Phytochemical Investigation, Gc-MS Profile and Antimicrobial Activity of a Medicinal Plant Ruta Graveolens L. From Ethiopia

Abstract

Objective: This study was designed to screen the phytochemicals present in various solvents extracts of Ruta graveolens (Rue) and furthermore to investigate their antimicrobial activity. Methods:The leaves, stems and seeds of Rue were extracted using four different solvents viz. ethanolic, methanolic, chloroform, and aqueous of varying polarity. The phytochemical screening was carried out qualitatively and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was performed to identify major phytoconstituents present in the methanolic leaf extract. The antimicrobial effect of extracts was evaluated against six microbial strains namely Bacillus subtillis, Escherichia coli, Proteus vulgaris, Candida albicans, Candida tropicalis and Micrococcus luteus with disc diffusion method.Results: Phytochemical analysis revealed the presence of various secondary metabolites such as flavonoids, alkaloids, terpenoids, saponins and carotenoid. The methanolic leaf extract showed the presence of both tannin and phenolic contents in the higher amount, whereas aqueous extract displayed in the least amount. GC-MS analysis of methanolic leaf extract revealed the presence of approximately 26 phytochemical constituents. The antimicrobial assay revealed that B. subtilis showed a high zone of inhibition (20 mm) at 200 mg/ml of methanolic extract. However, E. coli and C. tropicalis did not show any zone of inhibition against each solvent extract. Conclusion:In conclusion, secondary metabolites present in the extracts have biological activities which warrant further to evaluate in vivo pharmacological studies.

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

“…The GC-MS analyses the eluted compound at different times to identify phytochemical compounds. Large compounds changing into small compounds give rise to visual peaks at different ratios, and the fingerprints of the detected compounds can be identified from the data library [40,41]. TLC was carried out using different types of solvent systems with varying polarity.…”

Phytochemical Evaluation of Tiliacora Racemosa Colebr. Using Gas Chromatography - Mass Spectrometry (Gc-Ms)

“…Alkaloids possess antibacterial and antidiabetic properties [16,17]. Flavonoids are known to possess anticancer, antiviral, antiinflammatory and antioxidant properties [18,19]. Phenolic compounds are well known to possess biological activities such as antioxidant, antidiabetic, hepatoprotective, anti-inflammatory, antimicrobial and anticancer [20,21].…”

Phytochemical, Gas Chromatography With Mass Spectrometry Analysis of Andrographis Serpyllifolia Methanol Leaf Extract and Its Antioxidant and Antibacterial Activities

“…The stimulation of SOD activity along with CAT seemed to play a protective role against membrane damage as Cu is particularly toxic to membranes [28]. SOD and CAT in Tylophora pauciflora were found to be 29.78±0.57 units/mg protein and 39.87±0.51 µmole of H 2 O 2 consumed/min/mg proteins, respectively [29]. GPx and GST levels in methanolic leaf extracts shows the high level of GPx (231.4 ± 1.46 µg of glutathione oxidized/min/mg protein) and 153.1 ± 1.43 µmoles of CDNB -GSH conjugate formed/min/mg protein.…”