Phytochemical composition of Denhamia obscura (A. Rich.) Meisn. Ex Walp. root bark, seeds and leaves

Abstract

An investigation of the chemical composition of the root bark, seeds and leaves from the Australian plant, Denhamia obscura was carried out. Despite the traditional medicinal use of this plant by Indigenous communities in Australia and the comprehensive studies of other Denhamia species, the phytochemical profile of this plant has not been reported previously. Twelve known pentacyclic triterpenes, as well as seven abietane compounds were characterised. Two of the abietane compounds are new natural products, previously reported as synthetic compounds, obscurol and 13-methoxy-sempervir-6-ene. Friedelin was isolated from the root bark and leaves, with X-ray crystallography utilised to support its absolute configuration. The chemical compounds identified in this profile are consistent with other species now phylogenetically associated with the Denhamia genus.

Differentiating Dyes: A Spectroscopic Investigation into the Composition of Scarlet Bloodroot (Haemodorum coccineum R.Br.) Rhizome

“…Antimicrobial testing protocols for all microbes excluding H. influenzae have been previously reported [26] and were based on Clinical Laboratory Standards Institute (CLSI) for antimicrobial susceptibility testing [27,28]. In brief, the extract was tested against bacterial reference strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 25668), Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615) and yeast reference strain Candida albicans (ATCC 90029).…”