Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

Abstract

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

Phytopharmacological Assessment of Some Medicinal Plants of Thal Desert of Pakistan

“…Antioxidants, polyphenols, vitamins and rcarotenoids are the active and effective role in avoiding oxidative stress related diseases [29,30]. Similarly ,antioxidant activity of plant is determined by the concentration of pheolics present in plant [31][32][33]. Tocopherols and βcarotene are also the potent factor for antioidant activity potential [34].The variations in concentration of phytochemicals and nutrients is species specific [35].…”

The Phytochemical Screening and Antioxidant Potential of Cinnamomum Javanicum Blume Leaves From Central Kalimantan

“…Phytochemical screening of C. javanicum leaves by using the following standard methods [14][15][16][17] . The results of the phytochemical qualitative test of sintok lancang leaves showed the presence of alkaloids using Mayer's reagents 31 , flavonoids using the Shinoda test 32 , tannins and steroids (Table 1). 35&36 .…”

Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin

“…The preliminary phytochemical profiling of leaf powders of M. charantia and M. lucida to determine the major constituents (nitrogenous, polyphenolic, terpenic compound and glycosides) was done using a qualitative analysis based on coloring reactions and/or precipitation described by Senhaji et al (2020…”

“…An alternative is the use of medicinal plants. Many studies show that medicinal plants contain many biologically active secondary metabolites such as tannins (Chokki et al, 2020), terpenoid (Frezza et al, 2019), alkaloids (Vanderplanck and Glauser, 2018), glycosides (Pertuit et al, 2018), flavonoids, phenols (Frezza et al, 2019;Chokki et al, 2020) and other compounds which display various pharmacological activities: antioxidant, anti-inflammatory, anti-allergic, anti-cancer, analgesic, anti-diabetic, antibacterial, antifungal, antiviral activities (Ksouri et al, 2007;Forni et al, 2019;Senhaji et al, 2020). In the socio-economic and health context of developing countries, including Benin, the study of plants can lead to obtaining adequate and low-cost therapeutic responses, with proven scientific efficacy and optimal cultural acceptability.…”