Phytochemical evaluation of Ocimum basilicum (Sweet basil) leaves collected from Abakaliki-Nigeria, using Gas chromatography-Mass Spectrometry.

AJ, Uraku

doi:10.19046/abp.v03i01.07

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Earlier reports of free radical scavenging properties of P. emblica, O. basilicum, B. monnieri, T. sinensis, A. racemosus, and W. somnifera divulges as primary antioxidants to protect cells from oxidative stress [ 16 , 29 , [57] , [58] , [59] , [60] , [61] ]. The stable free radical DPPH measures the chemical ability of the combinatorial extracts to get reduced by donating hydrogen to the radical [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Dual herbal combination of Withania somnifera and five Rasayana herbs: A phytochemical, antioxidant, and chemometric profiling

Bhargavi¹,

Shankar²

2021

Journal of Ayurveda and Integrative Medicine

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Background Traditional medicine adequately emphasis plant resources for addressing a wide variety of human ailments by utilizing the naturally occurring phytoconstituents; in particular medicinal plants or parts of plants in combination have prodigious antioxidant potentials. Objective The present study aims to analyze methanolic extract of W. somnifera (W) individually, and in dual combination with five Rasayana herbs P. emblica - (W:P), B. monnieri - (W:B), T. sinensis - (W:T), O. basilicum - (W:O), A. racemosus - (W:A) in three dual ratios [4:1, 1:1, and 1:4]. The efficacy of the combinations is assessed based on their chemometric profiling. Material and methods A total of 15 dual combinatorial methanolic extracts together with W. somnifera were evaluated for their preliminary phytochemical profiles, antioxidant potentials using DPPH and FRAP assays. Five dual samples were selected and analyzed for High-Performance Thin-Layer Chromatography (HPTLC) image-based chemometric profiling followed by Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA)-Heatmaps. Results Qualitative phytochemical analysis of combinatorial extracts exhibited a richness for a variety of phytoconstituents. The antioxidant activity was significantly higher for DPPH IC 50 (μg/mL): W = 11.56 ± 3.69; W:P/1:4 = 7.89 ± 1.52; W:O/1:4 = 8.995 ± 2.64 and FRAP (μM FeE /g ): W = 4.56 ± 0.54; W:P/1:4 = 138.34 ± 9.25; W:O/1:4 = 15.32 ± 1.64. Chemometric data acquisition displayed improved secondary metabolite close cluster combination with W:O/1:4 and W:P/1:4 than W. somnifera (W) alone. Conclusion The dual herbal combinatorial study revealed that the methanolic combinatorial extracts phytoconstituents correlated with an increase in the antioxidant potential and would serve as a promising source for phytomedicine.

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Section: Discussionmentioning

confidence: 99%

Dual herbal combination of Withania somnifera and five Rasayana herbs: A phytochemical, antioxidant, and chemometric profiling

Bhargavi¹,

Shankar²

2021

Journal of Ayurveda and Integrative Medicine

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The Comparison of Gc-MS Data of Leaves of Aloe Vera (A. Barbadensis Mill) Plant Grown in Different Soil Combinations With Chemical Lab Wastes

Rai

Chatrath

2021

gctl

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Purpose of the study: The main purpose of this study is to do the comparison of the chemical constituents present in the leaves of Aloe Vera (A. barbadensis Mill) plant grown in different soil combinations using Gas Chromatography- Mass Spectrometry (GC-MS) data analysis. Plants were grown in two different combinations of soil, one in normal soil and the other in the combination of normal soil and solid chemical waste obtained from the chemistry laboratory of an educational institute. Methodology: One Aloe Vera (A. barbadensis Mill) plant was grown in normal soil without adding any compost or any fertilizer to the soil. The other plant was grown in soil, which was combined with the solid chemical waste collected from the chemistry laboratory. After observing the morphological characters of the plants, plants were studied for the chemical constituents present in them by using GC-MS data analysis technique. Main Findings: Growth of plants depends on the soil composition, physical characters and the surrounding environment. How the variation in chemical composition of soil affects the chemical constituents of plant leaves, has been discussed in this paper. According to morphological characters the Aloe Vera (A. barbadensis Mill) plant grown with soil combined with solid chemical waste has shown better results compared with the one grown in normal soil. GC-MS results also indicated variation in the chemical constitution of plant leaves taken for the research experiment. Applications of this study: This study has helped to understand that the soil environment and soil nutrients are largely responsible for the changes in chemical constituents of plants. This study can be applied to the other plants as well. Novelty/Originality of this study: In place of fertilizers, solid chemical waste from the laboratory was used for the research purpose. The method is useful and if implemented on a large scale, will help to curb pollution caused by educational institutes to some extent. This kind of research is not done previously by any other researcher.

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Phytochemistry and GC-MS screening and biocidal potentiality of clove (Syzygium aromaticum L.) pods against mosquitos’ larvae

Elbashir¹,

Kehail²,

Abdelrahim³

et al. 2021

Int. J. Sci. Res. Arch.

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Many measures have been used for mosquito control, including the elimination of breeding places, exclusion via window screens and mosquito nets in addition to natural products including clove (Syzygium aromaticum). This study was run at University of Gezira, Sudan, to run phytochemical and GC-MS screening for clove pods before used it as mosquito control agent. The standard methods, materials and devices were used to screen the phytochemical components and the chemical constituents (GC-MS). The WHO protocol for testing the susceptibility of mosquito’s larvae to insecticides was followed in bioassay. The aqueous and the ethanol extracts from clove pods were prepared and used against Anopheles, Culex and Aedes larvae. The results showed that, Aedes mosquito was relatively more susceptible (LC50= 498 mg/L) to clove aqueous extract than Anopheles (LC50= 561 mg/L) and Culex (LC50= 615 mg/L), and similar findings were observed for clove pods ethanol extract, which is relatively more potent than the aqueous extract. The biocidal activity can be attributed to the presence of the detected saponins, flavonoids, tannins and alkaloids. The GC-MS for the ethanol extract showed that, the principal compounds were Eugenol (81%) and caryophyllene (4.65%). Further studies should be run to improve knowledge about how to use this natural product in more economic trends.

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Phytochemical evaluation of Ocimum basilicum (Sweet basil) leaves collected from Abakaliki-Nigeria, using Gas chromatography-Mass Spectrometry.

Cited by 3 publications

References 6 publications

Dual herbal combination of Withania somnifera and five Rasayana herbs: A phytochemical, antioxidant, and chemometric profiling

Dual herbal combination of Withania somnifera and five Rasayana herbs: A phytochemical, antioxidant, and chemometric profiling

The Comparison of Gc-MS Data of Leaves of Aloe Vera (A. Barbadensis Mill) Plant Grown in Different Soil Combinations With Chemical Lab Wastes

Phytochemistry and GC-MS screening and biocidal potentiality of clove (Syzygium aromaticum L.) pods against mosquitos’ larvae

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