Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece

Barda, Christina; Ćirić, Ana; Sokóvić, Marina; Tsoukalas, Michail; Skaltsa, Helen

doi:10.1016/j.bse.2018.06.009

Cited by 7 publications

(9 citation statements)

References 26 publications

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“…& SM. (Asteraceae) flowering aerial parts, expressed the best activity against Gram‐negative bacteria Enterococcus faecalis ( MIC =0.02 mg/mL; MBC =0.03 mg/mL) and P. aeruginosa ( MIC =0.03 mg/mL; MBC =0.06 mg/mL), and molds A. versicolor and T. viride ( MIC =0.045 mg/mL; MFC =0.3 mg/mL) [32] . The α ‐methylene‐ γ ‐lactone moiety is of particular importance for the manifestation of the crepiside E antimicrobial activity, i. e ., free exocyclic methylene group from this moiety causes the alkylation of cell membrane components and enzymes of microorganisms [33,34] .…”

Section: Resultsmentioning

confidence: 99%

“…(Asteraceae) flowering aerial parts, expressed the best activity against Gram-negative bacteria Enterococcus faecalis (MIC = 0.02 mg/mL; MBC = 0.03 mg/mL) and P. aeruginosa (MIC = 0.03 mg/mL; MBC = 0.06 mg/mL), and molds A. versicolor and T. viride (MIC = 0.045 mg/mL; MFC = 0.3 mg/mL). [32] The α-methylene-γ-lactone moiety is of particular importance for the manifestation of the crepiside E antimicrobial activity, i. e., free exocyclic methylene group from this moiety causes the alkylation of cell membrane components and enzymes of microorganisms. [33,34] However, similar antimicrobial activity of 8-epiixerisamine A (which does not contain free α-methylene group, but Nmethyl-proline instead) represents interesting finding that demands further studies of antimicrobial mode of action of this SLs.…”

Section: [C]mentioning

confidence: 99%

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Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones

Milutinović

Matić

Stanojković

et al. 2022

Chemistry & Biodiversity

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Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8‐epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8‐epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC‐5 cells.

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Section: Resultsmentioning

confidence: 99%

Section: [C]mentioning

confidence: 99%

Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones

Milutinović

Matić

Stanojković

et al. 2022

Chemistry & Biodiversity

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“…In contrast to the large diversity of sesquiterpenes reported, only a limited number of flavonoids have been observed in the genus Crepis. These are mainly luteolin derivatives, with luteolin itself (76) being the most common one, present in twenty-one species. Additionally, luteolin-7-O-glucoside (80) and luteolin 7-O-glucuronide (81) are present in several species, eighteen and thirteen, respectively, whereas luteolin 7-O-gentiobioside (82) and luteolin 4 -O-glucoside (83) occur in few cases.…”

Section: Flavonoidsmentioning

confidence: 99%

“…The HPLC-MS analysis of the aerial parts and roots of C. foetida L. subsp. rhoeadifolia showed the presence of chlorogenic acid (104) and luteonin (76). The tested extracts showed antioxidant activity in assays of DPPH (IC 50 = 0.26-1.54 mg/mL) and of the reactive substances of thiobarbituric acid (TBARS) (MDA level = 4.54-19.15 nmol/mL), thus suggesting that the activity shown by the extracts of this plant is linked to its polyphenolic content [46].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Ethnobotany, Phytochemistry, Biological, and Nutritional Properties of Genus Crepis—A Review

Badalamenti

Sottile

Bruno

2022

Plants

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The genus Crepis L., included within the Asteraceae family, has a very wide distribution, expanding throughout the northern hemisphere, including Europe, northern Africa, and temperate Asia. This genus has a fundamental value from biodynamic and ecological perspectives, with the different species often being chosen for soil conservation, for environmental sustainability, and for their attraction towards pollinating species. Furthermore, various species of Crepis have been used in the popular medicine of several countries as medicinal herbs and food since ancient times. In most cases, the species is consumed either in the form of a decoction, or as a salad, and is used for its cardiovascular properties, as a digestive, for problems related to sight, for the treatment of diabetes, and for joint diseases. This literature review, the first one of the Crepis genus, includes publications with the word ‘Crepis’, and considers the single metabolites identified, characterised, and tested to evaluate their biological potential. The various isolated compounds, including in most cases sesquiterpenes and flavonoids, were obtained by extracting the roots and aerial parts of the different species. The secondary metabolites, extracted using traditional (solvent extraction, column chromatography, preparative thin layer chromatography, preparative HPLC, vacuum liquid chromatography), and modern systems such as ultrasounds, microwaves, etc., and characterised by mono- and bi- dimensional NMR experiments and by HPLC-MS, have a varied application spectrum at a biological level, with antimicrobial, antioxidant, antidiabetic, antitumor, antiviral, antiulcer, phytotoxic, and nutritional properties having been reported. Unfortunately, in vitro tests have not always been accompanied by in vivo tests, and this is the major critical aspect that emerges from the study of the scientific aspects related to this genus. Therefore, extensive investigations are necessary to evaluate the real capacity of the different species used in food, and above all to discover what the different plants that have never been analysed could offer at a scientific level.

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“…Extracts obtained from the different parts of the Crepis spp. and their isolated constituents have undergone only limited evaluation as antitumor, anti-inflammatory, antiviral, antimicrobial, antiulcer, antioxidant and nutritional agents [ 5 , 6 ]. Moreover, within the Crepis genus, few edible representatives appear, such as C. bulbosa , C. capillaris , C. commutata , C. foetida , C. setosa and C. vesicaria [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

A Bio-Guided Screening for Antioxidant, Anti-Inflammatory and Hypolipidemic Potential Supported by Non-Targeted Metabolomic Analysis of Crepis spp.

et al. 2022

Self Cite

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This study was designed to evaluate the chemical fingerprints and the antioxidant, anti-inflammatory and hypolipidemic activity of selected Crepis species collected in Greece, namely, C. commutata, C. dioscoridis, C. foetida, C. heldreichiana, C. incana, C. rubra, and Phitosia crocifolia (formerly known as Crepis crocifolia). For the phytochemical analyses, sample measurements were carried out by using nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography coupled with mass spectrometry (LC-MS). Τhe extracts were evaluated both in vitro (radical scavenging activity: DPPH assay and total phenolic content: Folin–Ciocalteu) and in vivo (paw edema reduction and hypolipidemic activity: experimental mouse protocols). Among the tested extracts, C. incana presented the highest gallic acid equivalents (GAE) (0.0834mg/mL) and the highest antioxidant activity (IC50 = 0.07 mg/mL) in vitro, as well as the highest anti-inflammatory activity with 32% edema reduction in vivo. Moreover, in the hypolipidemic protocol, the same extract increased plasma total antioxidant capacity (TAC) by 48.7%, and decreased cholesterol (41.3%) as well as triglycerides (37.2%). According to fractionation of the extract and the phytochemical results, this biological effect may be associated with the rich phenolic composition; caffeoyl tartaric acid derivatives (cichoric and caftaric acid) are regarded as the most prominent bioactive specialized metabolites. The present study contributes to the knowledge regarding the phytochemical and pharmacological profile of Crepis spp.

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Phytochemical investigation of Crepis incana Sm. (Asteraceae) endemic to southern Greece

Cited by 7 publications

References 26 publications

Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones

Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones

Ethnobotany, Phytochemistry, Biological, and Nutritional Properties of Genus Crepis—A Review

A Bio-Guided Screening for Antioxidant, Anti-Inflammatory and Hypolipidemic Potential Supported by Non-Targeted Metabolomic Analysis of Crepis spp.

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