Phytochemical constituents, antioxidant and antidiabetic activities of different extracts of the leaves, stem and root barks of Alstonia boonei: an in vitro and in silico study

Oyebode, Olajumoke A.; Erukainure, Ochuko L.; Ibeji, Collins U.; Koorbanally, Neil A.; Islam, Md. Shahidul

doi:10.1080/23818107.2019.1624980

Cited by 10 publications

(4 citation statements)

References 49 publications

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“…The increased hepatic amylase activity in inducing oxidative injury as depicted in Figure 6A further corroborates an elevated hepatic concentration of glucose in oxidative hepatotoxicity. Amylase catalyzes the disintegration of dietary carbohydrates to glucose and has been implicated in hyperglycemia (Oyebode et al, 2019;Sanni et al, 2019). Induction of oxidative injury also led to elevated activity of hepatic lipase (Figure 6B).…”

Section: Resultsmentioning

confidence: 99%

Cannabis sativa L. (var. indica) Exhibits Hepatoprotective Effects by Modulating Hepatic Lipid Profile and Mitigating Gluconeogenesis and Cholinergic Dysfunction in Oxidative Hepatic Injury

et al. 2021

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Cannabis sativa L. is a crop utilized globally for recreational, therapeutic, and religious purposes. Although considered as an illicit drug in most countries, C. sativa until recently started gaining attention for its medicinal application. This study sought to investigate the hepatoprotective effect of C. sativa on iron-mediated oxidative hepatic injury. Hepatic injury was induced ex vivo by incubating hepatic tissues with Fe2+, which led to depleted levels of reduced glutathione, superoxide dismutase, catalase and ENTPDase activities, triglyceride, and high-density lipoprotein–cholesterol (HDL-C). Induction of hepatic injury also caused significant elevation of malondialdehyde, nitric oxide, cholesterol, and low-density lipoprotein–cholesterol (LDL-C) levels while concomitantly elevating the activities of ATPase, glycogen phosphorylase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, amylase, and lipase. Treatment with the hexane, dichloromethane (DCM), and ethanol extracts of C. sativa leaves significantly (p < 0.05) reversed these levels and activities to almost near normal. However, there was no significant effect on the HDL-C level. The extracts also improved the utilization of glucose in Chang liver cells. High-performance liquid chromatography (HPLC) analysis showed the presence of phenolics in all extracts, with the ethanol extract having the highest constituents. Cannabidiol (CBD) was identified in all the extracts, while Δ-9-tetrahydrocannabinol (Δ-9-THC) was identified in the hexane and DCM extracts only. Molecular docking studies revealed strong interactions between CBD and Δ-9-THC with the β2 adrenergic receptor of the adrenergic system. The results demonstrate the potential of C. sativa to protect against oxidative-mediated hepatic injury by stalling oxidative stress, gluconeogenesis, and hepatic lipid accumulation while modulating cholinergic and purinergic activities. These activities may be associated with the synergistic effect of the compounds identified and possible interactions with the adrenergic system.

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Section: Resultsmentioning

confidence: 99%

Cannabis sativa L. (var. indica) Exhibits Hepatoprotective Effects by Modulating Hepatic Lipid Profile and Mitigating Gluconeogenesis and Cholinergic Dysfunction in Oxidative Hepatic Injury

et al. 2021

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“…Hypoxia is contemporary evidence for a stimulator of migration and invasion in a wide range of tumor cells in tumor microenvironments [ 10 , 11 ]. During carcinogenesis, low levels of oxygen may arise, boosting tumor cell proliferation, angiogenesis, and metastasis through a complex network involving HIF-1 and associated oxidative stress pathways [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, hypoxia is a state in which a tissue’s oxygenation is insufficient. When exposed to hypoxia, tissues/cells can be made to live by raising glycolysis rate [ 10 ]. However, hypoxic microenvironments are beneficial to cancer cells, tumor development, invasion, and angiogenesis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line

et al. 2022

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Background: This study comparatively assessed seven indigenous traditional tea plants on several attributes that included antioxidant, nutritional, caffeine contents, and cyclooxygenase activity. Methodology: Nutritional content of all tea plants were determined for energy, fat, carbohydrates, total sugars, dietary fiber and amino acids. Antioxidant potential and the antioxidant potentiating secondary metabolites were also measured and compared. Further, we investigated the tea plants for any role they would have on cyclooxygenase (COX) activity on cobalt chloride (CoCl2) induced human glioma cell lines (U87MG). Results: The tea plants were found non-cytotoxic at concentrations tested against the human Chang liver and HeK 293 kidney cells and were found to be naturally caffeine free. The lowest and highest extraction yield among the tea plants was 7.1% for B. saligna and 15.48% for L. scaberrimma respectively. On average, the flavonol content was 12 to 8 QE/g, ORAC 800 µmol TE/g, TEAC 150 µmol TE/g, FRAP 155 µmol AAE/g, polyphenols 40 mg GAE/g, flavanols 0.35 mg CE/g, flavonols 12 mg QE/g and total flavonoid content (TFC) 180 µg QE/mg. The COX activity has been found to be inhibited by a dose-dependent manner by L. scaberrimma, B. saligna and L. javanica. Conclusion: The results further support competitive value of tea plants and need for improved and further development.

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“…Increased α-amylase and lipase activities have been implicated as a major contributor to hyperglycemia as it breaks down dietary carbohydrate and lipid to glucose and free fatty acids (FFAs), respectively, which causes a rise in postprandial blood glucose and FFAs levels [47,48]. The increased activities in serum and pancreas of the untreated diabetic rats (Figure 4a,b) therefore indicate a postprandial rise in blood glucose and FFAs levels, thus contributing to hyperglycemia.…”

Section: Discussionmentioning

confidence: 99%

Ferulic acid improves glucose homeostasis by modulation of key diabetogenic activities and restoration of pancreatic architecture in diabetic rats

Salau

Erukainure

Olofinsan

et al. 2022

Fundamemntal Clinical Pharma

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There are increasing concerns on the rising cases of diabetes mellitus with type 2 diabetes (T2D) being of major interest as well as the cost of its treatment. Plant phenolic compounds are natural and potent antioxidants that have been widely reported for their antidiabetic activities properties, one of which is ferulic acid. The effect of ferulic acid (FA) on major diabetogenic activities and pancreatic architecture linked to T2D was investigated in T2D rats. T2D was induced in male Sprague–Dawley rats using the fructose‐streptozotocin model. Diabetic rats were treated with FA at 150 or 300 mg/kg bodyweight (bw). Normal control consisted of rats administered with food and water, while diabetic control consisted of untreated diabetic rats. Metformin was used as the standard drug. The rats were humanely sacrificed after 5 weeks of treatment. Their blood, liver, and pancreas were collected for analysis. Total glycogen content and carbohydrate metabolic enzymes activities were analyzed in the liver, while the pancreas and serum from blood were analyzed for oxidative stress biomarkers, purinergic and cholinergic enzyme activities, and amylase and lipase activities. The pancreatic tissue was further subjected to microscopic and histological examinations. FA caused a significant (p < 0.05) decrease in blood glucose level, with concomitant increase in serum insulin level. Treatment with FA also led to elevated levels of GSH, HDL‐c, SOD, and catalase activities, while concomitantly suppressing malondialdehyde, cholesterol, triglyceride, LDL‐c, NO, ALT, AST, creatinine, urea, and uric acid levels, acetylcholinesterase, ATPase, ENTPDase, 5′‐nucleotidase, lipase, glycogen phosphorylase, glucose‐6‐phosphatase, and fructose‐1,6‐biphosphatase activities. Histology analysis revealed an intact pancreatic morphology in FA‐treated diabetic rats. While transmission electron microscopy (TEM) analysis revealed an intact pancreatic ultrastructure and increased number of insulin granules in β‐cells. Taken together, these results portray that the antidiabetic potentials of ferulic acid involves modulation of major diabetogenic activities and maintenance of the pancreatic ultrastructure architecture.

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Phytochemical constituents, antioxidant and antidiabetic activities of different extracts of the leaves, stem and root barks of Alstonia boonei: an in vitro and in silico study

Cited by 10 publications

References 49 publications

Cannabis sativa L. (var. indica) Exhibits Hepatoprotective Effects by Modulating Hepatic Lipid Profile and Mitigating Gluconeogenesis and Cholinergic Dysfunction in Oxidative Hepatic Injury

Cannabis sativa L. (var. indica) Exhibits Hepatoprotective Effects by Modulating Hepatic Lipid Profile and Mitigating Gluconeogenesis and Cholinergic Dysfunction in Oxidative Hepatic Injury

Study on South African Indigenous Teas—Antioxidant Potential, Nutritional Content, and Hypoxia-Induced Cyclooxygenase Inhibition on U87 MG Cell Line

Ferulic acid improves glucose homeostasis by modulation of key diabetogenic activities and restoration of pancreatic architecture in diabetic rats

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