute taxonomic identification and characterization to the sub-species level. The results of this study validates the use of molecular sequencing for the assay identification and characterization of non-leguminous endophytic bacteria and fungi as the easy and best mode of identification of both bacteria and fungi isolates as a veritable tools for research purposes.

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Phytochemical Composition and Antimicrobial Activity of Daniella oliveri Extracts on Selected Clinical Microorganisms

Cited by 13 publications

References 0 publications

Aframomum melegueta [Roscoe] K. Schum) Ethanolic Seed Extract against Asymptomatic Bacteriuria Isolates from Pregnant Women Attending Antenatal Clinic in Ondo State, Nigeria

Aframomum melegueta [Roscoe] K. Schum) Ethanolic Seed Extract against Asymptomatic Bacteriuria Isolates from Pregnant Women Attending Antenatal Clinic in Ondo State, Nigeria

In Vitro Inhibitory Effect and Kinetic Studies of Selected Local Plant Extracts with Anti-Obesity Potentials on Pancreatic Lipase

Antimicrobial Spectrum, Growth/Killing Kinetics, Conventional/Molecular Assay of Characterizing Non-Leguminous Endophytic Bacteria and Fungi from Helianthus annuus, Carica papaya and Lycoperesicum solanum

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