Skin aging occurs due to prolonged exposure to ultraviolet B (UVB) resulting in oxidative stress, which stimulates an increase in tyrosinase enzyme and melanin, causing hyperpigmentation. Chemical-based sunscreens often cause allergy and irritation, while hydroquinone (HQ) causes negative effects on the skin called ochronosis. Natural ingredients that are safe from side effects, such as strawberry (Fragaria ananassa), with active compounds of flavonoids, tannins and phenols with antioxidant and antiinflammation activity, can reduce melanogenesis. The aim of this study was to determine the effectiveness of 12,5% strawberry ethanol extract cream before and after UVB exposure in reducing levels of the tyrosinase enzyme and the amount of melanin in male guinea pigs. The research was a randomized post-test only control group design experimental study, using 30 male local strains guinea pigs, aged 3-4 months, weighing 300-350 grams, divided into three groups, consisted of 10 guinea pigs each. The control group (K0) was given UVB exposure and basic cream, treatment group 1 (K1) was given 12,5% strawberry extract cream before UVB exposure and treatment group 2 (K2) was given 12,5% strawberry extract cream after UVB exposure for 2 weeks. Skin samples were taken by biopsy and examined for tyrosinase enzyme levels using the ELISA method and melanin amount using the histopathological method of Masson-Fontana staining. The data was analyzed using One Way ANOVA and Post Hoc Tamhane. The mean levels of the tyrosinase enzyme in treatment group K1 and K2 (8,25 1,5 ng/ml and 17,47 3,09 ng/ml; p<0,05) decreased significantly compared to K0 (34,57 5,62 ng/ml). The mean amount of melanin in treatment group K1 and K2 (3,8 3,9%pixels and 5,13 1,53%pixels; p<0,05) decreased compared to K0 (21,16 10,21%pixels). There was significant difference in the decrease of tyrosinase enzyme in treatment group K1 and K2 (p<0,05), while no significant result was found in the decrease of melanin in treatment group K1 and K2 (p>0,05). In conclusion, 12.5% strawberry ethanol extract cream reduced tyrosinase enzyme level and melanin amount compared to control group applied with base cream, with better result when applied before UVB exposure (protection effect) than after UVB exposure (for treatment), with the same effectiveness in reducing the melanin amount but different effectiveness in reducing the tyrosinase enzyme.